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Chitinolytic Streptomyces vinaceusdrappus S5MW2 isolated from Chilika lake, India enhances plant growth and biocontrol efficacy through chitin supplementation against Rhizoctonia solani

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Abstract

A chitinolytic actinomycete Streptomyces vinaceusdrappus S5MW2 was isolated from water sample of Chilika lake, India and identified using 16S rRNA gene sequencing. It showed in vitro antifungal activity against the sclerotia producing pathogen Rhizoctonia solani in a dual culture assay and by chitinase enzyme production in a chitin supplemented minimal broth. Moreover, isolate S5MW2 was further characterized for biocontrol (BC) and plant growth promoting features in a greenhouse experiment with or without colloidal chitin (CC). Results of greenhouse experiment showed that CC supplementation with S5MW2 showed a significant growth of tomato plants and superior disease reduction as compared to untreated control and without CC treated plants. Moreover, higher accumulation of chitinase also recovered in the CC supplemented plants. Significant effect of CC also concurred with the Analysis of Variance of greenhouse parameters. These results show that the a marine antagonist S5MW2 has BC efficiency against R. solani and chitinase enzyme played important role in plant resistance.

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References

  • Anitha A, Rabeeth M (2009) Control of Fusarium wilt of tomato by bioformulation of Streptomyces griseus in greenhouse condition. Afr J Basic Appl Sci 1:9–14

    Google Scholar 

  • Boller T, Mauch F (1988) Colourimetric assay for chitinase. Method Enzymol 161:430–435

    Article  CAS  Google Scholar 

  • Boudjella H, Baute K, Zitoune A, Mathieu F, Lebsehi A, Sabaou N (2006) Taxonomy and chemical characterization of antibiotics of Streptosporangium sg 10 isolated from a Saharan soil. Microbiol Res 161:288–298

    Article  CAS  Google Scholar 

  • El-Dein A, Hosny MS, El-Shayeb NA, Abood A, Abdel-Fattah AM (2010) A potent chitinolytic activity of marine actinomycete sp. and enzymatic production of chitooligosaccharides. Aust J Basic Appl Sci 4:615–623

    Google Scholar 

  • El-Tarabily KA, Sivasithamparam K (2006) Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biol Biochem 38:1505–1520

    Article  CAS  Google Scholar 

  • Glass C, Silverstein JA, Denton L (1997) Bacterial populations in activated sludge denitrifying high nitrate waste reflect pH differences. In: Proceedings, second international conference on microorganisms in activated sludge and biofilm processes, Berkely, pp 377–380

  • Gopalakrishnan S, Pande S, Sharma M, Humayun P, Kiran BK, Sandeep D, Vidya MS, Deepthi K, Rupela O (2011) Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea. Crop Prot 30:1070–1078

    Article  CAS  Google Scholar 

  • Hoster F, Schmitz JE, Daniel R (2005) Enrichment of chitinolytic microorganisms: isolation and characterization of a chitinase exhibiting antifungal activity against phytopathogenic fungi from a novel Streptomyces strain. Appl Microbiol Biotechnol 66:434–442

    Article  CAS  Google Scholar 

  • Huang X, Madan A (1999) CAP3: a DNA sequence assembly program. Genome Res 9:868–877

    Article  CAS  Google Scholar 

  • Jeuniaux C, Voss-Foucart MF (1991) Chitin biomass and production in the marine environment. Biochem Syst Ecol 19:347–356

    Article  CAS  Google Scholar 

  • Khamna S, Yokota A, Lumyong S (2009) Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World J Microbiol Biotechnol 25:649–655

    Article  CAS  Google Scholar 

  • Kishore GK, Pande S, Podile AR (2005) Chitin-supplemented foliar application of Serratia marcescens GPS 5 improves control of late leaf spot disease of groundnut by activating defense-related enzymes. J Phytopathol 153:169–173

    Article  CAS  Google Scholar 

  • Malviya N, Yadav AK, Yandigeri MS, Arora DK (2011) Diversity of culturable Streptomycetes from wheat cropping system of fertile regions of Indo-Gangetic Plains, India. World J Microbiol Biotechnol 27:1593–1602

    Article  CAS  Google Scholar 

  • Maruyama A, Maeda M, Simidu U (1989) Microbial production of auxin indole-3-acetic acid in marine sediments. Mar Ecol Prog Ser 58:69–75

    Article  CAS  Google Scholar 

  • Pandey B, Ghimire P, Agrawal VP (2004) Studies on antibacterial activity of soil from Khumbu region of mount everest In: International conference on the great himalayas: climate, health, ecology, management and conservation, Kathmandu University, Nepal and the Aquatic Ecosystem Health and Management Society, Canada, p 53

