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Complete genome sequence of a new recombinant echovirus 25 strain isolated from a neonatal patient with hand, foot, and mouth disease complicated by encephalitis in Beijing, China

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Abstract

Although human echovirus 25 (E-25), a type of the enterovirus B species, is implicated in aseptic meningitis, information on its gene structure, evolution, and virulence are limited. We report here the complete genome sequence of a novel recombinant E-25 strain (E25/2010/CHN/BJ) isolated from a neonate with hand, foot, and mouth disease complicated by encephalitis in Beijing, China in 2010. The complete viral genome consists of 7429 nucleotides (nts), including a 6585-nt open reading frame. Phylogenetic dendrogram based on VP1 gene regions revealed that this strain belonged to subgroup D4, which contains the other E-25 strains isolated from China in recent years. The difference in the amino acid sites (P130S, K/T135I) of the VP1 region may affect its immunogenicity. SimPlot and Bootscan analyses suggested that E25/2010/CHN/BJ is a recombination result of E-25 and Coxsackievirus B3 (CVB-3) strains. Our results would facilitate the study of the origin, evolution, and molecular epidemiology of E-25.

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Acknowledgments

This work was supported by a grant from the Science and Technology Project of Beijing, China (No. D131100005313016). Support was also provided by Collaborative Innovation Center of Infectious Diseases and Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, China.

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    Authors and Affiliations

    1. Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China

      Hongjie Li, Yixing Meng, Jinqiu Liang, Hongping Lu, Qi Wang, Pu Liang, Jinfeng Cao, Shun-ai Liu & Jun Cheng

    2. Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, 100015, China

      Hongjie Li, Yixing Meng, Jinqiu Liang, Hongping Lu, Qi Wang, Pu Liang, Jinfeng Cao, Shun-ai Liu & Jun Cheng

    3. Department of General Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China

      Hongjie Li

    4. Beijing Ditan Hospital, Peking University Teaching Hospital, Beijing, 100015, China

      Yixing Meng & Jun Cheng

    5. Division of Pediatrics, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China

      Lin Pang

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    1. Hongjie Li
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    Correspondence to Shun-ai Liu or Jun Cheng.

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    Edited by Paul Schnitzler.

    Hongjie Li and Yixing Meng have contributed equally to this work.

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    Li, H., Meng, Y., Pang, L. et al. Complete genome sequence of a new recombinant echovirus 25 strain isolated from a neonatal patient with hand, foot, and mouth disease complicated by encephalitis in Beijing, China. Virus Genes 50, 505–508 (2015). https://doi.org/10.1007/s11262-015-1186-9

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    • DOI: https://doi.org/10.1007/s11262-015-1186-9

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