Abstract
Intermediate products from anaerobic fermentation, such as volatile fatty acids (VFA), are the preferred carbon sources for the production of added-value products, namely polyhydroxyalkanoates (PHA) or bioenergy. Organic fraction of municipal solid waste (OFMSW) can be valorized through the application of a hydrolytic-acidogenic stage, thus reducing its pollutant content and at the same time that it is obtaining high-value products (VFA). In this work, the anaerobic fermentation of OFMSW into VFA (production and profile) and the influence of both total solids (TS) content in the reactor and alkalinity addition were studied. The increase on TS content led to a decrease on the acidification degree whereas the increase on the alkalinity addition led to a higher degree of acidification. Hence, the highest degree of acidification (77.59 %) was obtained at the lowest TS content (5 %) and at the highest alkalinity addition (50 g CaCO3 L−1). However, depending on the ultimate use of the produced VFA, the acidified residue presenting the highest VFA content (98.96 %) with higher propionic acid concentration, which is a more suitable VFA mixture for the production of high-quality PHA, was obtained at an intermediate TS content (8 %). From the response surfaces obtained, it was observed that all response variables (VFA production, degree of acidification, and effluent quality) presented a higher dependency on TS content than on initial alkalinity addition.
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Albuquerque, M. G., Eiroa, M., Torres, C., Nunes, B. R., & Reis, M. A. M. (2007). Strategies for the development of a side stream process for polyhydroxyalkanoate (PHA) production from sugar cane molasses. Journal of Biotechnology, 130(4), 411–421.
Albuquerque, M. G., Martino, V., Pollet, E., Avérous, L., & Reis, M. A. M. (2011). Mixed culture polyhydroxyalkanoate (PHA) production from volatile fatty acid (VFA)-rich streams: effect of substrate composition and feeding regime on PHA productivity, composition and properties. Journal of Biotechnology, 151(1), 66–76.
APHA, AWWA, & WEF. (2005). Standard methods for the examination of water and wastewater (21st ed.). Washington DC: American Public Health Association.
Appels, L., Baeyens, J., Degréve, J., & Dewil, R. (2008). Principles and potential of the anaerobic digestion of waste-activated sludge. Progress in Energy and Combustion Science, 34, 755–781.
Aytar, P., Gedikli, S., Sam, M., Farizoglu, B., & Çabuk, A. (2013). Sequential treatment of olive oil mill wastewater with adsorption and biological and photo-Fenton oxidation. Environmental Science and Pollution Research, 20, 3060–3067.
Bengtsson, S., Hallquist, J., Werker, A., & Welander, T. (2008). Acidogenic fermentation of industrial wastewaters: effects of chemostat retention time and pH on VFA production. Biochemical Engineering Journal, 40, 492–499.
Bertanza, G., Galessi, R., Menoni, L., Pedrazzani, R., Salvetti, R., & Zanaboni, S. (2015). Anaerobic treatability of liquid residue from wet oxidation of sewage sludge. Environmental Science and Pollution Research, 22, 7317–7326.
Bouallagui, H., Touhami, Y., Ben Cheikh, R., & Hamdi, M. (2005). Bioreactor performance in anaerobic digestion of fruit and vegetable wastes. Process Biochemestry, 40(3-4), 989–995.
Cai, M., Chua, H., Zhao, Q., Sin, N. S., & Ren, J. (2009). Optimal production of polyhydroxyalkanoates (PHA) in activated sludge fed by volatile fatty acids (VFAs) generated from alkaline excess sludge fermentation. Bioresource Technology, 100, 1399–1405.
Capela, I., Rodrigues, A., Silva, F. C., Nadais, H., & Arroja, L. (2008). Impact of industrial sludge and cattle manure on anaerobic digestion of the OFMSW under mesophilic conditions. Biomass and Bioenergy, 32(3), 245–251.
Chen, Y. G., Li, X., Zheng, X., & Wang, D. B. (2013). Enhancement of propionic acid fraction in volatile fatty acids produced from sludge fermentation by the use of food waste and Propionibacterium acidipropionici. Water Research, 47(2), 615–622.
Cropper, T. E., & Hanna, E. (2014). An analysis of the climate of Macaronesia, 1865-2012. International Journal of Climatology, 34, 604–622.
