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Assessment of freezing effects and diagnostic potential of BioBank healthy and neoplastic breast tissues through HR-MAS NMR spectroscopy

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Abstract

HR-MAS NMR spectroscopy was employed to monitor the metabolic profiles of Modena BioBank breast samples over 1 year of freezing at −80 °C. The study includes 22 adult female patients living in Modena and its hinterland, who underwent total mastectomy or quadrantectomy in 2011–2012. Variations occur, especially affecting phosphocholine (PC) and choline. This is not a trivial finding, since many studies base the distinction between neoplastic and healthy tissues or the assessment of tumor grade on the analysis of choline metabolites. Despite the changes observed, we established that the diagnostic power of the HR-MAS NMR spectra of frozen samples is preserved, at least as far as the distinction between neoplastic and healthy samples is concerned. Lactate (Lac), PC, phosphoethanolamine (PE), taurine (Tau), myo-inositol (Myo) and glucose (Glc) are biomarkers that can be used to distinguish healthy from neoplastic tissues, whereas some metabolite ratios, such as Lac + PE + Tau/Glc + Myo, seem to have even higher discrimination potential.

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Abbreviations

Asc:

Ascorbate

TGgl :

Bonded glycerol

CPMG:

Carr–Purcell–Meiboom–Gill

Cho:

Choline

ChoCC:

Choline containing compounds

E:

Ethanolamine

Glc:

Glucose

GPC:

Glycerophosphocholine

Gly:

Glycine

HR-MAS:

High-Resolution Magic Angle Spinning

Lac:

Lactate

Myo:

Myo-inositol

NMR:

Nuclear Magnetic Resonance

PLS-DA:

Partial Least Squares-Discriminant Analysis

PC:

Phosphocholine

PE:

Phosphoethanolamine

PCA:

Principal Component Analysis

ROCCET:

Receiver Operating Characteristic Curve Explorer & Tester

Tau:

Taurine

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Authors and Affiliations

  1. Dipartimento di Scienze per la Qualità della Vita, Università di Bologna, C.so D’Augusto 237, 47921, Rimini, Italy

    Valeria Righi

  2. Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, Via G. Campi 183, 41125, Modena, Italy

    Luisa Schenetti

  3. Dipartimento di Medicina Diagnostica, Clinica e di Sanità Pubblica, Università di Modena e Reggio Emilia, Policlinico, Via del Pozzo, 71, 41125, Modena, Italy

    Antonino Maiorana, Stefania Bettelli & Luca Reggiani Bonetti

  4. Dipartimento di Scienze Chimiche e Geologiche, Università di Modena e Reggio Emilia, Via G. Campi 183, 41125, Modena, Italy

    Emanuela Libertini & Adele Mucci

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  1. Valeria Righi
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Correspondence to Adele Mucci.

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Righi, V., Schenetti, L., Maiorana, A. et al. Assessment of freezing effects and diagnostic potential of BioBank healthy and neoplastic breast tissues through HR-MAS NMR spectroscopy. Metabolomics 11, 487–498 (2015). https://doi.org/10.1007/s11306-014-0709-z

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