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Isolation of an unusual G26P[13] group A rotavirus strain from piglets with diarrhea in Brazil

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Abstract

This report describes the identification of an unusual G26P[13] genotype combination in a porcine group A rotavirus (RVA) strain. In a retrospective study, the VP7 (G type) and VP4 (P type) genes of porcine RVA Brazilian field strains identified in two diarrheic suckling piglets were amplified by RT-PCR and subjected to sequencing. The sequence analysis revealed the G26P[13] RVA genotype in one strain (BRA381) and G26P[X] in the other (BRA382). This study presents evidence of porcine RVA G26 genotype circulating in a Brazilian pig herd.

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Acknowledgments

This study was supported by the following Brazilian institutions: the National Council for Scientific and Technological Development (CNPq) and the Araucaria Foundation (FAP/PR). Alfieri, A.A., Alfieri, A.F., and Lorenzetti, E. are recipients of CNPq fellowships.

Author contributions

EL and AAA participated in the design of the study. EL contributed in the laboratory work, data analysis and wrote the manuscript. AFA and AAA contributed to write the manuscript, overall supervision, and coordinated all of the laboratory procedures. All authors reviewed and approved the final manuscript.

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Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Campus Universitário, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil

    Elis Lorenzetti, Alice Fernandes Alfieri & Amauri Alcindo Alfieri

  2. Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Elis Lorenzetti, Alice Fernandes Alfieri & Amauri Alcindo Alfieri

Authors
  1. Elis Lorenzetti
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  2. Alice Fernandes Alfieri
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  3. Amauri Alcindo Alfieri
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Corresponding author

Correspondence to Amauri Alcindo Alfieri.

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Ethical approval

The study was submitted to Ethics Committee on Animal Experiments of the Universidade Estadual de Londrina and approved under the identification number 6371.2013.43. All applicable institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Edited by Dr.William Dundon.

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Lorenzetti, E., Alfieri, A.F. & Alfieri, A.A. Isolation of an unusual G26P[13] group A rotavirus strain from piglets with diarrhea in Brazil. Virus Genes 52, 586–591 (2016). https://doi.org/10.1007/s11262-016-1336-8

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  • DOI: https://doi.org/10.1007/s11262-016-1336-8

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