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CpG methylation in human papillomavirus (HPV) type 31 long control region (LCR) in cervical infections associated with cytological abnormalities

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Abstract

The mechanisms that regulate papillomavirus gene expression include DNA methylation. The transcription of papillomavirus oncogenes E6 and E7 is controlled by certain regulatory elements in the LCR, which include binding sites for the E2 protein, a viral regulator of oncogene expression. In HPV-31-infected exfoliated cervical cells, the CpG methylation of the entire LCR was determined by next-generation sequencing after bisulfite modification. Six of the 22 cases had methylated CpG sites in the HPV-31 LCR, including position 7479 and/or 7485, at the promoter distal E2 binding site, thus suggesting a potential regulatory mechanism for papillomavirus transcription.

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Acknowledgments

Special thanks for Dr. Miranda Thomas for careful reading of the manuscript and improving its text. Eszter Gyöngyösi was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4. A/1-11-1-2012-0001 ‘National Excellence Program’. This study was supported by a grant from Hungarian Scientific Research Fund (OTKA K 81422).

Author contributions

József Kónya and György Veress conceived and designed the experiments. Brigitta László and László Madar performed the majority of experiments and analyzed data. Brigitta László and József Kónya prepared the manuscript. Annamária Ferenczi, Eszter Gyöngyösi, Anita Szalmás, and Levente Szakács participated in some of the experiments. All the authors assisted in writing the manuscript and approved the final manuscript.

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary

    Brigitta László, Annamária Ferenczi, Eszter Gyöngyösi, Anita Szalmás, Levente Szakács, György Veress & József Kónya

  2. Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary

    László Madar

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  1. Brigitta László
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  2. Annamária Ferenczi
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  8. József Kónya
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Corresponding author

Correspondence to Brigitta László.

Ethics declarations

HPV typing was performed as part of routine laboratory diagnosis. Analyzing the microorganism has not required formal consent.

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All authors declare that they have no competing interests.

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Edited by Hartmut Hengel.

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László, B., Ferenczi, A., Madar, L. et al. CpG methylation in human papillomavirus (HPV) type 31 long control region (LCR) in cervical infections associated with cytological abnormalities. Virus Genes 52, 552–555 (2016). https://doi.org/10.1007/s11262-016-1338-6

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  • DOI: https://doi.org/10.1007/s11262-016-1338-6

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