Skip to main content
SpringerLink
Log in

Analysis of watermelon chlorotic stunt virus and tomato leaf curl Palampur virus mixed and pseudo-recombination infections

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Watermelon chlorotic stunt virus (WmCSV) and tomato leaf curl Palampur virus (ToLCPMV) are limiting factors for cucurbit production in south and southeastern Iran. ToLCPMV infects all cucurbit crops (except watermelons) whereas WmCSV is somewhat limited to watermelon, causing detrimental effects on fruit production. In a survey, we detected WmCSV in all watermelon growing farms in Fars province (southern Iran). Given that WmCSV and ToLCPMV are present in the same geographical location in Iran, we studied the interaction of two viruses. Co-infection using agroinfectious clones of WmCSV and ToLCPMV caused severe symptoms in watermelon and zucchini in comparison to symptoms observed from individual infections. Interestingly, inoculation of zucchini with WmCSV DNA-A and ToLCPMV DNA-B agroinfectious clones or vice versa produced a viable pseudo-recombinant and induced systemic symptoms. This demonstrates that replication-associated protein of DNA-A of each virus is able to bind to cis elements of the DNA-B molecules of another virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. M.A. Al-Saleh, M.H. Ahmad, I.M. Al-Shahwan, J.K. Brown, A.M. Idris, Plant Dis. 98, 1451 (2014)

    Article  Google Scholar 

  2. E.C. Andrade, G.G. Manhani, P.F. Alfenas, R.F. Calegario, E.P.B. Fontes, F.M. Zerbini, J. Gen. Virol. 87, 3687–3696 (2006)

    Article  CAS  PubMed  Google Scholar 

  3. G.R. Arguello-Astorga, R. Ruiz-Medrano, Arch. Virol. 146, 465–485 (2001)

    Google Scholar 

  4. K. Bananej, A. Ahoonmanesh, A. Kheyr-Pour, J. Phytopathol. 150, 423–430 (2002)

    Article  Google Scholar 

  5. K. Bananej, A. Kheyr-Pour, A. Ahoonmanesh, in Proceeding of the 13th Iranian Plant Protection Congress, Karaj, Iran, p. 194 (1998)

  6. P. Bernardo, M. Golden, M. Akram, Naimuddin, N. Nadarajan, E. Fernandez, M. Granier, A.G. Rebelo, M. Peterschmitt, D.P. Martin, P. Roumagnac, Virus Res. 177, 35–45 (2013)

    Article  CAS  PubMed  Google Scholar 

  7. B. Böttcher, S. Unseld, H. Ceulemans, R.B. Russell, H. Jeske, J. Virol. 78, 6758–6765 (2004)

    Article  PubMed Central  PubMed  Google Scholar 

  8. J.K. Brown, C.M. Fauquet, R.W. Briddon, M. Zerbini, E. Moriones, J. Navas-Castillo, in Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses, ed. by A.M.Q. King, M.J. Adams, E.B. Carstens, E.J. Lefkowitz (Elsevier Inc, London, 2012), pp. 351–373

    Google Scholar 

  9. J.K. Brown, A.M. Idris, C. Alteri, D.C. Stenger, Phytopathology 92, 734–742 (2002)

    Article  CAS  PubMed  Google Scholar 

  10. J.K. Brown, A.M. Idris, K.M. Ostrow, N. Goldberg, R. French, D.C. Stenger, Phytopathology 95, 1217–1224 (2005)

    Article  CAS  PubMed  Google Scholar 

  11. J.K. Brown, F.M. Zerbini, J. Navaz-Castillo, E. Moriones, R. Ramos-Sobrinho, J.C.F. Silva, E. Fiallo-Olivé, R.W. Briddon, C. Hernández-Zepeda, A. Idris, V.G. Malathi, D.P. Martin, R. Rivera-Bustamante, S. Ueda, A. Varsani, Arch. Virol. 160, 1593–1619 (2015)

    Article  CAS  PubMed  Google Scholar 

  12. S. Chakraborty, R. Vanitharani, B. Chattopadhyay, C.M. Fauquet, J. Gen. Virol. 89, 818–828 (2008)

    Article  CAS  PubMed  Google Scholar 

  13. R.D. Edgar, Nucleic Acids Res. 32, 1792–1797 (2004)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. R. Fazeli, J. Heydarnejad, H. Massumi, M. Shaabanian, A. Varsani, Virus Genes 38, 311–319 (2009)

