Skip to main content

Advertisement

SpringerLink
Log in

Sequence and phylogenetic analysis of surface protein genes of emerging H9N2 influenza viruses isolated from poultry in two geographical regions of China

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Subtype H9N2 avian influenza viruses (AIVs) circulating in China have aroused increasing concerns for their impact on poultry and risk to public health. The present study was an attempt to elucidate the phylogenetic relationship of H9N2 AIVs in two geographically distinct regions of China where vaccination is routinely practiced. A total of 18 emerging H9N2 isolates were identified and genetically characterized. Phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes confirmed that the isolates belonged to the Y280 lineage. Based on the HA genes, the isolates were subdivided into two subgroups. The viruses from Zhejiang Province were clustered together in Group I, while the isolates from Guangdong Province were clustered together in Group II. Antigenic characterization showed that the tested viruses were antigenically different when compared to the current used vaccine strain. It was notable that 14 out of total 18 isolates had an amino acid exchange (Q→L) at position 216 (226 by H3 Numbering) in the receptor-binding site, which indicated that the virus had potential affinity of binding to human like receptor. These results suggest that the emerging viruses have potential risk to public health than previously thought. Therefore, continuous surveillance studies of H9N2 influenza virus are very important to the prognosis and control of future influenza pandemics.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. P. Pushko, T.M. Tumpey, F. Bu, J. Knell, R. Robinson, G. Smith, Vaccine 23, 5751–5759 (2005)

    Article  CAS  PubMed  Google Scholar 

  2. R.A. Fouchier, V. Munster, A. Wallensten, T.M. Bestebroer, S. Herfst, D. Smith, G.F. Rimmelzwaan, B. Olsen, A.D. Osterhaus, J. Virol. 79, 2814–2822 (2005)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. A.S. Arafa, N.M. Hagag, N. Yehia, A.M. Zanaty, M.M. Naguib, S.A. Nasef, Avian Dis. 56, 849–857 (2012)

    Article  PubMed  Google Scholar 

  4. I.H. Brown, J. Banks, R.J. Manvell, S.C. Essen, W. Shell, M. Slomka, B. Londt, D.J. Alexander, Dev. Biol. Basel 124, 45–50 (2006)

    CAS  PubMed  Google Scholar 

  5. N. Kishida, Y. Sakoda, M. Eto, Y. Sunaga, H. Kida, Arch. Virol. 149, 2095–2104 (2004)

    Article  CAS  PubMed  Google Scholar 

  6. Y. Guan, K.F. Shortridge, S. Krauss, R.G. Webster, Proc. Natl. Acad. Sci. USA 96, 9363–9367 (1999)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. P.J. Homme, B.C. Easterday, Avian Dis. 14, 66–74 (1970)

    Article  CAS  PubMed  Google Scholar 

  8. A.R. Homayounimehr, H. Dadras, A. Shoushtari, S.A. Pourbakhsh, Trop. Anim. Health Prod. 42, 1291–1297 (2010)

    Article  PubMed  Google Scholar 

  9. K. Xu, L. Ferreri, A. Rimondi, V. Olivera, M. Romano, H. Ferreyra, V. Rago, M. Uhart, H. Chen, T. Sutton, A. Pereda, D.R. Perez, Virus Res. 168, 41–47 (2012)

    Article  CAS  PubMed  Google Scholar 

  10. Y.K. Choi, H. Ozaki, R.J. Webby, R.G. Webster, J.S. Peiris, L. Poon, C. Butt, Y.H. Leung, Y. Guan, J. Virol. 78, 8609–8614 (2004)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. C.H. Lee, S.H. Byun, Y.J. Lee, I.P. Mo, Virus Genes 45, 38–47 (2012)

    Article  CAS  PubMed  Google Scholar 

  12. S. Nagarajan, K. Rajukumar, C. Tosh, V. Ramaswamy, K. Purohit, G. Saxena, P. Behera, B. Pattnaik, H.K. Pradhan, S.C. Dubey, Vet. Microbiol. 133, 154–163 (2009)

    Article  CAS  PubMed  Google Scholar 

  13. I. Davidson, I. Shkoda, N. Golender, S. Perk, K. Lapin, Y. Khinich, A. Panshin, Virus Genes 46, 255–263 (2013)

    Article  CAS  PubMed  Google Scholar 

  14. S. Shahsavandi, A.H. Salmanian, S.A. Ghorashi, S. Masoudi, M.M. Ebrahimi, Res. Vet. Sci. 93, 234–239 (2012)

    Article  CAS  PubMed  Google Scholar 

  15. Y. Huang, B. Hu, X. Wen, S. Cao, B.K. Gavrilov, Q. Du, M.I. Khan, X. Zhang, Virus Res. 151, 26–32 (2010)

    Article  CAS  PubMed  Google Scholar 

  16. P. Zhang, Y. Tang, X. Liu, W. Liu, X. Zhang, H. Liu, D. Peng, S. Gao, Y. Wu, L. Zhang, S. Lu, J. Virol. 83, 8428–8438 (2009)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. K.M. Butt, G.J. Smith, H. Chen, L.J. Zhang, Y.H. Leung, K.M. Xu, W. Lim, R.G. Webster, K.Y. Yuen, J.S. Peiris, Y. Guan, J. Clin. Microbiol. 43, 5760–5767 (2005)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Y.P. Lin, M. Shaw, V. Gregory, K. Cameron, W. Lim, A. Klimov, K. Subbarao, Y. Guan, S. Krauss, K. Shortridge, R. Webster, N. Cox, A. Hay, Proc. Natl. Acad. Sci. USA 97, 9654–9658 (2000)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. M. Soltanialvar, H. Shoushtari, M. Bozorgmehrifard, S. Charkhkar, F. Akbarnejad, Trop. Anim. Health Prod. 44, 419–425 (2012)

