Abstract
The endophytic fungus XJ-AC03, which was isolated from the healthy roots of Aconitum leucostomum, produced aconitine when grown in potato dextrose agar (PDA) medium. The presence of aconitine was confirmed by the chromatographic and spectroscopic analyses. The yield of aconitine was recorded as 236.4 μg/g by high performance liquid chromatography (HPLC). The mass spectrometry was shown to be identical to authentic aconitine. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal aconitine indicated that the fungal aconitine produced an NMR spectrum identical to that of authentic aconitine. Strain XJ-AC03 was identified as Cladosporium cladosporioides by its characteristic culture morphology and ITS rDNA sequence analysis.
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Acknowledgments
This work was supported by Scientific and Technological Project in Shaanxi Province (Grant No. 14210114). We thank Shuang Xia for his assistance in collection of A. leucostomum samples, Prof. Li for helping with identification of the plant material. We are grateful to other colleagues in the College of Animal Veterinary Medicine, Northwest A&F University. We also wish to thank the anonymous reviewers for constructive comments.
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Yang, K., Liang, J., Li, Q. et al. Cladosporium cladosporioides XJ-AC03, an aconitine-producing endophytic fungus isolated from Aconitum leucostomum . World J Microbiol Biotechnol 29, 933–938 (2013). https://doi.org/10.1007/s11274-012-1246-4
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DOI: https://doi.org/10.1007/s11274-012-1246-4