ORIGINAL ARTICLE

Oral Radiology

, Volume 23, Issue 2, pp 34-41

Effects of citric acid administration on femoral trabecular structures in ovariectomized mice

  • Masayasu MiyakeAffiliated withDivision of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College
  • , Yusuke KozaiAffiliated withDivision of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College
  • , Takashi SakuraiAffiliated withDivision of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College Email author 
  • , Isamu KashimaAffiliated withDivision of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College

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Abstract

Objectives

To examine the effects of citric acid administration on trabecular structures.

Methods

Ovariectomized female ICR mice (n = 39), 8 weeks of age, were divided into four groups: ovariectomized control (OVX-control, n = 9, standard diet); citric acid (CIT, n = 10, citric acid 5 g/100 g diet); vitamin K2 (menaquinone 7; VK2, n = 10, vitamin K2 50 µg/100 g diet); and citric acid + vitamin K2 (CIT + VK2, n = 10, citric acid 5 g/100 g diet and vitamin K2 50 µg/100 g diet). After 12 weeks, the bone mass of the right femur was measured using peripheral quantitative computed tomography and the three-dimensional (3-D) trabecular structure of the right femur was assessed using microfocus computed tomography.

Results

The bone mineral density was significantly increased in the VK2 group versus the OVX-control group (P < 0.05). In the 3-D trabecular structure analysis, skeletal perimeter and number were significantly greater in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group. Skeletal separation, spacing, and trabecular bone pattern factor were significantly lower in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group. Almost all parameters in node–strut analysis were significantly greater in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group.

Conclusions

These results suggest that citric acid improved trabecular structures. However, simultaneous administration of citric acid and vitamin K2 did not have any additional effect.

Key words

Osteoporosis Mice Citric acid Vitamin K2