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Impact of Indium-111 Oxine Labelling on Viability of Human Mesenchymal Stem Cells In Vitro, and 3D Cell-Tracking Using SPECT/CT In Vivo

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Abstract

Purpose

This study investigates the effects of 111In-oxine incorporation on human mesenchymal stem cells’ (hMSC) biology and viability, and the applicability of 111In-oxine for single-photon emission computed tomography/X-ray computed tomography (SPECT/CT) monitoring of hMSC in vivo.

Procedures

HMSC were labelled with 10 Bq/cell. Cellular retention of radioactivity, cell survival, and migration were evaluated over 48 h. Metabolic activity was assessed over 14 days and the hMSC’s stem cell character was evaluated. Serial SPECT/CT was performed after intra-osseous injection to athymic rats over 48 h.

Results

Labelling efficiency was 25%, with 61% of incorporated 111In remaining in the hMSC at 48 h. The radiolabelling was without effect on cell viability, stem cell character, and plasticity, whereas metabolic activity and migration were significantly reduced. Grafted cells could be imaged in situ with SPECT/CT.

Conclusions

111In-oxine labelling moderately impaired hMSC’s functional integrity while preserving their stem cell character. Combined SPECT/CT imaging of 111In-oxine-labelled hMSC opens the possibility for non-invasive sequential monitoring of therapeutic stem cells.

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Acknowledgements

This work was supported by a research grant from the Bavarian Research Foundation (Bavarian Research Collaboration for Cell-Based Regeneration of the Musculo-Skeletal System in Old Age, www.forzebra.de). We thank Mr. S. Nowak, Ms. M. Buchwald, Ms. T. Bockhöfer, and Ms. A. Brunegraf for assistance in SPECT/CT image acquisition.

Conflicts of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Experimental Surgery and Regenerative Medicine, Department of Surgery, University of Munich (LMU), Nussbaumstr. 20, 80336, Munich, Germany

    Florian Haasters, Inga Drosse, Wolf Mutschler & Matthias Schieker

  2. Department of Nuclear Medicine, University of Munich (LMU), Marchioninistraße 15, 81377, Munich, Germany

    Franz Josef Gildehaus, Erika Wagner, Christian Zach, Paul Cumming & Peter Bartenstein

Authors
  1. Franz Josef Gildehaus
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  2. Florian Haasters
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  3. Inga Drosse
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  7. Paul Cumming
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  9. Matthias Schieker
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Corresponding author

Correspondence to Matthias Schieker.

Additional information

Franz Josef Gildehaus and Florian Haasters contributed equally to this work

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Gildehaus, F.J., Haasters, F., Drosse, I. et al. Impact of Indium-111 Oxine Labelling on Viability of Human Mesenchymal Stem Cells In Vitro, and 3D Cell-Tracking Using SPECT/CT In Vivo . Mol Imaging Biol 13, 1204–1214 (2011). https://doi.org/10.1007/s11307-010-0439-1

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