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Phylogenetic analysis of black queen cell virus genotypes in South Korea

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Abstract

The black queen cell virus (BQCV), a picorna-like honeybee virus, was first isolated from queen larvae and pupae of honeybees found dead in their cells. BQCV is the most common cause of death in queen larvae. Phylogenetic analysis of two Apis cerana and three Apis mellifera BQCV genotypes collected from honeybee colonies in different regions of South Korea, central European BQCV genotypes, and a South African BQCV reference genotype was performed on a partial helicase enzyme coding region (ORF1) and a partial structural polypeptide coding region (ORF2). The phylogeny based on the ORF2 region showed clustering of all the Korean genotypes corresponding to their geographic origin, with the exception of Korean Am str3 which showed more similarity to the central European and the South African reference genotype. However, the ORF1-based tree exhibited a different distribution of the Korean strains, in which A. cerana isolates formed one cluster and all A. mellifera isolates formed a separate cluster. The RT-PCR assay described in this study is a sensitive and reliable method for the detection and classification of BQCV strains from various regions of Korea. BQCV infection is present in both A. cerana and A. mellifera colonies. With this in mind, the present study examined the transmission of honeybee BQCV infections between A. cerana and A. mellifera.

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References

  1. C. Runckel, M.L. Flenniken, J.C. Engel, J.G. Ruby, D. Ganem, R. Andino, J.L. DeRisi, PLoS ONE 6(6), e20656 (2011)

    Article  PubMed  CAS  Google Scholar 

  2. D. van Engelsdorp, J.D. Evans, C. Saegerman, C. Mullin, E. Haubruge, B.K. Nguyen, M. Frazier, J.D. Frazier, Cox-Foster, Y. Chen, R. Underwood, D.R. Tarpy, J.S. Pettis, PLoS ONE 4(8), e6481 (2009)

    Article  Google Scholar 

  3. E. Genersch, M. Aubert, Vet. Res. 41, 54 (2010)

    Article  PubMed  Google Scholar 

  4. L. Bailey, R.D. Woods, J. Gen. Virol. 25, 175–186 (1977)

    Article  Google Scholar 

  5. H.H. Laidlaw Jr., Contemporary Queen Rearing (Dadant & Sons, Hamilton, 1979)

    Google Scholar 

  6. D.L. Anderson, Australas. Beekeep. 94, 292–296 (1993)

    Google Scholar 

  7. G. Topolska, (Warsaw University of Life Sciences, Warsaw, 2008)

  8. D. Tentcheva, L. Gauthier, S. Jouve, L. Canabady-Rochell, B. Dainat, F. Cousserans, M.E. Colin, B.V. Ball, M. Bergoin, Apidologie 35, 431–439 (2004)

    Article  CAS  Google Scholar 

  9. O. Berenyi, T. Bakonyi, I. Derakhshifar, H. Koglberger, N. Nowotny, Appl. Environ. Microbiol. 72, 2414–2420 (2006)

    Article  PubMed  CAS  Google Scholar 

  10. L. Bailey, Bee World 63, 165–173 (1982)

    Google Scholar 

  11. M. Allen, B. Ball, Bee World 77, 141–162 (1996)

    Google Scholar 

  12. N. Leat, B.V. Ball, V. Govan, S. Davison, J. Gen. Virol. 81, 2111–2119 (2000)

    PubMed  CAS  Google Scholar 

  13. D. Tentcheva, L. Gauthier, N. Zappulla, B. Dainat, F. Cousserans, M.E. Colin, M. Bergoin, Appl. Environ. Microbiol. 70, 7185–7191 (2004)

    Article  PubMed  CAS  Google Scholar 

  14. A. Baker, D. Schroeder, J. Invertebr. Pathol. 98, 239–242 (2008)

    Article  PubMed  CAS  Google Scholar 

  15. R. Kajobe, G. Marris, G. Budge, L. Laurenson, G. Cordoni, B. Jones, S. Wilkins, A.G. Cuthbertson, M.A. Brown, J. Invertebr. Pathol. 104(2), 153–156 (2010)

    Article  PubMed  Google Scholar 

  16. X. Yan, J. Chen, R. Han, Sociobiology 53, 687–694 (2009)

    Google Scholar 

  17. M. Mingxiao, L. Ming, C. Jian, Y. Song, W. Shude, L. Pengfei, Funct. Genomics (2011). doi:10.1155/2011/409386

    Google Scholar 

  18. C. Yue, E. Genersch, J. Gen. Virol. 86, 3419–3424 (2005)

    Article  PubMed  CAS  Google Scholar 

  19. Z. Tapaszti, P. Forgach, C. Kovago, G. Topolska, N. Nowotny, M. Rusvai, T. Bakonyi, Vet. Microbiol. 139, 227–234 (2009)

