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Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis

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Abstract

Phytophthora blight is one of the most important devastating diseases of red pepper plants. Forty-one bacterial isolates were obtained from rhizosphere soil and subsequently tested for antagonistic activity under in vitro and in vivo conditions. Among the 41 isolates tested, 12 exhibited a maximum antagonistic activity in dual culture assay. These 12 isolates were further screened for disease suppression on red pepper plants in both natural and greenhouse conditions. All the antagonists showed varying levels of antagonism, whereas the isolates R33 and R13 exhibited the maximum (86.8 and 71%) ability to reduce the disease severity in in vivo conditions. Based on the 16S rDNA sequencing, the most effective isolates were identified as Bacillussubtilis. In addition, the isolates were also screened for siderophores, hydrogen cyanide and hydrolytic enzymes. Further, the isolates increased the root and shoot length of the red pepper, which is an added advantage of the isolates while performing the desired function.

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Acknowledgement

This work was supported by Grant R01-2006-000-10491-0 from Basic Research Program of the KOSEF and Chonbuk National University Grant 2006. Author (S.K.K) is grateful to the Chonbuk National University for the Postdoctoral grant 2007. Authors wish to thank the anonymous reviewers for constructive comments.

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Authors and Affiliations

  1. Division of Bioresources Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, South Korea

    Kui Jae Lee & Gun Woong Lee

  2. Research Institute of Bioindustry, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, South Korea

    Seralathan Kamala-Kannan

  3. Division of Forest Science, College of Agriculture and Life Science, Chonbuk National University, Jeonju, 561-756, South Korea

    Han Sang Sub

  4. Division of Science Education, Chonbuk National University, Jeonju, 561-756, South Korea

    Cho Kyu Seong

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  1. Kui Jae Lee
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  2. Seralathan Kamala-Kannan
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  3. Han Sang Sub
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  4. Cho Kyu Seong
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  5. Gun Woong Lee
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Correspondence to Seralathan Kamala-Kannan.

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Lee, K.J., Kamala-Kannan, S., Sub, H.S. et al. Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis . World J Microbiol Biotechnol 24, 1139–1145 (2008). https://doi.org/10.1007/s11274-007-9585-2

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  • DOI: https://doi.org/10.1007/s11274-007-9585-2

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