Skip to main content

Advertisement

SpringerLink
Log in

Biosynthesized silver nanoparticles using floral extract of Chrysanthemum indicum L.—potential for malaria vector control

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

Mosquitoes transmit serious human diseases, causing millions of deaths every year. The use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides synthesized of natural products for vector control have been a priority in this area. In the present study, silver nanoparticles (Ag NPs) were green-synthesized using a floral extract of Chrysanthemum indicum screened for larvicidal and pupicidal activity against the first to fourth instar larvae and pupae of the malaria vector Anopheles stephensi mosquitoes. The synthesized Ag NPs were characterized by using UV–vis absorption, X-ray diffraction, transmission electron microscopy, and energy-dispersive X-ray spectroscopy techniques. The textures of the yielded Ag NPs were found to be spherical and polydispersed with a mean size in the range of 25–59 nm. Larvae and pupae were exposed to various concentrations of aqueous extract of C. indicum and synthesized Ag NPs for 24 h, and the maximum mortality was observed from the synthesized Ag NPs against the vector A. stephensi (LC50 = 5.07, 10.35, 14.19, 22.81, and 35.05 ppm; LC90 = 29.18, 47.15, 65.53, 87.96, and 115.05 ppm). These results suggest that the synthesized Ag NPs have the potential to be used as an ideal eco-friendly approach for the control of A. stephensi. Additionally, this study provides the larvicidal and pupicidal properties of green-synthesized Ag NPs with the floral extract of C. indicum against vector mosquito species from the geographical location of India.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    Article  CAS  Google Scholar 

  • Alder HL, Rossler EB (1977) Introduction to probability and statistics. Freeman, San Francisco, 246p

    Google Scholar 

  • Awwad AM, Salem NM, Abdeen AO (2013) Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. Int J Ind Chem 4:29

    Article  Google Scholar 

  • Burda C, Chen X, Narayanan R, El-Sayed MA (2005) The chemistry and properties of nanocrystals of different shapes. Chem Rev 105:1025–1102

    Article  CAS  Google Scholar 

  • Chapagain BP, Saharan V, Wiesman Z (2008) Larvicidal activity of saponins from Balanites aegyptiaca callus against Aedes aegypti mosquito. Bioresour Technol 99:1165–1168

    Article  CAS  Google Scholar 

  • Debnath T, Jin HL, Hasnat MA, Kim Y, Samad NB, Park PJ, Lim BO (2013) Antioxidant potential and oxidative DNA damage preventive activity of Chrysanthemum indicum. J Food Biochem 37:440--448

  • Edeoga HO, Okwu DE, Mbaebie BO (2005) Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 4:685–688

    Article  CAS  Google Scholar 

  • Finney DJ (1971) Probit analysis. Cambridge University Press, London

    Google Scholar 

  • Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang Y, Shao W, He N, Hong J, Chen C (2007) Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 18:105104

  • Jang YS, Kim MK, Ahn YJ, Lee HS (2002) Larvicidal activity of Brazilian plants against Aedes aegypti and Culex pipienspallens (Diptera: Culicidae). Agric Chem Biotechnol 45:131–134

    Google Scholar 

  • Jha AK, Prasad K, Kulkarni AR (2009) Plant system: nature’s nanofactory. Colloids Surf B: Biointerfaces 73:219–223

    Article  CAS  Google Scholar 

  • Jung EK (2009) Chemical composition and antimicrobial activity of the essential oil of Chrysanthemum indicum against oral bacteria. J Bacteriol Virol 19:61–69

    Article  Google Scholar 

  • Kesharwani J, Yoon KY, Hwang J, Rai M (2009) Phytofabrication of silver nanoparticles by leaf extract of Datura metel: hypothetical mechanism involved in synthesis. J Bionanoscience 3:39–44

    Article  CAS  Google Scholar 

  • Kovendan K, Murugan K, Vincent S (2012) Evaluation of larvicidal activity of Acalypha alnifolia Klein ex Willd. (Euphorbiaceae) leaf extract against the malarial vector, Anopheles stephensi, dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 110:571–581

    Article  Google Scholar 

  • Kumar A, Singh SP, Bhakuni RS (2005) Secondary metabolites of Chrysanthemum genus and their biological activities. Curr Sci 89:1489–1501

    CAS  Google Scholar 

  • Li S, Shen Y, Xie A, Yu X, Qui L, Zhang L, Zhang Q (2007) Green synthesis of silver nanoparticles using Capsicum annum L. extract. Green Chem 9:852–858

    Article  CAS  Google Scholar 

  • Mathew N, Anitha MG, Bala TS, Sivakumar SM, Narmadha R, Kalyanasundaram M (2009) Larvicidal activity of Saraca indica, Nyctanthes arbor-tristis, and Clitoria ternatea extracts against three mosquito vector species. Parasitol Res 104:1017–1025

    Article  Google Scholar 

  • Mohanpuria P, Rana NK, Yadav SK (2008) Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 10:507–517

    Article  CAS  Google Scholar 

  • Mulla MS, Thavara U, Tawatsin A, Chompoosri J, Zaim M, Su T (2003) Laboratory and field evaluation of novaluron, a new acylurea insect growth regulator, against Aedes aegypti (Diptera: Culicidae). J Vector Ecol 28:241–254