    Article  CAS  PubMed  Google Scholar 

  15. E.P. Fontes, P.A. Eagle, P.S. Sipe, V.A. Luckow, L. Hanley-Bowdoin, J. Biol. Chem. 269, 8459–8465 (1994)

    CAS  PubMed  Google Scholar 

  16. E.P. Fontes, H.J. Gladfelter, R.L. Schaffer, I.T.D. Petty, L. Hanley-Bowdoin, Plant Cell 6, 405–416 (1994)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. T. Frischmuth, S. Roberts, A. von Arnim, J. Stanley, Virology 196, 666–673 (1993)

    Article  CAS  PubMed  Google Scholar 

  18. M. Ghanim, Virus Res. 186, 47–54 (2014)

    Article  CAS  PubMed  Google Scholar 

  19. R.L. Gilbertson, S.H. Hidayat, E.J. Paplomatas, M.R. Rojas, Y.-M. Hou, D.P. Maxwell, J. Gen. Virol. 74, 23–31 (1993)

    Article  CAS  PubMed  Google Scholar 

  20. N. Grimsley, B. Hohn, T. Hohn, R. Walden, Proc. Natl. Acad. Sci. USA 83, 3282–3286 (1986)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. D. Haible, S. Kober, H. Jeske, J. Virol. Methods 135, 9–16 (2006)

    Article  CAS  PubMed  Google Scholar 

  22. J. Heydarnejad, M. Hesari, H. Massumi, A. Varsani, Aust. Plant Pathol. 42, 195–203 (2013)

    Article  Google Scholar 

  23. J. Heydarnejad, A. Mozaffari, H. Massumi, R. Fazeli, A.J.A. Gray, S. Meredith, F. Lakay, D.N. Shepherd, D.P. Martin, A. Varsani, Arch. Virol. 154, 1015–1018 (2009)

    Article  CAS  PubMed  Google Scholar 

  24. J.E. Hill, J.O. Strandberg, E. Hiebert, S.G. Lazarowitz, Virology 250, 283–292 (1998)

    Article  CAS  PubMed  Google Scholar 

  25. A.J. Khan, S. Akhtar, R.W. Briddon, U. Ammara, A.M. Al-Matrooshi, S. Mansoor, Viruses 4, 1169–1181 (2012)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. A. Kheyr-Pour, K. Bananej, G. Dafalla, P. Caciagli, E. Noris, A. Ahoonmanesh, H. Lecoq, B. Gronenborn, Phytopathology 90, 629–635 (2000)

    Article  CAS  PubMed  Google Scholar 

  27. B. Krenz, J.R. Thompson, M. Fuchs, K.L. Perry, J. Virol 86, 7715 (2012)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. M. Lapidot, D. Gelbart, A. GalOn, N. SelaM, G. Anfoka, F. HajAhmed, Y. AbouJawada, H. Sobh, H. Mazyad, A.A. AboulAta, A.K. El-Attar, M.S. AliShtayeh, R.M. Jamous, J.E. Polston, S. Duffy, J. Virol. 11, 181 (2014)

    Article  Google Scholar 

  29. S.G. Lazarowitz, Virology 180, 70–80 (1991)

    Article  CAS  PubMed  Google Scholar 

  30. G. Loconsole, P. Saldarelli, H. Doddapaneni, V. Savino, G.P. Martelli, M. Saponari, Virology 432, 162–172 (2012)

    Article  CAS  PubMed  Google Scholar 

  31. A.H. Malik, R.W. Briddon, M. Mansoor, Virol. J. 8, 173 (2011)

    Article  PubMed Central  PubMed  Google Scholar 

  32. D.P. Martin, P. Biagini, P. Lefeuvre, M. Golden, P. Roumagnac, A. Varsani, Viruses 3, 1699–1738 (2011)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. E. Moriones, S. García-Andrés, J. Navas-Castillo, in Tomato yellow leaf curl virus disease, management, molecular biology, breeding for resistance, ed. by H. Czosnek (Springer, Berlin, 2007), pp. 119–138

    Chapter  Google Scholar 

  34. B.M. Muhire, A. Varsani, D.P. Martin, PLoS ONE 9, 1–8 (2014)

    Article  Google Scholar 

  35. B.L. Patil, C.M. Fauquet, Virus Genes 50, 474–486 (2015)

    Article  CAS  PubMed  Google Scholar 

  36. B.L. Patil, N. Dutt, R. Briddon, S. Bull, D. Rothenstein, B.K. Borah, I. Dasgupta, J. Stanley, H. Jeske, Virus Res. 124, 59–67 (2007)

