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Molecular Imaging of Neuroblastoma Progression in TH-MYCN Transgenic Mice

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Abstract

Purpose

TH-MYCN transgenic mice represent a valuable preclinical model of neuroblastoma. Current methods to study tumor progression in these mice are inaccurate or invasive, limiting the potential of this murine model. The aim of our study was to assess the potential of small animal positron emission tomography (SA-PET) to study neuroblastoma progression in TH-MYCN mice.

Procedure

Serial SA-PET scans using the tracer 2-deoxy-2-[18F]fluoro-d-glucose (18F-FDG) have been performed in TH-MYCN mice. Image analysis of tumor progression has been compared with ex vivo evaluation of tumor volumes and histological features.

Results

[18F]FDG-SA-PET allowed to detect early staged tumors in almost 100 % of TH-MYCN mice positive for disease. Image analysis of tumor evolution reflected the modifications of the tumor volume, histological features, and malignancy during disease progression. Image analysis of TH-MYCN mice undergoing chemotherapy treatment against neuroblastoma provided information on drug-induced alterations in tumor metabolic activity.

Conclusions

These data show for the first time that [18F]FDG-SA-PET is a useful tool to study neuroblastoma presence and progression in TH-MYCN transgenic mice.

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Acknowledgments

We thank Dr. Pasquale Chieco and the Center for Applied Biomedical Research (CRBA), S. Orsola-Malpighi University Hospital, Via Massarenti 9, 40138 Bologna, Italy for support and expert assistance with histology and immunohistochemistry. We thank Prof. Hein te Riele for editorial and scientific suggestions. We thank the Italian Foundation for Neuroblastoma Research, AIRC, AGEOP, and NIH R01CA102321 for support.

Conflict of Interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Nuclear Medicine, S. Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy

    Carmelo Quarta, Cristina Nanni, Valentina Ambrosini, Daniela D’ambrosio, Federico Zagni, Filippo Lodi, Mario Marengo & Stefano Fanti

  2. Pediatric Hematology–Oncology, University of Bologna, Bologna, Italy

    Erika Cantelli, Korinne Di Leo & Andrea Pession

  3. Department of Medical Physics, S.Orsola-Malpighi Hospital, Bologna, Italy

    Daniela D’ambrosio, Federico Zagni & Mario Marengo

  4. University of California San Francisco, San Francisco, CA, USA

    William A. Weiss

  5. Department of Pharmacology, University of Bologna, Bologna, Italy

    Silvia Angelucci & Roberto Tonelli

Authors
  1. Carmelo Quarta
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  2. Erika Cantelli
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  3. Cristina Nanni
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  6. Korinne Di Leo
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  7. Silvia Angelucci
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  8. Federico Zagni
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  9. Filippo Lodi
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  10. Mario Marengo
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  11. William A. Weiss
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  12. Andrea Pession
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  13. Roberto Tonelli
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  14. Stefano Fanti
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Corresponding author

Correspondence to Carmelo Quarta.

Additional information

Carmelo Quarta, Erika Cantelli, Roberto Tonelli, and Stefano Fanti contributed equally to this work.

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Quarta, C., Cantelli, E., Nanni, C. et al. Molecular Imaging of Neuroblastoma Progression in TH-MYCN Transgenic Mice. Mol Imaging Biol 15, 194–202 (2013). https://doi.org/10.1007/s11307-012-0576-9

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  • DOI: https://doi.org/10.1007/s11307-012-0576-9

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