    Google Scholar 

  • Nivsarkar M, Cherian B, Padh H (2001) Alpha-terthienyl: a plant-derived new generation insecticide. Curr Sci 81:667–672

    CAS  Google Scholar 

  • Patil SV, Salunke BK, Patil CD, Salunkhe RB, Gavit P, Maheshwari VL (2010) Potential of extracts of the tropical plant Balanites aegyptiaca (L) del. (Balanitaceae) to control the mealy bug, Maconellicoccus hirsutus (Homoptera: Pseudococcidae). Crop Prot 29:1293–1296

    Article  Google Scholar 

  • Prakash P, Gnanaprakasam P, Emmanuel R, Arokiyaraj S, Saravanan M (2013) Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates. Colloids Surf B: Biointerfaces 108:255

    Article  CAS  Google Scholar 

  • Priyadarshini K, Murugan K, Panneerselvam C, Ponarulselvam S, Hwang JS, Nicoletti M (2012) Biolarvicidal and pupicidal potential of silver nanoparticles synthesized using Euphorbia hirta against Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 111:997–1006

    Article  Google Scholar 

  • Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83

    Article  CAS  Google Scholar 

  • Sathyavathi R, Balamurali Krishna M, Venugopal Rao, Saritha R, Narayana Rao D (2010) Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3:1

    Article  Google Scholar 

  • Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag and bimetallic Au core-Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 275:496–502

    Article  CAS  Google Scholar 

  • Sharma P, Mohan L, Srivastava CN (2009) Amaranthus oleracea and Euphorbia hirta: natural potential larvicidal agents against the urban Indian malaria vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 106:171–176

    Article  Google Scholar 

  • Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI (2005) The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature 434:214–217

    Article  CAS  Google Scholar 

  • SPSS Inc (2007) SPSS 16.0 for Windows, Rel. 16.0.1.1

  • Suganya A, Murugan K, Kovendan K, Mahesh Kumar P, Hwang JS (2013) Green synthesis of silver nanoparticles using Murraya koenigii leaf extract against Anopheles stephensi and Aedes aegypti. Parasitol Res 112:1385–1397

    Article  Google Scholar 

  • Suman TY, Elumalai D, Kaleena PK, Radhika Rajasree SR (2013) GC–MS analysis of bioactive components and synthesis of silver nanoparticle using Ammannia baccifera aerial extract and its larvicidal activity against malaria and filariasis vectors. Ind Crops Prod 47:239–245

    Article  CAS  Google Scholar 

  • Wiseman Z, Chapagain BP (2006) Larvicidal activity of saponin containing extracts and fractions of fruit mesocarp of Balanites aegyptiaca. Fitoterapia 77:420–424

    Article  Google Scholar 

  • World Health Organisation (1981) Instruction for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO- VBC 81:807–962

    Google Scholar 

Download references

Acknowledgments

The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group project NO (RG-1435-071).

Author information

Author notes

    Authors and Affiliations

    1. Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea

      Selvaraj Arokiyaraj

    2. P.G. Research and Department of Advanced Zoology and Biotechnology, Centre for Environmental Research and Development, Loyola College, Nungambakkam, Chennai, 600 034, Tamil Nadu, India

      Vannam Dinesh Kumar & Savariar Vincent

    3. Department of Biotechnology, Vel Tech High Tech Dr. RR Dr. SR Engineering College, Avadi, Chennai, 600 062, Tamil Nadu, India

      Selvaraj Arokiyaraj & Vijay Elakya

    4. Deparment of Biotechnology, Rajalakshmi Engineering College, Chennai, 602 105, Tamil Nadu, India

      Tamilselvan Kamala

    5. Department of Animal Nutrition and Physiology, National Institute of Animal Science, RDA, Suwon, 441-706, Republic of Korea

      Sung Kwon Park

    6. Department of Physics, Fatima College, Madurai, 625 018, Tamil Nadu, India

      Muthiah Ragam

    7. Department of Medical Microbiology and Immunology, Institute of Biomedical Sciences, College of Health Science, Mekelle University, PO Box 231, Mekelle, Ethiopia

      Muthupandian Saravanan

    8. Nanotechnology Centre, University of Bahrain, PO Box 32038, Sakhir, Kingdom of Bahrain

      Mohomad Bououdina

    9. Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

      Mariadhas Valan Arasu

    10. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

      Kalimuthu Kovendan

    Authors
    1. Selvaraj Arokiyaraj
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Vannam Dinesh Kumar
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Vijay Elakya
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Tamilselvan Kamala
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Sung Kwon Park
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Muthiah Ragam
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Muthupandian Saravanan
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Mohomad Bououdina
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Mariadhas Valan Arasu
      View author publications

      You can also search for this author in PubMed Google Scholar

    10. Kalimuthu Kovendan
      View author publications

      You can also search for this author in PubMed Google Scholar

    11. Savariar Vincent
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Kalimuthu Kovendan.

    Additional information

    Responsible editor: Philippe Garrigues

    V. Dineshkumar contributed equally to this work.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Arokiyaraj, S., Dinesh Kumar, V., Elakya, V. et al. Biosynthesized silver nanoparticles using floral extract of Chrysanthemum indicum L.—potential for malaria vector control. Environ Sci Pollut Res 22, 9759–9765 (2015). https://doi.org/10.1007/s11356-015-4148-9

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s11356-015-4148-9

    Keywords

    Search

    Navigation