Skip to main content

Advertisement

SpringerLink
Log in

Inhibitory effect of a fermented soy product from lactic acid bacteria (PS-B1) on deterioration of bone mass and quality in ovariectomized mice

  • Original Article
  • Published:
Oral Radiology Aims and scope Submit manuscript

Abstract

Objectives

We investigated the effects of a fermented soy product from lactic acid bacteria (PS-B1) in preventing the decrease in bone mineral content and deterioration of trabecular bone structure in ovariectomized (OVX) mice.

Methods

Four-week-old ICR OVX or sham-operated (Sham) mice were maintained on a normal diet in five groups: a Sham group, the OVX control group, PS-B1 1 % (Ps 1 %) group, PS-B1 2 % (Ps 2 %) group, and PS-B1 5 % (Ps 5 %) group. The Sham and OVX control groups were given tap water as drinking water. Water containing 1, 2, and 5 % PS-B1 was given ad libitum to the respective PS-B1 groups. The animals were kept under these conditions for 12 weeks and sacrificed under anesthesia. Their femurs were removed under sterile conditions. Bone mineral density (BMD) of the removed femurs was measured by use of peripheral quantitative computed tomography. Subsequently, morphometric indices for trabecular bone structure were measured by use of microfocus X-ray computed tomography.

Results

Total BMD, trabecular BMD, and cortical width were significantly higher in the Ps 2 % group than in controls. In the Ps 2 % group, there was a tendency for retarded BMD loss. Maintenance of bone volume fraction, trabecular number, trabecular space, trabecular continuity, and trabecular connectivity were seen in the Ps 2 % and Ps 5 % groups. In the Ps 2 % and Ps 5 % groups, deterioration of trabecular bone structure was significantly inhibited.

Conclusions

These results suggest that PS-B1 is effective in slowing the decrease in BMD and deterioration of trabecular bone structure in OVX mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Tanaka M, Shikama R, Ishibashi G, Honda H, Ogasawara M, Sakakibara R. The value as the nutritional and serum cholesterol effects of the fermented products from milk whey (PS-H1) in rats. J Integr Stud Diet Habits. 2004;14:323–7.

    Article  Google Scholar 

  2. Matsumaru T, Tanaka M, Shikama R, Ishibashi G, Matsuno K, Honda H, et al. Effects of the fermented products from milk whey on defecation in humans. Bull Kyushu Women’s Univ. 2003;40:51–60.

    Google Scholar 

  3. Nodake Y, Ogasawara M, Honda H, Fukasawa M, Sakakibara R. Characterization and preliminary purification of the anti-cancer substance(s) in the fermented products cultivated from soybean milk using lactic acid bacteria. Saito Ho-on Kai Mus Nat Hist Res Bull. 2008;73:17–21.

    Google Scholar 

  4. The National Institutes of Health. Osteoporosis prevention, diagnosis, and therapy. JAMA. 2001;285:785–95.

    Article  Google Scholar 

  5. Kanis JA, Johnell O, DeLaet C, Jonsson B, Oden A, Ogelsby AK. International variations in hip fracture probabilities: implications for risk assessment. J Bone Miner Res. 2002;17:1237–44.

    Article  PubMed  Google Scholar 

  6. Aoki K, Saito H, Itzstein C, Ishiguro M, Shibata T, Blanque R, et al. A TNF receptor loop peptide mimic blocks RANK ligand-induced signaling, bone resorption, and bone loss. J Clin Invest. 2006;116:1525–34.

    Article  PubMed Central  PubMed  Google Scholar 

  7. Tsuchita H, Goto T, Shimizu T, Yonehara Y, Kuwata T. Dietary casein phosphopeptides prevent bone loss in aged OVX rats. J Nutr. 1996;126:86–93.

    PubMed  Google Scholar 

  8. Ma DF, Qin LQ, Wang PY, Katoh R. Soy isoflavone intake inhibits bone resorption and stimulates bone formation in menopausal women: meta-analysis of randomized controlled trials. Eur J Clin Nutr. 2007;62:155–61.

    Article  PubMed  Google Scholar 

  9. Lind PM, Lind L, Larsson S, Orberg J. Torsional testing and peripheral quantitative computed tomography in rat humerus. Bone. 2001;29:265–70.

    Article  PubMed  Google Scholar 

  10. Pothuaud L, Lespessailles E, Harba R, Jennane R, Royant V, Eynard E, et al. Fractal analysis of trabecular bone texture on radiographs: discriminant value in postmenopausal osteoporosis. Osteoporos Int. 1998;8:618–25.

