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EPR and LC-MS studies on the mechanism of industrial dye decolorization by versatile peroxidase from Bjerkandera adusta

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Abstract

The mechanisms of industrial dye transformation by versatile peroxidase were elucidated. Purified versatile peroxidase from Bjerkandera adusta was able to decolorize different classes of dyes including azo and phthalocyanines, but unable to transform any of the anthraquinones tested. Kinetic constants for selected dyes were determined and the transformation products were analyzed by EPR spectroscopy and mass spectrometry. The EPR and MS analyses of the enzymatic decolorization products showed the cleavage of the azo bond in azo dyes and the total disruption of the phthalocyaninic ring in phthalocyanine dyes. The EPR analysis on two copper-containing dyes, reactive violet 5 (azo) and reactive blue 72 (phthalocyanine), showed that the transformation can or not break the metal-ion coordination bond according the dye nature. The role of the catalytic Trp172 in the dye transformation by a long-range electron transfer pathway was confirmed and the oxidation mechanisms are proposed and discussed.

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Acknowledgments

We thank Rosa Roman for her technical assistance. This project was funded by the National Council of Science and Technology of Mexico (CONACYT) and the Italian MIUR PRIN 2009 STNWX3 and the Italian CSGI Consortium.

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Authors and Affiliations

  1. Department of Biotechnology, Chemistry, and Pharmacy, University of Siena, Siena, Italy

    Maria Camilla Baratto, Rebecca Pogni & Riccardo Basosi

  2. Center for Nanosciences and Nanotechnology-UNAM, Km 107 Carretera Tijuana-Ensenada, Ensenada, Baja California, 22860, Mexico

    Karla Juarez-Moreno & Rafael Vazquez-Duhalt

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  1. Maria Camilla Baratto
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  2. Karla Juarez-Moreno
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  3. Rebecca Pogni
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  4. Riccardo Basosi
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  5. Rafael Vazquez-Duhalt
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Correspondence to Rafael Vazquez-Duhalt.

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Baratto, M.C., Juarez-Moreno, K., Pogni, R. et al. EPR and LC-MS studies on the mechanism of industrial dye decolorization by versatile peroxidase from Bjerkandera adusta . Environ Sci Pollut Res 22, 8683–8692 (2015). https://doi.org/10.1007/s11356-014-4051-9

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