Abstract
A new yeast species (KKU-FW10) belonging to the Candida genus was isolated from Jasminum adenophyllum in the Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn area, Chulabhorn Dam, Konsan district within Chaiyaphum province in Thailand. The strain was identified via analysis of nucleotide sequences from the D1/D2 domain of 26S ribosomal DNA and based on its morphological, physiological and biochemical characteristics. The sequence obtained from yeast isolate KKU-FW10 was 97 percent identical to that of Candida chanthaburiensis (GenBank accession number AB500861.1), with 506/517 (nucleotides identity/total nucleotides) matching nucleotides, nine substitutions and two gaps being detected. This species belonged to the Candida clade. Regarding morphological characteristics, isolate KKU-FW10 presents cream-colored butyrous colonies, vegetative reproduction through budding and, round cells without filaments or ascospores. The major ubiquinone detected was Q-9. The above results suggest that isolate KKU-FW10 is a new member of the genus Candida, and the name Candida konsanensis is proposed for this yeast. The type strain of the new species is KKU-FW10T (= BCC 52588T, = NBRC 109082T, = CBS 12666T). In addition, this KKU-FW10 could potentially produce 58.24 Units/ml of carboxymethyl cellulase when it was cultured in YP broth containing 1.0 % carboxymethyl cellulose for 24 h.
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References
Boonmark C, Jindamorakot S, Kawasaki H, Yongmanitchai W, Suwanarit P, Nakase T, Limtong S (2009) Candida siamensis sp. Nov., an anamorphic yeast in the Saturnispora clade isolated in Thailand. FEMS Yeast Res 9:668–672
Ekperigin MM (2007) Preliminary studies of cellulase production by Acinetobacter anitratus and Branhamella sp. Afr J Biotechnol 6:28–33
Farkas V, Viskova M, Biely P (1985) Novel media for detection of microbial producers of cellulase and xylanase. FEMS Microbial Lett 28:137–140
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Jindamorakot S, Ninomiya S, Limtong A, Yongmanitchai W, Tuntirungkij M, Potachaoen W, Tanaka K, Kawasaki H, Nakase T (2009) Three new species of bipolar budding yeasts of genus Hanseniaspora and its anamorph Kloeckera isolated in Thailand. FEMS Yeast Res 9:1327–1337
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek J Microbiol 73:331–371
Lachance M, Bowles JM, Starmer WT, Barker SF (1999) Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45:172–177
Lachance M, Bowles JM, Starmer WT (2003) Metschnikowia santaceciliae, Candida hawaiiana, and Candida kipukae, three new yeast species associated with insects of tropical morning glory. FEMS Yeast Res 3:97–103
Limtong S, Yongmanitchai W (2010) Candida chanthaburiensis sp. nov., Candida kungkrabaensis sp. nov. and Candida suratensis sp. nov., three novel yeast species from decaying plant materials submerged in water of mangrove forests. Antonie Van Leeuwenhoek J Microbiol 98:379–388
Limtong S, Yongmanitchai W, Kawasaki H, Seki T (2007) Candida thaimueangensis sp. nov., an anamorphic yeast species from estuarine water in a mangrove forest in Thailand. Int J Syst Evol Microbiol 57:650–653
Limtong S, Imanishi Y, Jindamorakot S, Ninomiya S, Yongmanitchai W, Nakase T (2008) Torulaspora maleeae sp. Nov., a novel ascomycetous yeast species from Japan and Thailand. FEMS Yeast Res 8:337–343
Limtong S, Jindamorakot S, Am-in S, Kaewwichian R, Nitiyon S, Yongmanitchai W, Nakase T (2011) Candida uthaithanina sp. nov., a anamorphic yeast species in Nakaseomyces clade isolated in Thailand. Antonie van Leeuwenhoek J Microbiol 99:865–871
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Nakase T, Suzuki M (1986) The ubiquinone system in strains of species in the ballistospore-forming yeast genera Sporidiobolus, Sporobolomyces and Bullera. J Gen Appl Microbiol 32:251–258
Nakase T, Lee FL, Am-in S, Jindamorakot S, Potachaoen W, Tanticharoen M, Takashima M (2005) Sporobolomyces fushanensis sp. nov., a new species of ballistoconidium-forming yeast in the microbotryum lineage isolated from a plant in Taiwan. J Gen Appl Microbiol 51:41–45
Phaff HJ, Starmer WT, Lachance MA, Ganter PF (1994) Candida caseinolytica sp. nov., a new species of yeast occurring in necrotic tissue of Opunta and Stenocereus species in the Southwestern United States and Baja California. Mexico. Int J Syst Evol Microbiol 44:641–645
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sengupta S, Jana ML, Sengupta D, Naskar AK (2000) A note on the estimation of microbial glycosidase activities by dinitrosalicylic acid reagent. Appl Microbiol Biotechnol 53:732–735
Tavanti A, Davidson AD, Gow NAR, Maiden MCJ, Odds FC (2005) Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol 43:284–292
Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777–780
Wang Q, Bai F (2004) Four new yeast species of the genus Sporobolomyces from plant leaves. FEMS Yeast Res 4:579–586
Wood TM, McCrae SI (1986) The cellulase of Penicillium pinophilum: synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases. Biochem J 234:93–99
Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts: a taxonomic study. Elsevier Science Publishers, Amsterdam, pp 77–100
Acknowledgments
The authors thank the Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn: Khon Kaen University for permission to conduct the research and the Electricity Generating Authority of Thailand (EGAT) for field support. This work was partially supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through the Biofuel Cluster of Khon Kaen University. We thank to KKU Publication Clinic for providing professional editing service from American Journal Experts.
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Sarawan, S., Mahakhan, P., Jindamorakot, S. et al. Candida konsanensis sp. nov., a new yeast species isolated from Jasminum adenophyllum in Thailand with potentially carboxymethyl cellulase-producing capability. World J Microbiol Biotechnol 29, 1481–1486 (2013). https://doi.org/10.1007/s11274-013-1313-5
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DOI: https://doi.org/10.1007/s11274-013-1313-5