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Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

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Abstract

An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95–98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

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Acknowledgments

The work was supported by grants from the University of Bari “Studio di Rotavirus Atipici Umani ed Animali”. J.M. was supported by the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen).

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Author notes

  1. Krisztián Bányai

    Present address: Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary

Authors and Affiliations

  1. Vaccine & Biologics – Clinical Research, Merck Research Laboratories, Mailstop UG3CD-28, P.O. Box 1000, North Wales, PA, 19454-1099, USA

    Max Ciarlet

  2. Department of Microbiology, University of Pennsylvania, Philadelphia, PA, USA

    Christian Hoffmann

  3. Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria di Bari, Bari, Italy

    Eleonora Lorusso, Canio Buonavoglia & Vito Martella

  4. Exopol, Zaragoza, Spain

    Rafael Baselga

  5. Istituto Zooprofilattico di Puglia e Basilicata, Sezione di Foggia, Italy

    Maria Assunta Cafiero

  6. Department of Microbiology & Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium

    Jelle Matthijnssens

  7. Virology Institute, CICV y A, National Institute of Agriculture Technology (INTA), Buenos Aires, Argentina

    Viviana Parreño

  8. Dipartimento di Igiene e Microbiologia, Università di Palermo, Palermo, Italy

    Simona de Grazia

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  1. Max Ciarlet
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Correspondence to Max Ciarlet.

Additional information

Nucleotide sequence accession numbers. Sequence data reported in this manuscript have been deposited in the GenBank Data Library under Accession Numbers EF554151 (VP4), EF554153 (VP7), EF554157 (NSP4), EF554152 (VP6), and EF554158 (NSP5/NSP6) for ovine rotavirus strain OVR762.

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Ciarlet, M., Hoffmann, C., Lorusso, E. et al. Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain. Virus Genes 37, 250–265 (2008). https://doi.org/10.1007/s11262-008-0257-6

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