    Article  CAS  PubMed  Google Scholar 

  37. C.S. Rocha, G.P. Castillo-Urquiza, A.T.M. Lima, F.N. Silva, C.A.D. Xavier, B.T. Hora-Júnior, J.E.A. Beserra-Júnior, A.W.O. Malta, D.P. Martin, A. Varsani, P. Alfenas-Zerbini, E.S.G. Mizubuti, F.M. Zerbini, J. Virol. 87, 5784–5799 (2013)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  38. P. Roumagnac, M. Granier, P. Bernardo, M. Deshoux, R. Ferdinand, S. Galzi, E. Fernandez, C. Julian, I. Abt, D. Filloux, F. Mesléard, A. Varsani, S. Blanc, D.P. Martin, M. Peterschmitt, J. Virol. 89, 9683–9688 (2015)

    Article  CAS  PubMed  Google Scholar 

  39. M. Sabouri, J. Heydarnejad, Iran. Plant Pathol. J. 49, 403–409 (2013)

    Google Scholar 

  40. D.P. Shepherd, D.P. Martin, P. Lefeuvre, A.L. Monjane, B.E. Owor, E.P. Rybicki, A. Varsani, J. Virol. Methods 149, 97–102 (2008)

    Article  CAS  PubMed  Google Scholar 

  41. J. Stanley, R. Townsend, S.J. Curson, J. Gen. Virol. 66, 1055–1061 (1985)

    Article  CAS  Google Scholar 

  42. T. Sufrin-Ringwald, M. Lapidot, Phytopathology 101, 281–289 (2011)

    Article  PubMed  Google Scholar 

  43. Y.K. Sung, R.H.A. Coutts, J. Gen. Virol. 76, 2809–2815 (1995)

    Article  CAS  PubMed  Google Scholar 

  44. J. Syller, Mol. Plant Pathol. 13, 204–216 (2012)

    Article  PubMed  Google Scholar 

  45. S. Unseld, M. Ringel, P. Höfer, M. Höhnle, H. Jeske, I.D. Bedford, P.G. Markham, T. Frischmuth, Arch. Virol. 145, 1449–1454 (2000)

    Article  CAS  PubMed  Google Scholar 

  46. A. Varma, V.G. Malathi, Ann. Appl. Biol. 142, 145–164 (2003)

    Article  CAS  Google Scholar 

  47. A. Varsani, J. Navaz-Castillo, E. Moriones, C. Hernández-Zepeda, A. Idris, J.K. Brown, F.M. Zerbini, D.P. Martin, Arch. Virol. 159, 2193–2203 (2014)

    Article  CAS  PubMed  Google Scholar 

  48. A. Varsani, D.N. Shepherd, A.L. Monjane, B.E. Owor, J.B. Erdmann, E.P. Rybicki, M. Peterschmitt, R.W. Briddon, P.G. Markham, S. Oluwafemi, O.P. Windram, P. Lefeuvre, J.M. Lett, D.P. Martin, J. Gen. Virol. 89, 2063–2074 (2008)

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  49. W. Zhang, N.H. Olson, T.S. Baker, L. Faulkner, M. Agbandje-McKenna, M.I. Boulton, J.W. Davies, R. McKenna, Virology 279, 471–477 (2001)

    Article  CAS  PubMed  Google Scholar 

  50. Y.P. Zhang, J.K. Uyemoto, B.C. Kirkpatrick, J. Virol. Methods 71, 45–50 (1998)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors wish to thank Plant Protection Management, Fars Province for providing facilities for collection of samples. This research work was supported by the Iran National Science Foundation (INSF) (Grant No. 92012168). AV is supported by the National Research Foundation of South Africa.

Author information

Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

    Maryam Esmaeili, Jahangir Heydarnejad & Hossain Massumi

  2. Structural Biology Research Unit, Division of Medical Biochemistry, Department of Clinical Laboratory Sciences, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa

    Arvind Varsani

  3. Department of Plant Pathology and Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32611, USA

    Arvind Varsani

  4. School of Biological Sciences, and Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand

    Arvind Varsani

Authors
  1. Maryam Esmaeili
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jahangir Heydarnejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hossain Massumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Arvind Varsani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jahangir Heydarnejad.

Additional information

Edited by Seung-Kook Choi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Esmaeili, M., Heydarnejad, J., Massumi, H. et al. Analysis of watermelon chlorotic stunt virus and tomato leaf curl Palampur virus mixed and pseudo-recombination infections. Virus Genes 51, 408–416 (2015). https://doi.org/10.1007/s11262-015-1250-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-015-1250-5

Keywords

Search

Navigation