Rapid Communication

Molecular Imaging and Biology

, Volume 12, Issue 5, pp 463-467

AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging

  • Bulent AydoganAffiliated withDepartment of Radiation and Cellular Oncology, The University of Chicago Email author 
  • , Ji LiAffiliated withDepartment of Radiation and Cellular Oncology, The University of Chicago
  • , Tijana RajhAffiliated withCenter for Nanoscale Materials, Argonne National Laboratory
  • , Ahmed ChaudharyAffiliated withDepartment of Radiation and Cellular Oncology, The University of Chicago
  • , Steven J. ChmuraAffiliated withDepartment of Radiation and Cellular Oncology, The University of Chicago
  • , Charles PelizzariAffiliated withDepartment of Radiation and Cellular Oncology, The University of Chicago
  • , Christian WietholtAffiliated withDepartment of Radiology, The University of ChicagoDepartment of Medicine/Cardiology, The University of Chicago
  • , Metin KurtogluAffiliated withDepartment of Hematology and Medical Oncology, Emory University Winship Cancer Institute
  • , Peter RedmondAffiliated withCenter for Nanoscale Materials, Argonne National Laboratory

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Abstract

Purpose

To study the feasibility of using 2-deoxy-d-glucose (2-DG)-labeled gold nanoparticle (AuNP-DG) as a computed tomography (CT) contrast agent with tumor targeting capability through in vitro experiments.

Procedures

Gold nanoparticles (AuNP) were fabricated and were conjugated with 2-deoxy-d-glucose. The human alveolar epithelial cancer cell line, A-549, was chosen for the in vitro cellular uptake assay. Two groups of cell samples were incubated with the AuNP-DG and the unlabeled AuNP, respectively. Following the incubation, the cells were washed with sterile PBS to remove the excess gold nanoparticles and spun to cell pellets using a centrifuge. The cell pellets were imaged using a microCT scanner immediately after the centrifugation. The reconstructed CT images were analyzed using a commercial software package.

Results

Significant contrast enhancement in the cell samples incubated with the AuNP-DG with respect to the cell samples incubated with the unlabeled AuNP was observed in multiple CT slices.

Conclusions

Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.

Key words

Gold nanoparticle Contrast-enhanced CT 2-Deoxy-d-glucose Cancer imaging Tumor targeting