Dahiya, S., Sarkar, O., Swamy, Y. V., & Venkata Mohan, S. (2015). Acidogenic fermentation of food waste for volatile fatty acid production with co-generation of biohydrogen. Bioresource Technology, 182, 103–113.
Di Maria, F., & Micale, C. (2015). Life cycle analysis of management options for organic waste collected in an urban area. Environmental Science and Pollution Research, 22, 248–263.
Dionisi, D., Carucci, G., Papini, M. P., Riccardi, C., Majone, M., & Carrasco, F. (2005). Olive oil mill effluents as a feedstock for production of biodegradable polymers. Water Research, 39, 2076–2084.
Dogan, E., Tunaev, T., Erguder, T. H., & Demirer, G. N. (2008). Performance of leaching bed reactor converting the organic fraction of municipal solid waste to organic acids and alcohols. Chemosphere, 74, 797–803.
Doi, Y., Kunioka, M., Nakamura, Y., & Soga, K. (1987). Biosynthesis of copolyesters in Alcaligenes eutrophus H16 from carbon-13 labeled acetate and propionate. Macromolecules, 20(12), 2988–2991.
Fang, H. H., & Liu, H. (2002). Effect of pH on hydrogen production from glucose by a mixed culture. Bioresource Technology, 82, 87–93.
Fdez-Güelfo, L. A., Alvarez-Gallego, C., Márquez, D. S., & García, L. I. R. (2011). Biological pretreatment applied to industrial organic fraction of municipal solid wastes (OFMSW): effect on anaerobic digestion. Chemical Engineering Journal, 172, 321–325.
Fontanille, P., Kumar, V., Christophe, G., Nouaille, R., & Larroche, C. (2012). Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica. Bioresource Technology, 114, 443–449.
Gameiro, T., Sousa, F., Silva, F. C., Couras, C., Louros, V., Nadais, H., & Capela, I. (2015). Olive oil wastewater to volatile fatty acids: statistical study of the acidogenic process. Water, Air, & Soil Pollution, 226, 115.
Gavala, H. N., Yenal, U., Skiadas, I. V., Westermann, P., & Ahring, B. K. (2003). Mesophilic and thermophilic anaerobic digestion of primary and secondary sludge: effect of pre-treatment at elevated temperature. Water Research, 37, 4561–4572.
Giudicianni, P., Bozza, P., Sorrentino, G., & Ragucci, R. (2015). Thermal and mechanical stabilization process of the organic fraction of the municipal solid waste. Waste Management, 44, 125–134.
INE, I. P. (2014). Estatísticas do Ambiente 2013. Lisboa.
Jankowska, E., Chwialkowska, J., Stodolny, M., & Oleskowicz-Popiel, P. (2015). Effect of pH and retention time on volatile fatty acids production during mixed culture fermentation. Bioresource Technology, 190, 274–280.
Jiang, W. Z., Kitamura, Y., & Li, B. (2005). Improving acidogenic performance in anaerobic degradation of solid organic waste using a rotational drum fermentation system. Bioresource Technology, 96, 1537–43.
Jiang, Y. M., Chen, Y. G., & Zheng, X. (2009). Efficient polyhydroxyalkanoates production from a waste-activated sludge alkaline fermentation liquid by activated sludge submitted to the aerobic feeding and discharge process. Environmental Science & Technology, 43, 7734–7741.
Jiang, Y., Marang, L., Tamis, J., van Loosdrecht, M. C. M., Dijkman, H., & Kleerebezem, R. (2012). Waste to resource: converting paper mill wastewater to bioplastic. Water Research, 46(17), 5517–5530.
Kim, J., Park, C., Kim, T. H., Lee, M., Kim, S., Kim, S. W., & Lee, J. (2003). Effects of various pretreatments for enhanced anaerobic digestion with waste activated sludge. Journal of Bioscience and Bioengineering, 95, 271–275.
Koutinas, A. A., Vlysidis, A., Pleissner, D., Kopsahelis, N., Garcia, I. L., Kookos, I. K., Papanikolaou, S., Kwanb, T. H., & Lin, C. S. K. (2014). Valorization of industrial waste and by-product streams via fermentation for the production of chemicals and biopolymers. Chemical Society Reviews, 43, 2587–2627.
