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Complete nucleotide sequences of dsRNA2 and dsRNA7 detected in the phytopathogenic fungus Sclerotium hydrophilum and their close phylogenetic relationship to a group of unclassified viruses

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Abstract

Ten dsRNA segments were extracted from Sclerotium hydrophilum isolate (HZ11). The isolation of virus-like particles contained 10 dsRNA segments with the same number and migration as those extracted directly from the fungal mycelia. Two of these dsRNA segments, dsRNA2 and dsRNA7, were cloned and sequenced. They were 2121 and 1953 bp, respectively. The dsRNA2 encodes a RNA-dependent RNA polymerase. The dsRNA7 contains two open reading frames that encode putative proteins of unknown functions. Phylogenetic analysis of the putative proteins indicated that they are closely related to protein encoded by unclassified viruses, such as Cryphonectria parasitica bipartite mycovirus 1, Lactarius rufus RNA virus 1, Penicillium aurantiogriseum bipartite virus 1, and Curvularia thermal tolerance virus. The 5′- and 3′-untranslated regions of the two dsRNAs share significant sequence identity and contain conserved sequence stretches. It suggested that dsRNA2 and dsRNA7 have a common origin and a close phylogenetic relationship to a group of unclassified viruses.

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Acknowledgments

We thank Dr. J B Chen from National Cancer Institute (USA) and Dr. Yu Xiang from Agriculture and Agri-Food (Canada) for editing the manuscript. This research was supported by the National Natural Science Foundation of China (No. 31071729), the Key Project of the Education Department of Hunan Province (No. 4A075), the Natural Science Fund of Hunan Province (No. 2015JJ2084), and Hunan Provincial Collaborative Innovation Center for Farmland Weeds Control.

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Correspondence to Xiwu Zhu.

Additional information

Edited by Thomas Hohn.

The GenBank Accession numbers of the sequences reported in this article are KU886558 and KU886559.

Electronic supplementary material

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11262_2016_1375_MOESM1_ESM.tif

The electrophoresis of dsRNA isolates from other S. hydrophilum. These isolates were not treated by DNase I. Lane 1, Mark; Lane 2, HZ#1; Lane 3, HZ#2; Lane 4, HZ#3; Lane 5, HZ#4; Lane 6, HZ#5; Lane 7, HZ#6; Lane 8, HZ#7. Supplementary material 1 (TIFF 900 kb)

11262_2016_1375_MOESM2_ESM.tif

The amino acid sequence identity of dsRNA2-P with other related virus and virus-like sequences. Upper triangle region: percent identity. dsRNA protein was DsRNA2 protein; AGK89731.1, RdRp of Cryphonectria parasitica bipartite mycovirus 1; AMK47911.1, RdRp of Lactarius rufus RNA virus 1; AKD01578.1, RdRp of Rhizoctonia fumigata mycovirus; AFZ85210.1, RdRp of Rhizoctonia solani dsRNA virus 1; KFH72371.1, hypothetical protein MVEG 02662 of Mortierella verticillata NRRL 6337; AIU98624.1, RdRp of Gremmeniella abietina RNA virus 6; ALO50128.1, putative RdRp of Penicillium aurantiogriseum bipartite virus 1; ABM92658.1, putative replicase of Curvularia thermal tolerance virus; ALO61397.1, putative RdRp of Curvularia thermal tolerance virus; ALO61396.1, putative RdRp of Curvularia thermal tolerance virus; ALO61392.1, putative RdRp of Curvularia virus 2; ALO61391.1, putative RdRp of Curvularia virus 2; ALO61393.1, putative RdRp of Curvularia virus 2; ALO61390.1, putative RdRp of Curvularia virus 2; ACY56324.1, putative RdRp of of Fusarium graminearum dsRNA mycovirus-4; ADW82835.1, putative RdRp of Heterobasidion RNA virus 6; AHA82556.1, RdRp of Heterobasidion RNA virus 6; AHA82552.1, RdRp of Heterobasidion RNA virus 6; AEZ35182.2, putative RdRp of Heterobasidion RNA virus 6; ADW82833.1, putative RdRp of Heterobasidion RNA virus 6; AHA82546.1, RdRp of Heterobasidion RNA virus 6; ABM92658.1, RdRp of Beauveria bassiana RNA virus 1. Supplementary material 2 (TIFF 2339 kb)

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Wang, C., Wu, J., Zhu, X. et al. Complete nucleotide sequences of dsRNA2 and dsRNA7 detected in the phytopathogenic fungus Sclerotium hydrophilum and their close phylogenetic relationship to a group of unclassified viruses. Virus Genes 52, 823–827 (2016). https://doi.org/10.1007/s11262-016-1375-1

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