    Article  PubMed  Google Scholar 

  20. K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731–2739 (2011)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. S. Edwards, Dev. Biol. Basel 124, 159–162 (2006)

    CAS  PubMed  Google Scholar 

  22. W. Weis, J.H. Brown, S. Cusack, J.C. Paulson, J.J. Skehel, D.C. Wiley, Nature 333, 426–431 (1988)

    Article  CAS  PubMed  Google Scholar 

  23. Y. Zhang, Y. Yin, Y. Bi, S. Wang, S. Xu, J. Wang, S. Zhou, T. Sun, K.J. Yoon, Vet. Microbiol. 156, 285–293 (2012)

    Article  PubMed  Google Scholar 

  24. H. Yu, R.H. Hua, T.C. Wei, Y.J. Zhou, Z.J. Tian, G.X. Li, T.Q. Liu, G.Z. Tong, Vet. Microbiol. 131, 82–92 (2008)

    Article  CAS  PubMed  Google Scholar 

  25. R. Gao, B. Cao, Y. Hu, Z. Feng, D. Wang, W. Hu, J. Chen, Z. Jie, H. Qiu, K. Xu, X. Xu, H. Lu, W. Zhu, Z. Gao, N. Xiang, Y. Shen, Z. He, Y. Gu, Z. Zhang, Y. Yang, X. Zhao, L. Zhou, X. Li, S. Zou, Y. Zhang, L. Yang, J. Guo, J. Dong, Q. Li, L. Dong, Y. Zhu, T. Bai, S. Wang, P. Hao, W. Yang, J. Han, H. Yu, D. Li, G.F. Gao, G. Wu, Y. Wang, Z. Yuan, Y. Shu, N. Engl. J. Med. 368, 1888–1897 (2013)

    Article  CAS  PubMed  Google Scholar 

  26. Y.C. Chu, C.L. Cheung, C.Y. Hung Leung, L.L. Man Poon, H. Chen, J.S. Peiris, Y. Guan, Influenza Other Respir Viruses 5(Suppl 1), 68–71 (2011)

    PubMed  Google Scholar 

  27. K.M. Xu, G.J. Smith, J. Bahl, L. Duan, H. Tai, D. Vijaykrishna, J. Wang, J.X. Zhang, K.S. Li, X.H. Fan, R.G. Webster, H. Chen, J.S. Peiris, Y. Guan, J. Virol. 81, 10389–10401 (2007)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  28. Y. Sun, Y. Tan, K. Wei, H. Sun, Y. Shi, J. Pu, H. Yang, G.F. Gao, Y. Yin, W. Feng, D.R. Perez, J. Liu, J. Virol. 87, 2963–2968 (2013)

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  29. D.E. Stallknecht, S.M. Shane, Vet. Res. Commun. 12, 125–141 (1988)

    Article  CAS  PubMed  Google Scholar 

  30. M.N. Matrosovich, S. Krauss, R.G. Webster, Virology 281, 156–162 (2001)

    Article  CAS  PubMed  Google Scholar 

  31. F. Chen, Z.Q. Yan, J. Liu, J. Ji, S. Chang, D. Liu, J.P. Qin, J.Y. Ma, Y.Z. Bi, Q.M. Xie, Virus Genes 45, 69–75 (2012)

    Article  CAS  PubMed  Google Scholar 

  32. A.M. Butt, S. Siddique, M. Idrees, Y. Tong, Virol. J. 7, 319 (2010)

    Article  PubMed Central  PubMed  Google Scholar 

  33. D.H. Lee, C.S. Song, Clin. Exp. Vaccin. Res. 2, 26–33 (2013)

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by a grant from the Science and Technology Planning Project of Guangdong Province China (No. 2010B090301019, 2009B020201008).

Author information

Authors and Affiliations

  1. College of Animal Science, South China Agricultural University, Guangzhou, 510642, China

    Yu Xue, Jing-Lan Wang, Zhuan-Qiang Yan, Guang-Wei Li, Shun-Yan Chen, Xiang-Bin Zhang, Feng Chen, Ying-Zuo Bee & Qing-Mei Xie

  2. Guangdong Wen’s Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu, 527439, China

    Yu Xue, Jian-Ping Qin, Hai-Yan Li & Shuang Chang

Authors
  1. Yu Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing-Lan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhuan-Qiang Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guang-Wei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shun-Yan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiang-Bin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jian-Ping Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hai-Yan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shuang Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Feng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Ying-Zuo Bee
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Qing-Mei Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qing-Mei Xie.

Additional information

Yu Xue, Jing-Lan Wang, and Zhuan-Qiang Yan have contributed equally to this study.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xue, Y., Wang, JL., Yan, ZQ. et al. Sequence and phylogenetic analysis of surface protein genes of emerging H9N2 influenza viruses isolated from poultry in two geographical regions of China. Virus Genes 48, 479–485 (2014). https://doi.org/10.1007/s11262-014-1060-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-014-1060-1

Keywords

Search

Navigation