Skip to main content

Advertisement

SpringerLink
Log in

1H-NMR-based profiling of organic components in leachate from animal carcasses disposal site with time

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Leachate, generated by the decomposition of animal carcasses, presents many environmental, sanitary, and food safety hazards. However, research on the characteristics of leachate is lacking. In this study, we performed biochemical profiling of leachate from two animal species (pig and cattle) in two soil types (sandy loam and sandy soil) using 1H-NMR-based profiling, followed by multivariate data analysis. The leachate was collected from a well-controlled artificial burial site over a 31-week period. Principal components analysis (PCA) of the NMR data showed similar patterns between species and soil types. Organic components, including organic acids and phenols, predominated, and their levels increased with time. The methylamine level in leachate from pig carcasses 18 weeks following burial was significantly higher than that from cattle carcasses; leachate from cattle carcasses in sandy soil 1 week after burial contained unique components (specifically ethanol, formate, alanine, N-methylation, and taurine), in contrast with those from sandy loam soil. This study suggests that a NMR-based profiling approach is useful to characterize the organic components in leachate from animal carcasses over time.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Alexander DJ (2000) A review of avian influenza in different bird species. Vet Microbiol 74:3–13

    Article  CAS  Google Scholar 

  • Beigel JH, Farrar J, Han A, Hayden F, Hyer R, De Jong M, Lochindarat S, Nguyen T, Nguyen T, Tran T (2005) Avian influenza A (H5N1) infection in humans. New Engl J Med 353:1374–1385

    Article  Google Scholar 

  • Chan PK (2002) Outbreak of avian influenza A (H5N1) virus infection in Hong Kong in 1997. Clin Infect Dis 34:S58–S64

    Article  Google Scholar 

  • Davies G (2002) Foot and mouth disease. Res Vet Sci 73:195–199

    Article  Google Scholar 

  • Flory GA, Peer RW, Bendfeldt ES (2006). Evaluation of poultry carcass disposal methods used during an avian influenza outbreak in Virginia in 2002. Virginia Department of Environmental Quality

  • Glanville TD, Ahn H, Richard TL, Harmon JD, Reynolds DL, Akinc S (2006). Environmental impacts of emergency livestock mortality composting—leachate release and soil contamination. ASABES Annual International Meeting ASABE paper NO. 064049

  • Glanville TD, Ahn H, Richard T, Shiers L, Harmon JD (2009) Soil contamination caused by emergency bio-reduction of catastrophic livestock mortalities. Water Air Soil Pollut 198:285–295

    Article  CAS  Google Scholar 

  • Graiver DA, Topliff CL, Kelling CL, Bartelt-Hunt SL (2009) Survival of the avian influenza virus (H6N2) after land disposal. Environ Sci Technol 43:4063–4067

    Article  CAS  Google Scholar 

  • Grubman MJ, Baxt B (2004) Foot-and-mouth disease. Clin Microbiol Rev 17:465–493

    Article  CAS  Google Scholar 

  • Guan J, Chan M, Grenier C, Brooks B, Spencer J, Kranendonk C, Copps J, Clavijo A (2010) Degradation of foot-and-mouth disease virus during composting of infected pig carcasses. Can J Vet Res 74:40–44

    CAS  Google Scholar 

  • Kang M, Kim M-S, Choi B, Sohn H-Y (2012) Organic matter analysis and physicochemical properties of leachate from a foot-and-mouth disease landfill site. Korean J Microbiol Biotechnol 40:128–134

    Article  CAS  Google Scholar 

  • Kieny M, Lathe R, Drillien R, Spehner D, Skory S, Schmitt D, Wiktor T, Koprowski H, Lecocq J (1984) Expression of rabies virus glycoprotein from a recombinant vaccinia virus. Nature 312:163–166

    Article  CAS  Google Scholar 

  • Kimberlin RH, Wilesmith JW (1994) Bovine spongiform encephalopathy. Ann N Y Acad Sci 724:210–220