Lau, N.-S., Chee, J. Y., Tsuge, T., & Sudesh, K. (2010). Biosynthesis and mobilization of a novel polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer produced by Burkholderia sp. USM (JCM15050). Bioresource Technology, 101(20), 7916–7923.
Le Hyaric, R., Benbelkacem, H., Bollon, J., Bayard, R., Escudié, R., & Buffière, P. (2012). Influence of moisture content on the specific methanogenic activity of dry mesophilic municipal solid waste digestate. J Chemical Technology and Biotechnology, 87, 1032–1035.
Lee, W. S., Chua, A. S. M., Yeoh, H. K., & Ngoh, G. C. (2014). A review of the production and applications of waste-derived volatile fatty acids. Chemical Engineering Journal, 235, 83–99.
Li, Y., Park, S. Y., & Zhu, J. (2011). Solid-state anaerobic digestion for methane production from organic waste. Renewable and Sustainable Energy Reviews, 15(1), 821–826.
Lim, S., Kim, B. K., Jeong, C., Choi, J., Ahn, Y. H., & Chang, H. N. (2008). Anaerobic organic acid production of food waste in once-a-day feeding and drawing-off bioreactor. Bioresource Technology, 99(16), 7866–7874.
Liotta, F., Chatellier, P., Esposito, G., Fabbricino, M., Frunzo, L., van Hullebusch, E. D., Lens, P. N. L., & Pirozzi, F. (2015). Modified anaerobic digestion model no. 1 for dry and semi-dry anaerobic digestion of solid organic waste. Environmental Technology, 36(7), 870–880.
Liu, H., Wang, J., Liu, X., Fu, B., Chen, J., & Yu, H. Q. (2012). Acidogenic fermentation of proteinaceous sewage sludge: effect pH. Water Research, 43, 799–807.
Malina, J. F., & Pohland, F. G. (1992). Design of anaerobic processes for the treatment of industrial and municipal wastes. Water Quality Management Library Vol., 7.
Marouani, L., Bouallagui, H., Ben Cheikh, R., & Hamdi, M. (2002). Biomethanation of green wastes of wholesale market of Tunis. In Proceedings of the International Symposium on Environmental Pollution Control and Waste Management, 7–10 January 2002 (pp. 318–23).
Matthies, C., & Schink, B. (1992). Reciprocal isomerization of butyrate and iso-butyrate by the strictly anaerobic bacterium strain WoG13 and methanogenic iso-butyrate degradation by a defined triculture. Applied and Environmental Microbiology, 58(5), 1435–1439.
Merlin Christy, P., Gopinath, L. R., & Divya, D. (2014). A review on anaerobic decomposition and enhancement of biogas production through enzymes and microorganisms. Renewable and Sustainable Energy Reviews, 34, 167–173.
Myers, R. H., Montgomery, D. C., & Anderson-Cook, C. (2009). Response surface methodology: process and product optimization using designed experiments. Series in probability and statistics (p. 704). New York: Wiley.
Pardelha, F., Albuquerque, M. G. E., Reis, M. A. M., Dias, H. M. L., & Oliveira, R. (2012). Flux balance analysis of mixed microbial cultures: application to the production of polyhydroxyalkanoates from complex mixtures of volatile fatty. Journal of Biotechnology, 162, 336–345.
Park, W. J., Ahn, J. H., Hwang, S., & Lee, C. K. (2010). Effect of output power, target temperature, and solid concentration on the solubilization of waste activated sludge using microwave irradiation. Bioresource Technology, 101(1), S13–S16.
Raposo, F., de la Rubia, M. A., Borja, R., & Alaiz, M. (2008). Assessment of a modified and optimised method for determining chemical oxygen demand of solid substrates and solutions with high suspended solid content. Talanta, 76, 448–453.
Rodriguéz-Pimentel, R. I., Rodriguéz-Pérez, S., Monroy-Hermosillo, O., & Ramírez-Vives, F. (2015). Effect of organic loading rate on the performance of two-stage anaerobic digestion of the organic fraction of municipal solid waste (OFMSW). Water Science & Technology, 72(3), 384–390.
Romano, R. T., & Zhang, R. (2008). Co-digestion of onion juice and wastewater sludge using an anaerobic mixed biofilm reactor. Bioresource Technology, 99(3), 631–637.
