Abstract
In the present study bacterial strains were isolated from the rumen fluids of Bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens Vibrio cholera, Enterococcus faecalis, Enterobacter aerogens, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. Moreover it showed above 70 % cell surface hydrophobicity, significant low-invasion ability and potential adherence capacity in Caco-2 cells when compared with the control. The proinflammatory cytokines (TNF-α) was greatly reduced in rumen bacteria treatment and ARBS-1 modulate the immune response by activating the IL-4 secretion in parallel to TNF-α suppression. The 16s rRNA gene sequence of the active isolates were identified as Enterococcus hirae (ARBS-1), Pediococcus acidilactici (ARBS-4) and Bacillus licheniformis (ARBS-7). This study revealed the probiotic bactericidal properties of E. hirae obtained from the rumen of B. primigenius with potential antibacterial and anti-inflammatory effects. Future studies with the strains may yield some novel probiotic product for livestock’s.
This is a preview of subscription content, log in via an institution to check access.
References
Alcaine SD, Warnick LD, Wiedmann M (2007) Antimicrobial resistance in non typhoidal salmonella. J Food Prot 70:780–790
Asahara T, Shimizu K, Nomoto K, Hamabata T, Ozawa A, Takeda Y (2004) Probiotic bifidobacteria protect mice from lethal infection with Shiga toxin-producing Escherichia coli O157: H7. Infect Immun 72:2240–2247
Doron SI, Hibberd PL, Gorbach SL (2008) Probiotics for prevention of antibiotic-associated diarrhea. J Clin Gastroenterol 42:58–63
Espeche MC, Pellegrino M, Frola I, Larriestra A, Bogni C, Nader-Macias ME (2012) Lactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis. Anaerobe 18:103–109
Fitch WM, Margoliash E (1967) Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284
Gill HS, Cross ML, Rutherfurd KJ (2001a) Dietary probiotic supplementation to enhance cellular immunity in the elderly. Br J Biomed Sci 58:94–96
Gill HS, Rutherfurd KJ, Cross ML (2001b) Dietary probiotic supplementation enhances natural killer cell activity in the elderly: an investigation of age-related immunological changes. J Clin Immunol 4:264–271
Hairul Islam VI, Prakash Babu N, Pandikumar P, Ignacimuthu S (2011) Isolation and characterization of putative probiotic bacterial strain, Bacillus amyloliquefaciens, from North East Himalayan soil based on in vitro and in vivo functional properties. Probiotics Antimicrob Prot 3:175–185
Hairul Islam VI, Preetam Raj JP, Gabriel Paulraj M, Ignacimuthu S (2013) Myroides pelagicus from the gut of Drosophila melanogaster attenuates inflammation on dextran sodium sulfate-induced colitis. doi:10.1007/s10620-013-3010-5
Khampa S, Wanapat M, Wachirapakorn C, Nontaso N, Wattiaux M (2006) Effects of urea level and sodium DL-malate in concentrate containing high cassava chip on ruminal fermentation efficiency, microbial protein synthesis in lactating dairy cows raised under tropical condition. Asian Australas J Anim Sci 19:837–844
Kiely LJ, Olson NF (2000) The physicochemical surface characteristics of Lactobacillus casei. Food Microbiol 17:277–291
Kravtsov EG, Yermolayev AV, Anokhina IV, Yashina NV, Chesnokova VL, Dalin MV (2008) Adhesion characteristics of lactobacillus is a criterion of the probiotic choice. Bull Exp Biol Med 145:232–234
Mamen D, Vadivel V, Pugalenthi M, Parimelazhagan T (2010) Evaluation of fibrolytic activity of two different anaerobic rumen fungal isolates for their utilization as microbial feed additive. Animal Nutr Feed Technol 10:37–49
Matsumoto S, Hara T, Hori T, Mitsuyama K, Nagaoka M, Tomiyasu N, Suzuki A, Sata M (2005) Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down- regulation of pro-inflammatory cytokines in lamina propria mononuclear cells. Clin Exp Immunol 140:417–426
Mera AM, Aparicio LR, Rua J, Blanco HM, Navasa N, Armesto MRG, Ferrero MA (2012) In vitro evaluation of physiological probiotic properties of different lactic acid bacteria strains of dairy and human origin. J Funct Foods 4:531–541
Mikulski D, Jankowski J, Naczmanski J, Mikulska M, Demey V (2012) Effects of dietary probiotic [Pediococcus acidilactici] supplementation on performance, nutrient digestibility, egg traits, egg yolk cholesterol, and fatty acid profile in laying hens. Poult Sci 91:2691–2700
Millette M, Luquet FM, Lacroix M (2007) In vitro growth control of selected pathogens by Lactobacillus acidophilus and Lactobacillus casei-fermented milk. Lett Appl Microbiol 44:314–319
Moorthy G, Murali MR, Devaraj SN (2007) Protective role of lactobacilli in Shigella dysenteriae 1-induced diarrhea in rats. Nutrition 23:424–433
Nostro A, Cannatelli MA, Crisafi G, Musolino AD, Procopio F, Alonza V (2004) Modification of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helychryssum italicum extract. Lett Appl Microbiol 38:423–427
Perdigón G, Maldonado GC, Valdez JC, Medici M (2002) Inter-action of lactic acid bacteria with the gut immune system. Eur J Clin Nutr 56:21–26
Pereira DI, McCartney AL, Gibson GR (2003) An in vitro study of the probiotic potential of a bile salt hydrolyzing Lactobacillus fermentum strain, and determination of its cholesterol lowering properties. Appl Environ Microbiol 69:4743–4752
Ram C, Chander H (2003) Optimization of culture conditions of probiotic bifidobacteria for maximal adhesion to hexadecane. World J Microbiol Biotechnol 19:407–410
Reid G (2006) Probiotics to prevent the need for, and augment the use of antibiotics. Can J Infect Dis Med Microbiol 17:291–295
Roos S, Jonsson H (2002) A high-molecular-mass cell-surface protein from Lactobacillus reuteri 1063 adheres to mucus components. Microbiology 148:433–442
Sarinena S, Barlow J, Costabile A, Gibson GR, Rowland I (2012) In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids. Anaerobe 18:530–538
Succi M, Tremonte P, Reale A, Sorrentino E, Grazia L, Pacifico S, Coppola R (2005) Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese. FEMS Microbiol 244:129–137
Vidhyasagar V, Jeevaratnam K (2013) Evaluation of Pediococcus pentosaceus strains isolated from idly batter for probiotic properties in vitro. J Funct Foods 5:235–243
Yang ZG, Sun HX, Fang WH (2005) Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice. Vaccine 23:5196–5203
Acknowledgments
The authors would like to thank the Seoul National University and National Institute of Animal Science (NIAS), Republic of Korea, for its unstinted support and Pondicherry Centre for Biological Science, Pondicherry, India for carrying out the antimicrobial assay.
Conflict of interest
The authors declare that there are no conflicts of interest in the work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Arokiyaraj, S., Hairul Islam, V.I., Bharanidharan, R. et al. Antibacterial, anti-inflammatory and probiotic potential of Enterococcus hirae isolated from the rumen of Bos primigenius . World J Microbiol Biotechnol 30, 2111–2118 (2014). https://doi.org/10.1007/s11274-014-1625-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-014-1625-0