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Screening of immunomodulatory and adhesive Lactobacillus with antagonistic activities against Salmonella from fermented vegetables

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Abstract

The purpose of this study was to select strains of lactic acid bacteria (LAB) by their in vitro adhesive and immunomodulatory properties for potential use as probiotics. In this study, 16 randomly selected LAB strains from fermented vegetables (sauerkraut, bean and cabbage) were first screened for their tolerance to acid, bile salts, pepsin and pancreatin, bacterial inhibitory activities and abilities to adherence to Caco-2 cells. Then, 4 strains with the highest adhesion abilities were selected for further studies of their immunomodulatory properties and inhibitory effects against Salmonella adhesion and invasion to Caco-2 cells in vitro. The results showed that these 16 LAB strains effectively survived in simulated gastrointestinal condition and inhibited growth of six tested pathogens. Lactobacillus rhamnosus P1, Lactobacillus plantarum P2, Lactobacillus rhamnosus P3 and Lactobacillus casei P4 had the highest abilities to adhere to Caco-2 cells. Furthermore, L. plantarum P2 strain showed higher abilities to induce expression of tumor necrosis factor-α and interleukin-12 by splenic monocytes and strongly inhibited the adhesion and invasion of S. enteritidis ATCC13076 to Caco-2 cells. These results suggest that Lactobacillus strains P2 could be used as a probiotic candidate in food against Salmonella infection.

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Acknowledgments

This work was supported by funding from a innovation project of science and technology plan project of Shaanxi Province, China (2014KTCL02-21) and a Ministry of Agriculture (No. 2013-S16), and the thousand talent program to X. Z. and Natural Science Foundation of China (No. 31402095) and Northwest A&F University PhD Research Start-up funds (No. 2012BSJJ094) to X. Y.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Research Building 4208, Yangling, 712100, Shaanxi, People’s Republic of China

    Junchang Feng, Pilong Liu, Xin Yang & Xin Zhao

  2. Department of Animal Science, McGill University, Ste. Anne de Bellevue, Quebec, Canada

    Xin Zhao

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  1. Junchang Feng
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  2. Pilong Liu
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  3. Xin Yang
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  4. Xin Zhao
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Correspondence to Xin Zhao.

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Feng, J., Liu, P., Yang, X. et al. Screening of immunomodulatory and adhesive Lactobacillus with antagonistic activities against Salmonella from fermented vegetables. World J Microbiol Biotechnol 31, 1947–1954 (2015). https://doi.org/10.1007/s11274-015-1939-6

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  • DOI: https://doi.org/10.1007/s11274-015-1939-6

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