Abstract
Three hyper 2-propanol producing strains were isolated from Singapore environment using an enrichment step and a high through-put screening step. The analysis of the amplified 16S rDNA revealed that the isolates belonged to Clostridium species and they were named as Clostridium sp. BT10-1, Clostridium sp. M10-1 and Clostridium sp. PU31-4. At 1 L scale, the 2-propanol titer of these positive strains was 1.6–2.1 times of that of Clostridium beijerinckii NRRL B593, which is so far the most efficient natural 2-propanol producer. The highest 2-propanol titer was achieved by isolate BT10-6 and it was 5.26 g/L (87.5 mM). These three positive strains BT10-6, M10-1 and PU31-4 consumed glucose almost completely in 40–48 h and gave 2-propanol productivity at 0.132, 0.118 and 0.087 g/L/h, respectively, which is 3.0–4.6 times of 0.029 g/L/h given by C. beijerinckii NRRL B593. Butanol was also produced by these positive strains with a slightly lower butanol titer and higher butanol productivity, compared to butanol control strain C. beijerinckii NCIMB 8052.
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This work was supported by the Agency for Science, Technology and Research (A*star), Singapore (ICES/08-374B01) and was collaborated with Mitsui Chemical Singapore R&D Centre, Pte Ltd.
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Ng, Z.R., Takahashi, K. & Liu, Z. Isolation, characterization and evaluation of hyper 2-propanol producing bacteria from Singapore environment. World J Microbiol Biotechnol 29, 1059–1065 (2013). https://doi.org/10.1007/s11274-013-1269-5
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DOI: https://doi.org/10.1007/s11274-013-1269-5