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Evaluation of the bioactivities of extracts of endophytes isolated from Taiwanese herbal plants

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Abstract

The endophytic extracts from 19 endophytes, isolated from 13 species of Taiwanese plants, were evaluated for biological activity, including cytotoxicity, anti-platelet aggregation, and anti-inflammatory activity. The extracts of 12 endophytes exhibited inhibitory effects on collagen-induced platelet aggregation with IC50 values of 19.85–87.64 μg/ml. Four strains, Rahnella aquatilis, Pantoea agglomerans, Rhodotorula sp., and Penicillium paxilli, also showed inhibitory effects on thrombin-induced platelet aggregation with IC50 values of 42.80–61.54 μg/ml. Additionally 12 extracts of endophytes exhibited cytotoxicities with IC50 values of 0.12–19.83 μg/ml. However, eight extracts revealed inhibitory effects on superoxide anion generation induced by fMLP (N-formyl-l-methionyl-l-leucyl-l-phenylalanine) in human neutrophils. The extract of Rahnella aquatilis showed anti-platelet aggregation activity, and bioassay-directed fractionation led to the isolation of six compounds, including one isoalloxazine: lumichrome (1); two isoflavones: genistein (2) and daidzein (3); two cyclic peptides: cyclo-Pro-Val (4) and cyclo-Pro-Phe (5); and one benzenoid: methyl 2,4,5-trimethoxybenzoate (6). These results indicated that endophytes from Taiwanese herbal plants could be useful sources for research and development of bioactive lead compounds from nature.

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Acknowledgments

We gratefully acknowledge financial support for this project from the Kaohsiung Medical University (Q-9505) and Chang Gung University (CMRPD-170431). The authors also thank the Bioresource Collection and Research Centre for support in identification of endophytes.

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Authors and Affiliations

  1. Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan, ROC

    Pei-Wen Hsieh & Tsong-Long Hwang

  2. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC

    Pei-Wen Hsieh, Li-Chi Hsu, Chin-Chung Wu & Yang-Chang Wu

  3. Graduate Institute of Dental Sciences, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC

    Chern-Hsiung Lai

  4. Graduate Institute of Clinical Medical Sciences, Chang Gung University, Kweisan, Taoyuan, Taiwan, ROC

    Yin-Ku Lin

  5. Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan, ROC

    Yin-Ku Lin

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  1. Pei-Wen Hsieh
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Hsieh, PW., Hsu, LC., Lai, CH. et al. Evaluation of the bioactivities of extracts of endophytes isolated from Taiwanese herbal plants. World J Microbiol Biotechnol 25, 1461–1469 (2009). https://doi.org/10.1007/s11274-009-0036-0

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