  • Patil HJ, Srivastava AK, Singh DP, Chaudhari BL, Arora DK (2011) Actinomycetes mediated biochemical responses in tomato (Solanum lycopersicum) enhances bioprotection against Rhizoctonia solani. Crop Prot 30:1269–1273

    Article  CAS  Google Scholar 

  • Patten C, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42:207–220

    Article  CAS  Google Scholar 

  • Pisano MA, Sommer MJ, Taras L (1992) Bioactivity of chitinolytic actinomycetes of marine origin. Appl Microbiol Biotechnol 36:553–555

    Article  CAS  Google Scholar 

  • Ramesh S, Mathivanan N (2009) Screening of marine actinomycetes isolated from the Bay of Bengal, India for antimicrobial activity and industrial enzymes. World J Microbiol Biotechnol 25:2103–2111

    Article  CAS  Google Scholar 

  • Robbins PW, Albright C, Benfield B (1998) Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli. J Biol Chem 263:443–447

    Google Scholar 

  • Schippers B, Bakker AW, Bakker PAHM, Peer R (1990) Beneficialand deleterious effects of HCN-producing pseudomonads on rhizosphere interactions. Plant Soil 129:75–83

    Article  CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Solanki MK, Singh N, Singh RK, Singh P, Srivastava AK, Kumar S, Kashyap PL, Arora DK (2011) Plant defense activation and management of tomato root rot by a chitin-fortified Trichoderma/Hypocrea formulation. Phytoparasitica 39:471–481

    Article  CAS  Google Scholar 

  • Solanki MK, Kumar S, Panday AK, Srivastava S, Singh RK, Kashyap PL, Srivastava AK, Arora DK (2012) Diversity and antagonistic potential of Bacillus spp. associated to the rhizosphere of tomato for the management of Rhizoctonia solani. Biocontrol Sci Technol 22:203–217

    Article  Google Scholar 

  • Szczech M, Shoda M (2006) The effect of mode of application of Bacillus subtilis RB14C on its efficacy as a biocontrol agent against Rhizoctonia solani. J Phytopathol 154:370–377

    Article  CAS  Google Scholar 

  • Tripathi BM, Kaushik R, Kumari P, Saxena AK, Arora DK (2011) Genetic and metabolic diversity of streptomycetes in pulp and paper mill effluent treated crop fields. World J Microbiol Biotechnol 27:1603–1613

    Article  Google Scholar 

  • Tsavkelova EA, Klimova SY, Cherdyntseva TA, Netrusov AI (2006) Microbial producers of plant growth stimulators and their practical use: a review. Appl Biochem Microbiol 42:117–126

    Article  CAS  Google Scholar 

  • Vijayakumar R, Gopika G, Dhanasekaran D, Saravanamuthu R (2012) Isolation, characterisation and antifungal activity of marine actinobacteria from Goa and Kerala, the west coast of India. Arch Phytopathol Plant Prot 45:1010–1025

    Article  CAS  Google Scholar 

  • Yandigeri MS, Meena KK, Singh D, Malviya N, Singh DP, Solanki MK, Yadav AK, Arora DK (2012) Drought-tolerant endophytic actinobacteria promote growth of wheat (Triticum aestivum) under water stress conditions. Plant Growth Regul 68:411–420

    Article  CAS  Google Scholar 

  • You JL, Cao LX, Liu GF, Zhou SN, Tan HM, Lin YC (2005) Isolation and characterization of actinomycetes antagonistic to pathogenic Vibrio spp. from nearshore marine sediments. World J Microbiol Biotechnol 21:679–682

    Article  Google Scholar 

  • Yu T, Wang L, Yin Y, Wang Y, Zheng X (2008) Effect of chitin on the antagonistic activity of Cryptococcus aurentii against Penicillium expansum in pear fruit. Int J Food Microbiol 122:44–48

    Article  CAS  Google Scholar 

  • Yuan WM, Crawford DL (1995) Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl Environ Microbiol 61:3119–3128

    CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by the Indian Council of Agriculture Research (ICAR) by a network project ‘Application of Microorganisms in Agriculture and Allied Sectors (AMAAS)’. The help of the culture collection unit of NBAIM is highly appreciated for providing cultures for this study. All the co-authors are acknowledged for help in execution of experiments, data collection, analysis and manuscript preparation.

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Correspondence to Mahesh S. Yandigeri.

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Yandigeri, M.S., Malviya, N., Solanki, M.K. et al. Chitinolytic Streptomyces vinaceusdrappus S5MW2 isolated from Chilika lake, India enhances plant growth and biocontrol efficacy through chitin supplementation against Rhizoctonia solani . World J Microbiol Biotechnol 31, 1217–1225 (2015). https://doi.org/10.1007/s11274-015-1870-x

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