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A new tospovirus causing chlorotic ringspot on Hippeastrum sp. in China

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Abstract

A new tospovirus, HCRV 2007-ZDH, was isolated from a Hippeastrum sp. plant displaying necrotic and chlorotic ringspot symptoms in Yunnan province. This virus isolate was characterized based on particle morphology and RNA sequences analyses. Quasi-spherical, enveloped particles measuring about 70–100 nm, typical of tospoviruses, were observed in sap and cells of the infected plants. Transmission studies by inoculating this isolate mechanically to Hippeastrum sp. confirmed that 2007-ZDH is the causal agent of the chlorotic ringspot disease of Hippeastrum sp. The complete sequence of S RNA of 2007-ZDH was 2,744 nucleotides in length, sharing 74.4 % nucleotide identity with Tomato yellow ring virus (TYRV) isolate tomato (AY686718). The S RNA encoded a non-structural protein (NSs) (444 aa, 50.4 kDa) and the nucleocapsid (N) protein (273 aa, 30.1 kDa).The deduced NSs protein shared amino acid identities of 78.6, 76.3, and 74.9 % with that of TYRV, IYSV, and PolRSV, respectively. The deduced N protein shared amino acid identities of 86.1, 84.7, and 70.0 % with that of PolRSV, TYRV, and IYSV, respectively. These results suggest that the chlorotic ringspot virus belongs to a new tospovirus species, for which the name Hippeastrum chlorotic ringspot virus (HCRV) is proposed.

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Acknowledgments

This work was supported by Grants from the Major State Basic Research Development Program (2010CB134501), the NSFC (31060237), and the Provincial Innovation Group Foundation of Yunnan (2011CI134).

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Authors and Affiliations

  1. Yunnan Key Laboratory of Agricultural Biotechnology, Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China

    J. H. Dong, Y. Y. Yin, Q. Fang & Z. K. Zhang

  2. Plant Pathology and Biotechnology Laboratory, Agricultural and Forestry Experiment Station, University of Alaska Fairbanks, Fairbanks, AK, 99775, USA

    J. H. Mcbeath

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  1. J. H. Dong
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  2. Y. Y. Yin
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  3. Q. Fang
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  4. J. H. Mcbeath
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  5. Z. K. Zhang
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Correspondence to J. H. Dong or Z. K. Zhang.

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Dong, J.H., Yin, Y.Y., Fang, Q. et al. A new tospovirus causing chlorotic ringspot on Hippeastrum sp. in China. Virus Genes 46, 567–570 (2013). https://doi.org/10.1007/s11262-012-0873-z

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  • DOI: https://doi.org/10.1007/s11262-012-0873-z

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