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Construction of a high density linkage map and its application in the identification of QTLs for soluble sugar and organic acid components in apple

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Abstract

Soluble sugars and organic acids have a strong impact on the overall organoleptic quality of fruits. In this study, we report the identification of quantitative trait loci (QTLs) for individual sugars and organic acids in apple. A high density linkage map of apple was constructed using the 1536 EST-derived SNP GoldenGate genotyping platform. The linkage map consists of 601 molecular markers, including 540 single nucleotide polymorphisms (SNPs) and 61 simple sequence repeats (SSRs), spanning 1368.4 cM with an average of 2.28 cM per marker. The contents of soluble sugars, including sucrose, glucose, fructose, sorbitol, and organic acids, including malic acid and citric acid, were used as the phenotypic data in QTL analysis. Two QTLs for malic acid content were detected on linkage groups (LGs) 8 and 16, while no QTL was found for citric acid content. Four QTLs for the glucose, sucrose, fructose, and sorbitol content were found to be clustered in one region on LG 3. Moreover, an additional QTL for glucose content was detected on the LG 4. Our study not only expands our understanding of the genetic basis for fruit organoleptic quality but it also provides molecular markers that will aid in marker-assisted selection for fruit quality in apple breeding programs.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31420103914 and 31372048), the National Basic Research Program of China (Grant No. 2011CB100600), and the National High Technology Research and Development Program of China (Grant No. 2011AA0020401).

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Authors and Affiliations

  1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden of the Chinese Academy of Sciences, Wuhan, 430074, China

    Baiquan Ma, Shuang Zhao, Qian Peng, Albert Owiti, Ting Fang, Liao Liao, Collins Ogutu & Yuepeng Han

  2. Beijing Key Laboratory of Grape Sciences and Enology, and CAS Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Benhong Wu & Shaohua Li

  3. Graduate University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing, 100049, China

    Baiquan Ma, Shuang Zhao, Qian Peng, Albert Owiti, Ting Fang & Collins Ogutu

  4. Liaoning Province Institute of Pomology, Xiongyue, Liaoning Province, 115214, China

    Dongmei Wang

  5. Department of Biology, University of Massachusetts Boston, Boston, MA, 02184, USA

    Schuyler S. Korban

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  1. Baiquan Ma
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  2. Shuang Zhao
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  3. Benhong Wu
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  4. Dongmei Wang
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  10. Schuyler S. Korban
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  11. Shaohua Li
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  12. Yuepeng Han
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Corresponding authors

Correspondence to Shaohua Li or Yuepeng Han.

Additional information

Communicated by M. Troggio

Data archiving statement

The consensus map of the JG×WSH mapping population is currently being prepared for submitting to Genome Database for Rosaceae (https://www.rosaceae.org).

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Ma, B., Zhao, S., Wu, B. et al. Construction of a high density linkage map and its application in the identification of QTLs for soluble sugar and organic acid components in apple. Tree Genetics & Genomes 12, 1 (2016). https://doi.org/10.1007/s11295-015-0959-6

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