    Article  PubMed  Google Scholar 

  11. Ikuta A, Kumasaka S, Kashima I. Quantitative analysis using the star volume method applied to skeleton patterns extracted with a morphological filter. J Bone Miner Metab. 2000;18:271–7.

    Article  PubMed  Google Scholar 

  12. Vesterby A, Gunndersen HJG, Melsen F. Star volume of marrow space and trabeculae of the first lumbar vertebra: sampling efficiency and biological variation. Bone. 1989;10:7–13.

    Article  PubMed  Google Scholar 

  13. Garrahan NJ, Mellich RW, Compston JE. A new method for the two-dimensional analysis of bone structure in human iliac crest biopsies. J Microsc. 1989;142:341–9.

    Article  Google Scholar 

  14. Fuller R. Probiotics in man and animals. J Appl Bacteriol. 1989;66:365–78.

    Article  PubMed  Google Scholar 

  15. Joint FAO/WHO expert consultation. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Argentina: World Health Organization; 2001.

    Google Scholar 

  16. Spanhaak S, Havenaar R, Schaafsma G. The effect of consumption of milk fermented by Lactobacillus cassei strain shirota on the intestinal microflora and immune parameters in humans. Eur J Clin Nutr. 1998;52:899–907.

    Article  PubMed  Google Scholar 

  17. Yamano T, Iino H, Takada M, Blum S, Rochat F, Fukushima Y. Improvement of human intestinal flora by ingestion of a probiotic strain of Lactobacillus johnsonii La1. Br J Nutr. 2006;95:303–12.

    Article  PubMed  Google Scholar 

  18. Ishida Y, Nakamura F, Kanzato H, Sawada D, Hirata H, Nishimura A, et al. Clinical effects of Lactobacillus acidophilus strain L-92 on perennial allergic rhinitis: a double-blind, placebo controlled study. J Dairy Sci. 2005;88:527–33.

    Article  PubMed  Google Scholar 

  19. Fujiwara D, Wakabayashi H, Watanabe H, Nishida S, Iino H. A double-blind trial of Lactobacillus paracasei strain KW3110 administration for immunomodulation in patients with pollen allergy. Allergol Int. 2005;54:143–9.

    Article  Google Scholar 

  20. Kalliomaki M, Salminen S, Arvilommi H, Kero P, Koskinen P, Isolauri E. Probiotics in primary prevention of atopic disease: a randomized placebo-controlled trial. Lancet. 2001;357:1076–9.

    Article  PubMed  Google Scholar 

  21. Isolauri E, Arvola T, Sütas Y, Moilanen E, Salminen S. Probiotics in the management of atopic eczema. Clin Exp Allerg. 2000;30:1605–10.

    Article  Google Scholar 

  22. Aso Y, Akaza H, Kotake T, Tsukamoto T, Imai K, Naito S. Preventive effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer in double-blind trial. The BLP Study Group. Eur Urol. 1995;27:104–9.

    PubMed  Google Scholar 

  23. Nagao F, Nakayama M, Muto T, Okumura K. Effects of a fermented milk drink containing Lactobacillus casei strain shirota on the immune system in healthy human subjects. Biosci Biotechnol Biochem. 2000;64:2706–8.

    Article  PubMed  Google Scholar 

  24. Link-Amster H, Rochat F, Saudan KY, Mignot O, Aeschlimann JM. Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk intakes. FEMS Immunol Med Microbiol. 1994;10:55–64.

    Article  PubMed  Google Scholar 

  25. Schiffrin EJ, Rochat F, Link-Amster H, Aeschlimann JM, Donnet-Hughes A. Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. J Dairy Sci. 1995;78:491–7.

    Article  PubMed  Google Scholar 

  26. Weizman Z, Asli G, Alsheikh A. Effect of a probiotic infant formula on infections in child care centers: comparison of two probiotic agents. Pediatrics. 2005;115:5–9.

    PubMed  Google Scholar 

  27. Hatakka K, Savilahti E, Ponka A, Meurman JH, Poussa T, Nase L, et al. Effect of long term consumption of probiotic milk on infections in children attending day care centres: double blind, randomized trial. BMJ. 2001;322:15.

    Article  Google Scholar 

  28. Nase L, Hatakka K, Sacilahti E, Saxelin M, Ponka A, Poussa T, et al. Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res. 2001;35:412–20.