    Article  PubMed  CAS  Google Scholar 

  20. S.E. Choe, L.T. Nguyen, J.H. Noh, H.B. Koh, Y.H. Jean, C.H. Kweon, S.W. Kang, J. Invertebr. Pathol. 109, 330–333 (2012)

    Article  PubMed  Google Scholar 

  21. J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucl. Acids Res. 25, 4876–4882 (1997)

    Article  PubMed  CAS  Google Scholar 

  22. M. Kimura, J. Mol. Evol. 16, 111–120 (1980)

    Article  PubMed  CAS  Google Scholar 

  23. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24(8), 1596–1599 (2007)

    Article  PubMed  CAS  Google Scholar 

  24. N. Saitou, M. Nei, Mol. Biol. Evol. 4, 406–425 (1987)

    PubMed  CAS  Google Scholar 

  25. R. Singh, A.L. Levitt, E.G. Rajotte, E.C. Holmes, N. Ostiguy, D. van Engelsdorp, W.I. Lipkin, C.W. dePamphilis, A.L. Toth, D.L. Cox-Foster, PLoS ONE 5(12), e14357 (2010). doi:10.1371/journal.pone.0014357

    Article  PubMed  CAS  Google Scholar 

  26. J.F.E. Newman, F. Brown, L. Bailey, A.J. Gibbs, J. Gen. Virol. 19, 405–409 (1973)

    Article  CAS  Google Scholar 

  27. O. Berenyi, T. Bakonyi, I. Derakhshifar, H. Koglberger, G. Topolska, W. Ritter, H. Pechhacker, N. Nowotny, Appl. Environ. Microbiol. 73, 3605–3611 (2007)

    Article  PubMed  CAS  Google Scholar 

  28. Y. Kojima, T. Toki, T. Morimoto, M. Yoshiyama, K. Kimura, T. Kadowaki, Microb. Ecol. 62, 895–906 (2011)

    Article  PubMed  Google Scholar 

  29. L. Heath, E.V.D. Walt, A. Varsani, D.P. Martin, J. Virol. 80, 11827–11832 (2006)

    Article  PubMed  CAS  Google Scholar 

  30. A.N. Lukashev, Rev. Med. Virol. 15, 157–167 (2005)

    Article  PubMed  CAS  Google Scholar 

  31. J.L. Li, W.J. Peng, J. Wu, J.P. Strange, B. Boncristiani, Y.P. Chen, J. Econ. Entomol. 104, 732–739 (2011)

    Article  PubMed  Google Scholar 

  32. W.J. Peng, J.L. Li, Apidologie 42, 650–658 (2011)

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the Animal, Plant and Fisheries Quarantine and Inspection Agency, Korean Ministry of Agriculture and Forestry, Republic of Korea.

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Authors and Affiliations

  1. Parasitology and Insect Disease Research Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang, 420-480, South Korea

    Jin Hyeong Noh, Kondreddy Eswar Reddy, Se Eun Choe, Mi Sun Yoo, Huong Thi Thanh Doan, Mummadireddy Ramya, Suk-Chan Jung, Ki-Yoon Chang & Seung Won Kang

  2. Plant Molecular Biology Lab, Department of Botany, S. K. University, Anantapur, Andhrapradesh, India

    Kondreddy Eswar Reddy & Mummadireddy Ramya

  3. Institute of Biotechnology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam

    Huong Thi Thanh Doan, Lien Thi Kim Nguyen, Thuy Thi Dieu Nguyen & Dong Van Quyen

  4. Systemic Disease Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang, 420-480, South Korea

    Chang Hee Kweon

  5. Department of Life Science, College of Natural Science, Kyonggi University, Suwon, 443-760, South Korea

    Byoung-Su Yoon

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  1. Jin Hyeong Noh
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  2. Kondreddy Eswar Reddy
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  3. Se Eun Choe
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  4. Mi Sun Yoo
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  6. Chang Hee Kweon
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  8. Byoung-Su Yoon
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  9. Lien Thi Kim Nguyen
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  10. Thuy Thi Dieu Nguyen
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  11. Dong Van Quyen
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  12. Suk-Chan Jung
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  13. Ki-Yoon Chang
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  14. Seung Won Kang
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Corresponding authors

Correspondence to Kondreddy Eswar Reddy or Seung Won Kang.

Additional information

Jin Hyeong Noh and Kondreddy Eswar Reddy contributed equally to this study.

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Noh, J.H., Reddy, K.E., Choe, S.E. et al. Phylogenetic analysis of black queen cell virus genotypes in South Korea. Virus Genes 46, 362–368 (2013). https://doi.org/10.1007/s11262-012-0859-x

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  • DOI: https://doi.org/10.1007/s11262-012-0859-x

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