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Optimization and/or acclimatization of activated sludge process under heavy metals stress

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Abstract

The present study aimed to overcome the toxicity of the heavy metals load, discharged with the industrial effluents into Alexandria sewerage network, on the activated sludge treatment system through effective acclimation for organic matter and heavy metals removal. Optimization and/or acclimatization of the activated sludge process in the presence of Cu, Cd, Co and Cr contaminating mixed domestic-industrial wastewater was investigated. Acclimatization process was performed through abrupt and stepwise addition of tested metals using sequencing batch reactors treatment approach and evaluated as microbial oxygen uptake rate (OUR), dehydrogenase activity (DHA), organic matter (COD) and heavy metals removal. Abrupt addition of metals adversely affected sludge bioactivity leading to decline in the removal efficiency of the targeted contaminants and loss of floc structure. Metals IC50 confirmed that copper possessed the highest toxicity towards the OUR, DHA activity and COD removal with orders Cu > Cd > Cr > Co; Cu > Cd > Co = Cr and Cu > Cd > Cr > Co, respectively. The highest metal removal was recorded for Cd followed by Co, Cu and finally Cr, most of which was retained in the dissolved influent. However, controlled stepwise application of the tested metals exhibited high sensitivity of DHA and OUR activities only at the highest metal concentrations although enhanced at the lowest concentrations while COD removal was not significantly affected. In conclusion, this approach resulted in adaptation of the system where sludge microbes acquired and developed natural resistance to such metals leading to remarkable enhancement of both organic matter and heavy metals removal.

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The authors declare that they have no conflict of interest.

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Author notes

  1. Ebtesam El Bestawy

    Present address: Department of Life Sciences, Faculty of Science, King Abdul Aziz University, P.O. Box 42805, Jeddah, 21551, Kingdom of Saudi Arabia

Authors and Affiliations

  1. Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, 163 Horria Ave. El-Shatby, P.O. Box 832, Alexandria, Egypt

    Ebtesam El Bestawy & Shacker Helmy

  2. Department of Environmental Biotechnology, City for Scientific Research and Technology Application, P.O. Box 21934, Alexandria, Egypt

    Hany Hussein & Mohamed Fahmy

Authors
  1. Ebtesam El Bestawy
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  2. Shacker Helmy
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  3. Hany Hussein
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  4. Mohamed Fahmy
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Correspondence to Ebtesam El Bestawy.

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El Bestawy, E., Helmy, S., Hussein, H. et al. Optimization and/or acclimatization of activated sludge process under heavy metals stress. World J Microbiol Biotechnol 29, 693–705 (2013). https://doi.org/10.1007/s11274-012-1225-9

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  • DOI: https://doi.org/10.1007/s11274-012-1225-9

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