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A Hyaluronic Acid-Conjugated Gadolinium Hepatocyte-Specific T1 Contrast Agent for Liver Magnetic Resonance Imaging

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Abstract

Purpose

In this study, we synthesized hyaluronic acid-conjugated gadolinium (HA-diethylene triamine pentaacetic acid (DTPA)-Gd) and evaluated as hepatocyte-specific magnetic resonance imaging (MRI) contrast agent for the diagnosis of hepatic metastasis.

Procedures

We conducted Fourier transform (FT)-IR analysis to determine the conjugation of HA and DTPA and performed cell viability assays using NIH3T3 and FL83B cell lines. We also conducted T1-weighted MRI of HA-DTPA-Gd and gadoxetic acid to compare the paramagnetic properties of both.

Results

HA-DTPA-Gd had a higher efficiency in liver MRI compared with the commercially available liver-specific contrast agent (p < 0.001). HA-DTPA-Gd, which possessed a higher T1 relaxivity, showed excellent capability for the diagnosis of hepatic metastasis through an in vivo MRI study in comparison with gadoxetic acid (p < 0.001).

Conclusion

Based on this study, we believe that HA-DTPA-Gd has promising potential for use as a contrast agent for liver MRI of hepatic metastases.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2011–0023317)

Conflict of Interest

The authors declare that they have no conflict of interest.

Author Contributions

The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Authors and Affiliations

  1. Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 501-746, South Korea

    MyeongJu Moon, Reju George Thomas, Seon-u Heo, Suk Hee Heo, Nam Yeol Yim & Yong Yeon Jeong

  2. Department of Internal Medicine, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 501-746, South Korea

    Myong-Suk Park & Woo Kyun Bae

Authors
  1. MyeongJu Moon
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  2. Reju George Thomas
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  3. Seon-u Heo
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  4. Myong-Suk Park
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  5. Woo Kyun Bae
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  6. Suk Hee Heo
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  7. Nam Yeol Yim
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  8. Yong Yeon Jeong
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Correspondence to Yong Yeon Jeong.

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Moon, M., Thomas, R.G., Heo, Su. et al. A Hyaluronic Acid-Conjugated Gadolinium Hepatocyte-Specific T1 Contrast Agent for Liver Magnetic Resonance Imaging. Mol Imaging Biol 17, 497–503 (2015). https://doi.org/10.1007/s11307-014-0819-z

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  • DOI: https://doi.org/10.1007/s11307-014-0819-z

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