    Article  PubMed  Google Scholar 

  29. Kimoto-Nira H, Suzuki C, Kobayashi M, Sasaki K, Kurisaki J, Mizumachi K. Anti-ageing effect of a lactococcal strain: analysis using senescence-accelerated mice. Br J Nutr. 2007;98:1178–86.

    Article  PubMed  Google Scholar 

  30. Hinoi E, Takarada T, Uno K, Inoue M, Murafuji Y, Yoneda Y. Glutamate suppresses osteoclastogenesis through the cystine/glutamate antiporter. Am J Pathol. 2007;170:1277–90.

    Article  PubMed  Google Scholar 

  31. Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. 2003;423:337–42.

    Article  PubMed  Google Scholar 

  32. Harada S, Rodan GA. Control of osteoblast function and regulation of bone mass. Nature. 2003;423:349–55.

    Article  PubMed  Google Scholar 

  33. Chenu C, Serre CM, Raynal C, Burt-Pichat B, Delmas PD. Glutamate receptors are expressed by bone cells and are involved in bone resorption. Bone. 1998;22:295–9.

    Article  PubMed  Google Scholar 

  34. Mentaverri R, Kamel S, Wattel A, Prouillet C, Sevenet N, Petit JP, et al. Regulation of bone resorption and osteoclast survival by nitric oxide: possible involvement of NMDA-receptor. J Cell Biochem. 2003;88:1145–56.

    Article  PubMed  Google Scholar 

  35. Peet NM, Grabowski PS, Laketic-Ljubojevic I, Skerry TM. The glutamate receptor antagonist MK801 modulates bone resorption in vitro by a mechanism predominantly involving osteoclast differentiation. FASEB J. 1999;13:2179–85.

    PubMed  Google Scholar 

  36. Morimoto R, Uehara S, Yatsushiro S, Juge N, Hua Z, Senoh S, et al. Secretion of L-glutamate from osteoclasts through transcytosis. EMBO J. 2006;25:4175–86.

    Article  PubMed  Google Scholar 

  37. Hinoi E, Fujimori S, Yoneda Y. Modulation of cellular differentiation by N-methyl-D-aspartate receptors in osteoblast. FASEB J. 2003;17:1532–4.

    PubMed  Google Scholar 

  38. Matsubara M, Yamachika E, Tsujigiwa H, Mizukawa N, Ueno T, Murakami J, et al. Suppressive effects of 1,4-dihydroxy-2-naphthoic acid administration on bone resorption. Osteoporos Int. 2010;21:1437–47.

    Article  PubMed  Google Scholar 

  39. Tsugawa N, Shiraki M, Suhara T, Kamao M, Tanaka K, Okano T. Vitamin K status of healthy Japanese women: age-related vitamin K requirement for γ-carboxylation of osteocalcin. Am Clin Nutr. 2006;83:380–6.

    Google Scholar 

  40. Miyake M, Kozai Y, Sakurai T, Kashima I. Effects of citric acid administration on femoral trabecular structures in ovariectomized mice. Oral Radiol. 2007;23:34–41.

    Article  Google Scholar 

  41. Takano T, Kozai Y, Kawamata R, Wakao H, Sakurai T, Kashima I. Inhibitory effect of maritime pine bark extract (Pycnogenol®) on deterioration of bone structure in the distal femoral epiphysis of ovariectomized mice. Oral Radiol. 2011;27:8–16.

    Article  Google Scholar 

  42. Ushiyama S, Yamaguchi M. Inhibitory effects of β-cryptoxanthin on osteoclast-like cell formation in mouse marrow cultures. Biochem Pharmacol. 2004;67:1297–305.

    Article  Google Scholar 

Download references

Conflict of interest

There are no financial or other relationships that could lead to a conflict of interest.

Author information

Authors and Affiliations

  1. Division of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College, 82 Inaoka, Yokosuka, Kanagawa, 238-8580, Japan

    Toshinaga Miura, Yusuke Kozai, Ryota Kawamata, Hiromi Wakao, Takashi Sakurai & Isamu Kashima

Authors
  1. Toshinaga Miura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yusuke Kozai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ryota Kawamata
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiromi Wakao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takashi Sakurai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Isamu Kashima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yusuke Kozai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Miura, T., Kozai, Y., Kawamata, R. et al. Inhibitory effect of a fermented soy product from lactic acid bacteria (PS-B1) on deterioration of bone mass and quality in ovariectomized mice. Oral Radiol 30, 45–52 (2014). https://doi.org/10.1007/s11282-013-0143-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11282-013-0143-3

Keywords

Search

Navigation