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Soil genotoxicity assessment—results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization

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Abstract

The Vicia micronucleus assay was standardized in an international protocol, ISO 29200, “Assessment of genotoxic effects on higher plants—Vicia faba micronucleus test,” for soil or soil materials (e.g., compost, sludge, sediment, waste, and fertilizing materials). The aim of this interlaboratory study on the Vicia micronucleus assay was to investigate the robustness of this in vivo assay in terms of its applicability in different countries where each participant were asked to use their own seeds and reference soil, in agreement with the ISO 29200 standard. The ISO 29200 standard protocol was adopted for this study, and seven laboratories from three countries (France, Italy, and Brazil) participated in the study. Negative and positive controls were correctly evaluated by 100 % of the participants. In the solid-phase test, the micronucleus frequency (number of micronuclei/1,000 cells) varied from 0.0 to 1.8 for the negative control (i.e., Hoagland’s solution) and from 5.8 to 85.7 for the positive control (i.e., maleic hydrazide), while these values varied from 0.0 to 1.7 for the negative control and from 14.3 to 97.7 for the positive control in the case of liquid-phase test. The variability in the data obtained does not adversely affect the robustness of the protocol assessed, on the condition that the methodology described in the standard ISO 29200 is strictly respected. Thus, the Vicia micronucleus test (ISO 29200) is appropriate for complementing prokaryotic or in vitro tests cited in legislation related to risk assessment of genotoxicity potential.

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Acknowledgments

This work was supported by grants from the “Agence de l'Environnement et de la Maîtrise de l'Energie” (ADEME) and the “Conseil Régional de Lorraine”. Brazilian seeds were supplied by the state agronomy company Empresa de Pesquisa Agropecuária e Difusão de Tecnologia de Santa Catarina, Santa Catarina State, Brazil (EPAGRI).

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Authors and Affiliations

  1. Laboratoire Interdisciplinaire des Environnements Continentaux, Université de Lorraine (LIEC), CNRS UMR 7360, 8, Rue du General Delestraint, Metz, 57070, France

    Sylvie Cotelle, Adrien Dhyèvre & Serge Muller

  2. RITTMO Agro-Environnement, ZA Biopôle, 37, Rue de Herrlisheim, Colmar, 68025, France

    Pascale Chenon

  3. INERIS, Parc technologique Alata, BP2 Verneuil-en-Halatte, 60550, France

    Nicolas Manier & Pascal Pandard

  4. WELIENCE Agro-Environnement, RD 31, Bretenière, 21110, France

    Abdelwahad Echairi

  5. Université de Toulouse; INP, UPS, EcoLab (Laboratoire d’Ecologie Fonctionnelle et Environnement ENSAT, Avenue de l’Agrobiopole, Castanet Tolosan, 31326, France

    Jérôme Silvestre, Maritxu Guiresse & Eric Pinelli

  6. CNRS EcoLab, Castanet Tolosan, 31326, France

    Jérôme Silvestre, Maritxu Guiresse & Eric Pinelli

  7. National Research Council (CNR), Institute of Biology and Agricultural Biotechnology (IBBA), Research Unit of Pisa, Pisa, Italy

    Lucia Giorgetti

  8. National Research Council (CNR), Institute of Ecosystem Study (ISE), Research Unit of Pisa, Pisa, Italy

    Meri Barbafieri

  9. UNIVALI-Universidade do Vale do Itajaí, Laboratório de Remediação Ambiental, Rua Uruguai, 458, Itajaí, SC, 88302-202, Brazil

    Valéria C. Silva, Fernanda Engel & Claudemir M. Radetski

Authors
  1. Sylvie Cotelle
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  2. Adrien Dhyèvre
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  3. Serge Muller
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  4. Pascale Chenon
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  5. Nicolas Manier
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  6. Pascal Pandard
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  7. Abdelwahad Echairi
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  8. Jérôme Silvestre
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  9. Maritxu Guiresse
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  10. Eric Pinelli
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  11. Lucia Giorgetti
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  12. Meri Barbafieri
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  13. Valéria C. Silva
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  14. Fernanda Engel
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  15. Claudemir M. Radetski
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Corresponding authors

Correspondence to Sylvie Cotelle or Claudemir M. Radetski.

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Responsible editor: Philippe Garrigues

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Cotelle, S., Dhyèvre, A., Muller, S. et al. Soil genotoxicity assessment—results of an interlaboratory study on the Vicia micronucleus assay in the context of ISO standardization. Environ Sci Pollut Res 22, 988–995 (2015). https://doi.org/10.1007/s11356-014-3495-2

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  • DOI: https://doi.org/10.1007/s11356-014-3495-2

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