AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging
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To study the feasibility of using 2-deoxy-d-glucose (2-DG)-labeled gold nanoparticle (AuNP-DG) as a computed tomography (CT) contrast agent with tumor targeting capability through in vitro experiments.
Gold nanoparticles (AuNP) were fabricated and were conjugated with 2-deoxy-d-glucose. The human alveolar epithelial cancer cell line, A-549, was chosen for the in vitro cellular uptake assay. Two groups of cell samples were incubated with the AuNP-DG and the unlabeled AuNP, respectively. Following the incubation, the cells were washed with sterile PBS to remove the excess gold nanoparticles and spun to cell pellets using a centrifuge. The cell pellets were imaged using a microCT scanner immediately after the centrifugation. The reconstructed CT images were analyzed using a commercial software package.
Significant contrast enhancement in the cell samples incubated with the AuNP-DG with respect to the cell samples incubated with the unlabeled AuNP was observed in multiple CT slices.
Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.
- AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging
Molecular Imaging and Biology
Volume 12, Issue 5 , pp 463-467
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- Gold nanoparticle
- Contrast-enhanced CT
- Cancer imaging
- Tumor targeting
- Industry Sectors
- Author Affiliations
- 1. Department of Radiation and Cellular Oncology, The University of Chicago, Chicago, IL, USA
- 2. Center for Nanoscale Materials, Argonne National Laboratory, Argonne, IL, USA
- 3. Department of Radiology, The University of Chicago, Chicago, IL, USA
- 4. Department of Medicine/Cardiology, The University of Chicago, Chicago, IL, USA
- 5. Department of Hematology and Medical Oncology, Emory University Winship Cancer Institute, Atlanta, GA, USA