Molecular Imaging and Biology

, Volume 11, Issue 1, pp 31–38

An In Vivo Multimodal Imaging Study Using MRI and PET of Stem Cell Transplantation after Myocardial Infarction in Rats

Authors

  • Catherine Chapon
    • Stem Cell ImagingMRC Clinical Sciences Centre, Imperial College London
  • Johanna S. Jackson
    • Stem Cell ImagingMRC Clinical Sciences Centre, Imperial College London
  • Eric O. Aboagye
    • Molecular TherapyMRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital
  • Amy H. Herlihy
    • Biological Imaging Centre, Imaging Science DepartmentMRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital
  • William A. Jones
    • Stem Cell ImagingMRC Clinical Sciences Centre, Imperial College London
    • Stem Cell ImagingMRC Clinical Sciences Centre, Imperial College London
Research Article

DOI: 10.1007/s11307-008-0174-z

Cite this article as:
Chapon, C., Jackson, J.S., Aboagye, E.O. et al. Mol Imaging Biol (2009) 11: 31. doi:10.1007/s11307-008-0174-z

Abstract

Purpose

The purpose of the study is to track iron-oxide nanoparticle-labelled adult rat bone marrow-derived stem cells (IO-rBMSCs) by magnetic resonance imaging (MRI) and determine their effect in host cardiac tissue using 2-deoxy-2-[F-18]fluoro-d-glucose-positron emission tomography (FDG-PET).

Procedures

Infarcted rats were randomised to receive (1) live IO-rBMSCs by direct local injection, or (2) dead IO-rBMSCs as controls; (3) sham-operated rats received live IO-rBMSCs. The rats were then imaged from 2 days to 6 weeks post-cell implantation using both MRI at 9.4T and FDG-PET.

Results

Implanted IO-rBMSCs were visible in the heart by MRI for the duration of the study. Histological analysis confirmed that the implanted IO-rBMSCs were present for up to 6 weeks post-implantation. At 1 week post-IO-rBMSC transplantation, PET studies demonstrated an increase in FDG uptake in infarcted regions implanted with live IO-rBMSC compared to controls.

Conclusions

Noninvasive multimodality imaging allowed us to visualise IO-rBMSCs and establish their affect on cardiac function in a rat model of myocardial infarction (MI).

Key words

Myocardial infarctionMRIPETStem cellRat

Copyright information

© Academy of Molecular Imaging 2008