Silva, F. C., Serafim, L. S., Nadais, H., Arroja, L., & Capela, I. (2013). Acidogenic fermentation towards valorisation of organic waste streams into volatile fatty acids. Chemical & Biochemical Engineering Quarterly, 27(4), 467–476.
Silvestre, G., Bonmatí, A., & Fernández, B. (2015). Optimisation of sewage sludge anaerobic digestion through co-digestion with OFMSW: effect of collection system and particle size. Waste Management, 43, 137–143.
Singh, M., Kumar, P., Ray, S., & Kalia, V. C. (2015). Challenges and opportunities for customizing polyhydroxyalkanoates. Indian Journal of Microbiology, 55(3), 235–249.
van Lier, B., Rebac, S., Lens, P., van Bijnen, F., Elferink, S., Stams, M., & Lettinga, G. (1997). Anaerobic treatment of partly acidified wastewater in a two-stage expanded granular sludge bed (EGSB) system at 8 degrees C. Water Science & Technology, 36(6–7), 317–324.
Vergine, P., Zábranská, J., & Canziani, R. (2014). Low temperature microwave and conventional heating pre-treatments to improve sludge anaerobic biodegradability. Water Science & Technology, 69(3), 518–524.
Vergine, P., Sousa, F., Lopes, M., Silva, F. C., Gameiro, T., Nadais, H., & Capela, I. (2015). Synthetic soft drink wastewater suitability for the production of volatile fatty acids. Process Biochemistry, 50, 1308–1312.
Wang, Q. H., Kuninobu, M., Ogawa, H. I., & Kato, Y. (1999). Degradation of volatile fatty acids in highly efficient anaerobic digestion. Biomass and Bioenergy, 16(6), 407–416.
Wang, K., Yin, J., Shen, D., & Li, N. (2014). Anaerobic digestion of food waste for volatile fatty acids (VFAs) production with different types of inoculum: Effect of pH. Bioresource Technology, 161, 395–401.
Wu, M., Sun, K., & Zhang, Y. (2006). Influence of temperature fluctuation on thermophilic anaerobic digestion of municipal organic solid waste. Journal of Zhejiang University SCIENCE B, 7(3), 180–185.
Xing, Y., Li, Z., Fan, Y., & Hou, H. (2010). Biohydrogen production from dairy manures with acidification pretreatment by anaerobic fermentation. Environmental Science and Pollution Research, 17, 392–399.
Xu, S. Y., Karthikeyan, O. P., Selvam, A., & Wong, J. W. C. (2012). Effect of inoculum to substrate ratio on the hydrolysis and acidification of food waste in leach bed reactor. Bioresource Technology, 126, 425–430.
Xue, Y., Liu, H., Chen, S., Dichtl, N., Dai, X., & Li, N. (2015). Effects of thermal hydrolysis on organic matter solubilization and anaerobic digestion of high solid sludge. Chemical Engineering Journal, 264, 174–180.
Yu, H. Q., Fang, H. H. P., & Gu, G. W. (2002). Comparative performance of mesophilic and thermophilic acidogenic upflow reactors. Process Biochemistry, 38(3), 447–454.
Zhen, G., Lu, X., Li, Y., & Zhao, Y. (2014). Combined electrical-alkali pretreatment to increase the anaerobic hydrolysis rate of waste activated sludge during anaerobic digestion. Applied Energy, 128, 93–102.
Zsigraiová, Z., Tavares, G., Semiao, V., & Carvalho, M. D. G. (2009). Integrated waste-to-energy conversion and waste transportation within island communities. Energy, 34, 623–635.
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Thanks are due, for the financial support to CESAM (UID/AMB/50017), to FCT/MEC through national funds (project PTDC/AMB-AAC/111316/2009), and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. M Lopes acknowledges her Ph.D. grant (153/CG/DAFII/NB/21371/2011) from CAMOES – Instituto da Cooperação e da Língua.
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Tânia Gameiro and Maria Lopes contributed equally to this work.
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Gameiro, T., Lopes, M., Marinho, R. et al. Hydrolytic-Acidogenic Fermentation of Organic Solid Waste for Volatile Fatty Acids Production at Different Solids Concentrations and Alkalinity Addition. Water Air Soil Pollut 227, 391 (2016). https://doi.org/10.1007/s11270-016-3086-6
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DOI: https://doi.org/10.1007/s11270-016-3086-6