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An In Vivo Multimodal Imaging Study Using MRI and PET of Stem Cell Transplantation after Myocardial Infarction in Rats

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Abstract

Purpose

The purpose of the study is to track iron-oxide nanoparticle-labelled adult rat bone marrow-derived stem cells (IO-rBMSCs) by magnetic resonance imaging (MRI) and determine their effect in host cardiac tissue using 2-deoxy-2-[F-18]fluoro-d-glucose-positron emission tomography (FDG-PET).

Procedures

Infarcted rats were randomised to receive (1) live IO-rBMSCs by direct local injection, or (2) dead IO-rBMSCs as controls; (3) sham-operated rats received live IO-rBMSCs. The rats were then imaged from 2 days to 6 weeks post-cell implantation using both MRI at 9.4T and FDG-PET.

Results

Implanted IO-rBMSCs were visible in the heart by MRI for the duration of the study. Histological analysis confirmed that the implanted IO-rBMSCs were present for up to 6 weeks post-implantation. At 1 week post-IO-rBMSC transplantation, PET studies demonstrated an increase in FDG uptake in infarcted regions implanted with live IO-rBMSC compared to controls.

Conclusions

Noninvasive multimodality imaging allowed us to visualise IO-rBMSCs and establish their affect on cardiac function in a rat model of myocardial infarction (MI).

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Acknowledgements

The authors would like to thank the Nuclear Medicine Service of Hammersmith Hospital, London UK, for the kind gift of FDG. Thanks to FMRIB at Oxford University for the original pulse sequences.

Conflict of Interest

The authors declare that there is no conflict of interest.

Funding

This work was funded by the Medical Research Council (MRC) of Great Britain. EOA’s laboratory is funded by Cancer Research-UK and MRC.

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Authors and Affiliations

  1. Stem Cell Imaging, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Campus, Du Cane Road, London, W12 0NN, UK

    Catherine Chapon, Johanna S. Jackson, William A. Jones & Kishore K. Bhakoo

  2. Molecular Therapy, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, London, UK

    Eric O. Aboagye

  3. Biological Imaging Centre, Imaging Science Department, MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital, London, UK

    Amy H. Herlihy

Authors
  1. Catherine Chapon
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  2. Johanna S. Jackson
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  3. Eric O. Aboagye
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  4. Amy H. Herlihy
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  5. William A. Jones
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  6. Kishore K. Bhakoo
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Corresponding author

Correspondence to Kishore K. Bhakoo.

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Chapon, C., Jackson, J.S., Aboagye, E.O. et al. An In Vivo Multimodal Imaging Study Using MRI and PET of Stem Cell Transplantation after Myocardial Infarction in Rats. Mol Imaging Biol 11, 31–38 (2009). https://doi.org/10.1007/s11307-008-0174-z

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  • DOI: https://doi.org/10.1007/s11307-008-0174-z

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