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Physiological and enzymatic comparison between Pichia stipitis and recombinant Saccharomyces cerevisiae on xylose fermentation

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Abstract

In order to better understand the differences in xylose metabolism between natural xylose-utilizing Pichia stipitis and metabolically engineered Saccharomyces cerevisiae, we constructed a series of recombinant S. cerevisiae strains with different xylose reductase/xylitol dehydrogenase/xylulokinase activity ratios by integrating xylitol dehydrogenase gene (XYL2) into the chromosome with variable copies and heterogeneously expressing xylose reductase gene (XYL1) and endogenous xylulokinase gene (XKS1). The strain with the highest specific xylose uptake rate and ethanol productivity on pure xylose fermentation was selected to compare to P. stipitis under oxygen-limited condition. Physiological and enzymatic comparison showed that they have different patterns of xylose metabolism and NADPH generation.

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Abbreviations

XR:

Xylose reductase

XDH:

Xylitol dehydrogenase

XK:

Xylulokinase

G6PDH:

Glucose-6-phosphate dehydrogenase

IDP:

Isocitrate dehydrogenase

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Acknowledgments

This study was supported by the National Basic Research Program of China (No. 2004CB719702) and the National Knowledge Innovation Project of the Chinese Academy of Sciences. We thank Dr. Blanca Valle for reading and revising the manuscript.

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Authors and Affiliations

  1. Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China

    Changying Guo & Ning Jiang

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  1. Changying Guo
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  2. Ning Jiang
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Correspondence to Changying Guo.

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Guo, C., Jiang, N. Physiological and enzymatic comparison between Pichia stipitis and recombinant Saccharomyces cerevisiae on xylose fermentation. World J Microbiol Biotechnol 29, 541–547 (2013). https://doi.org/10.1007/s11274-012-1208-x

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  • DOI: https://doi.org/10.1007/s11274-012-1208-x

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