Molecular Imaging and Biology

, Volume 12, Issue 5, pp 463–467

AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging

  • Bulent Aydogan
  • Ji Li
  • Tijana Rajh
  • Ahmed Chaudhary
  • Steven J. Chmura
  • Charles Pelizzari
  • Christian Wietholt
  • Metin Kurtoglu
  • Peter Redmond
Rapid Communication

DOI: 10.1007/s11307-010-0299-8

Cite this article as:
Aydogan, B., Li, J., Rajh, T. et al. Mol Imaging Biol (2010) 12: 463. doi:10.1007/s11307-010-0299-8

Abstract

Purpose

To study the feasibility of using 2-deoxy-d-glucose (2-DG)-labeled gold nanoparticle (AuNP-DG) as a computed tomography (CT) contrast agent with tumor targeting capability through in vitro experiments.

Procedures

Gold nanoparticles (AuNP) were fabricated and were conjugated with 2-deoxy-d-glucose. The human alveolar epithelial cancer cell line, A-549, was chosen for the in vitro cellular uptake assay. Two groups of cell samples were incubated with the AuNP-DG and the unlabeled AuNP, respectively. Following the incubation, the cells were washed with sterile PBS to remove the excess gold nanoparticles and spun to cell pellets using a centrifuge. The cell pellets were imaged using a microCT scanner immediately after the centrifugation. The reconstructed CT images were analyzed using a commercial software package.

Results

Significant contrast enhancement in the cell samples incubated with the AuNP-DG with respect to the cell samples incubated with the unlabeled AuNP was observed in multiple CT slices.

Conclusions

Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.

Key words

Gold nanoparticleContrast-enhanced CT2-Deoxy-d-glucoseCancer imagingTumor targeting

Copyright information

© Academy of Molecular Imaging and Society for Molecular Imaging 2010

Authors and Affiliations

  • Bulent Aydogan
    • 1
  • Ji Li
    • 1
  • Tijana Rajh
    • 2
  • Ahmed Chaudhary
    • 1
  • Steven J. Chmura
    • 1
  • Charles Pelizzari
    • 1
  • Christian Wietholt
    • 3
    • 4
  • Metin Kurtoglu
    • 5
  • Peter Redmond
    • 2
  1. 1.Department of Radiation and Cellular OncologyThe University of ChicagoChicagoUSA
  2. 2.Center for Nanoscale MaterialsArgonne National LaboratoryArgonneUSA
  3. 3.Department of RadiologyThe University of ChicagoChicagoUSA
  4. 4.Department of Medicine/CardiologyThe University of ChicagoChicagoUSA
  5. 5.Department of Hematology and Medical OncologyEmory University Winship Cancer InstituteAtlantaUSA