Research Article

Molecular Imaging and Biology

, Volume 16, Issue 2, pp 235-245

Comparison of 4D-MicroSPECT and MicroCT for Murine Cardiac Function

  • Nicholas T. BeferaAffiliated withCenter for In Vivo Microscopy, Department of Radiology, Duke University Medical Center
  • , Cristian T. BadeaAffiliated withCenter for In Vivo Microscopy, Department of Radiology, Duke University Medical Center
  • , G. Allan JohnsonAffiliated withCenter for In Vivo Microscopy, Department of Radiology, Duke University Medical Center Email author 

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Abstract

Purpose

The objective of this study was to compare a new generation of four-dimensional micro-single photon emission computed tomography (microSPECT) with microCT for the quantitative in vivo assessment of murine cardiac function.

Procedures

Four-dimensional isotropic cardiac images were acquired from anesthetized normal C57BL/6 mice with either microSPECT (n = 6) or microCT (n = 6). One additional mouse with myocardial infarction (MI) was scanned with both modalities. Prior to imaging, mice were injected with either technetium tetrofosmin for microSPECT or a liposomal blood pool contrast agent for microCT. Segmentation of the left ventricle (LV) was performed using Vitrea (Vital Images) software, to derive global and regional function.

Results

Measures of global LV function between microSPECT and microCT groups were comparable (e.g., ejection fraction = 71 ± 6 % microSPECT and 68 ± 4 % microCT). Regional functional indices (wall motion, wall thickening, regional ejection fraction) were also similar for the two modalities. In the mouse with MI, microSPECT identified a large perfusion defect that was not evident with microCT.

Conclusions

Despite lower spatial resolution, microSPECT was comparable to microCT in the quantitative evaluation of cardiac function. MicroSPECT offers an advantage over microCT in the ability to evaluate simultaneously myocardial radiotracer distribution and function, simultaneously. MicroSPECT should be considered as an alternative to microCT and magnetic resonance for preclinical cardiac imaging in the mouse.

Key words

Murine cardiac functional imaging Molecular imaging MicroSPECT MicroCT Dynamic microSPECT Micro-CT Four-dimensional (4D) cardiac imaging 99mTc tetrofosmin Mice Cardiovascular imaging