    Article  CAS  Google Scholar 

  • Maher D, Chaulet P, Spinaci S, Harries A (1997). Treatment of tuberculosis: guidelines for national programmes. Treatment of tuberculosis: guidelines for national programmes. Geneva World Health Organization 1-77

  • Martin FPJ, Wang Y, Sprenger N, Yap IK, Lundstedt T, Lek P, Rezzi S, Ramadan Z, van Bladeren P, Fay LB (2008) Probiotic modulation of symbiotic gut microbial–host metabolic interactions in a humanized microbiome mouse model. Mol Syst Biol 4:157

    Google Scholar 

  • McGee D (1977) Black leg (Leptosphaeria maculans (Desm.) Ces. de Not.) of rapeseed in Victoria: sources of infection and relationships between inoculum, environmental factors and disease severity. Crop Pasture Sci 28:53–62

    Article  Google Scholar 

  • Moennig V (2000) Introduction to classical swine fever: virus, disease and control policy. Vet Microbiol 73:93–102

    Article  CAS  Google Scholar 

  • Pratt D, Fonstad T (2009). Livestock mortalities burial leachate chemistry after two years of decomposition, Proceedings of 3rd International Symposium on Management of Animal Carcasses, Tissue and Related Byproducts

  • Reuter T, Xu W, Alexander TW, Gilroyed BH, Inglis GD, Larney FJ, Stanford K, McAllister TA (2010) Biocontained carcass composting for control of infectious disease outbreak in livestock. J Vis Exp 39:e1946

    Google Scholar 

  • Smith E, Macfarlane G (1997) Dissimilatory amino acid metabolism in human colonic bacteria. Anaerobe 3:327–337

    Article  CAS  Google Scholar 

  • Swayne D, Suarez D (2000) Highly pathogenic avian influenza. Revue scientifique et technique. Rev Sci Tech 19:463–482

    CAS  Google Scholar 

  • Thauer RK (1998) Biochemistry of methanogenesis: a tribute to Marjory Stephenson: 1998 Marjory Stephenson Prize Lecture. Microbiol 144:2377–2406

    Article  CAS  Google Scholar 

  • Topping DL, Clifton PM (2001) Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev 81:1031–1064

    CAS  Google Scholar 

  • Varel VH (2002) Carvacrol and thymol reduce swine waste odor and pathogens: stability of oils. Curr Microbiol 44:38–43

    Article  CAS  Google Scholar 

  • Warner MA, Harper JV (1985) Cardiac dysrhythmias associated with chemical peeling with phenol. Anesthesiology 62:366–367

    Article  CAS  Google Scholar 

  • Yoo HS (2011) Foot and mouth disease: etiology, epidemiology and control measures. Infect Chemother 43:178–185

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by a grant from the Korea Basic Science Institute (C34705) and the National Research Foundation of Korea Grant funded by the Korean Government (MSIP) (2013, University-Institute cooperation program).

Author information

Authors and Affiliations

  1. Western Seoul Center, Korea Basic Science Institute, 150 Bugahyeon-ro, Seodamun-gu, Seoul, 120-140, Republic of Korea

    Yong-Kook Kwon, Hyun-Whee Bae, Jungju Seo & Geum-Sook Hwang

  2. Graduate School of Analytical Science and Technology, Chungnam University, Daejeon, Republic of Korea

    Hyun-Whee Bae & Geum-Sook Hwang

  3. National Institute of Environmental Research, Incheon, Republic of Korea

    Sun Kyoung Shin & Tae-Wan Jeon

Authors
  1. Yong-Kook Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyun-Whee Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sun Kyoung Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tae-Wan Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jungju Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Geum-Sook Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Geum-Sook Hwang.

Additional information

Responsible editor: Philippe Garrigues

Yong-Kook Kwon and Hyun-Whee Bae contributed equally to the work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kwon, YK., Bae, HW., Shin, S.K. et al. 1H-NMR-based profiling of organic components in leachate from animal carcasses disposal site with time. Environ Sci Pollut Res 21, 10453–10460 (2014). https://doi.org/10.1007/s11356-014-2992-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-014-2992-7

Keywords

Search

Navigation