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Biosynthesis of nicotinic acid from 3-cyanopyridine by a newly isolated Fusarium proliferatum ZJB-09150

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Abstract

In this study, nitriles were used as sole sources of nitrogen in the enrichments to isolate nitrile-converting microorganisms. A novel fungus named ZJB-09150 possessing nitrile-converting enzymes was obtained with 3-cyanopyridine as sole source of nitrogen, which was identified by morphology, biology and 18S rDNA gene sequence as Fusarium proliferatum. It was found that F. proliferatum had ability to convert nitriles to corresponding acids or amides and showed wide substrate specificity to aliphatic nitriles, aromatic nitriles and ortho-substituted heterocyclic nitriles. The nitrile converting enzymes including nitrilase and nitrile hydratase in ZJB-09150 were induced by ε-caprolactam. Nitrilase obtained in this study showed high activity toward 3-cyanopyridine. It was active within pH 3.0–12.0 and temperature ranging from 25 to 65 °C with optimal at pH 9.0 and temperature 50–55 °C. The enzyme was thermostable and its half-life was 12.5 and 6 h at 45 and 55 °C, respectively. Under optimized reaction conditions, 60 mM 3-cyanopyridine was converted to nicotinic acid in 15 min, which indicated ZJB-09150 has potentials of application in large scale production of nicotinic acid.

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Acknowledgments

The authors gratefully acknowledge the Major Basic Research Development Program of China (973 Project) (No. 2011CB710806), the Research Program of Science and Technology Department of Zhejiang Province (No. 2011R09043-07) and the Zhejiang Province Natural Sciences Foundation of China (No. Y4080334, Y3110391, Y4110409).

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Authors and Affiliations

  1. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Li-Qun Jin, Zhi-Qiang Liu, Jian-Miao Xu & Yu-Guo Zheng

  2. Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, 310014, People’s Republic of China

    Li-Qun Jin, Zhi-Qiang Liu, Jian-Miao Xu & Yu-Guo Zheng

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  1. Li-Qun Jin
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  2. Zhi-Qiang Liu
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  3. Jian-Miao Xu
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  4. Yu-Guo Zheng
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Correspondence to Yu-Guo Zheng.

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Jin, LQ., Liu, ZQ., Xu, JM. et al. Biosynthesis of nicotinic acid from 3-cyanopyridine by a newly isolated Fusarium proliferatum ZJB-09150. World J Microbiol Biotechnol 29, 431–440 (2013). https://doi.org/10.1007/s11274-012-1195-y

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  • DOI: https://doi.org/10.1007/s11274-012-1195-y

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