Tree Genetics & Genomes

, Volume 9, Issue 4, pp 1031–1041

Transcriptome characterization and detection of gene expression differences in aspen (Populus tremuloides)

  • Hardeep S. Rai
  • Karen E. Mock
  • Bryce A. Richardson
  • Richard C. Cronn
  • Katherine J. Hayden
  • Jessica W. Wright
  • Brian J. Knaus
  • Paul G. Wolf
Original Paper

DOI: 10.1007/s11295-013-0615-y

Cite this article as:
Rai, H.S., Mock, K.E., Richardson, B.A. et al. Tree Genetics & Genomes (2013) 9: 1031. doi:10.1007/s11295-013-0615-y

Abstract

Aspen (Populus tremuloides) is a temperate North American tree species with a geographical distribution more extensive than any other tree species on the continent. Because it is economically important for pulp and paper industries and ecologically important for its role as a foundation species in forest ecosystems, the decline of aspen in large portions of its range is of serious concern. The availability and annotation of the black cottonwood (Populus trichocarpa) genome enables a range of high throughput sequencing approaches that can be used to understand rangewide patterns of genetic variation, adaptation, and responses to environmental challenges in other Populus species, including aspen. Gene expression studies are particularly useful for understanding the molecular basis of ecological responses, but are limited by the availability of transcriptome data. We explored the aspen transcriptome through the use of high-throughput sequencing with two main goals: (1) characterization of the expressed portion of the P. tremuloides genome in leaves and (2) assessment of variation in gene expression among genets collected from distinct latitudes but reared in a common garden. We also report a large single nucleotide polymorphism dataset that provides the groundwork for future studies of aspen evolution and ecology, and we identify a set of differentially expressed genes across individuals and population boundaries for the leaf transcriptome of P. tremuloides.

Keywords

Trembling aspenQuaking aspenRNA-SeqDifferential expressionSNP (single nucleotide polymorphism)Populus trichocarpa

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2013

Authors and Affiliations

  • Hardeep S. Rai
    • 1
  • Karen E. Mock
    • 1
  • Bryce A. Richardson
    • 2
  • Richard C. Cronn
    • 3
  • Katherine J. Hayden
    • 4
  • Jessica W. Wright
    • 5
  • Brian J. Knaus
    • 3
  • Paul G. Wolf
    • 6
  1. 1.Ecology Center and Department of Wildland ResourcesUtah State UniversityLoganUSA
  2. 2.Rocky Mountain Research StationUSDA Forest ServiceProvoUSA
  3. 3.Pacific Northwest Research StationUSDA Forest ServiceCorvallisUSA
  4. 4.Department of Environmental Science, Policy, and ManagementUniversity of CaliforniaBerkeleyUSA
  5. 5.Pacific Southwest Research StationUSDA- Forest ServiceDavisUSA
  6. 6.Ecology Center and Department of BiologyUtah State UniversityLoganUSA