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Immune responses to oral and IM administration of M2e-Hsp70 construct

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Abstract

The eukaryotic expression plasmid of M2e-Hsp70 is a candidate M2e-based DNA vaccine. In order to evaluate the immunization potential of this construct, Specific Pathogen Free chickens were immunized either intramuscularly or orally. Mutant Salmonella typhimurium was used as carrier for oral delivery of the M2e-Hsp70 construct. M2e-specific humoral and cellular responses were tested by ELISA and Lymphocyte Proliferation Assay, respectively. Our results indicate that both humoral and cellular immune responses are conferred against M2e-Hsp70 plasmid in either of the intramuscular or oral routes of administration; however, these responses are significantly higher in intramuscular injection in contrast to oral administration. When it comes to mass vaccination of commercial chicken flocks oral administration is preferred due to the ease of application as well as its capability of eliciting mucosal, humoral and cellular immune responses; so measurements should be taken to improve the immunization potency of our orally delivered DNA vaccine.

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Acknowledgments

This project was financially supported by grant no. 7502015/6/16 supplied by University of Tehran.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Farzaneh Assadian, Gholamreza Nikbakht & Monireh Jahantigh

  2. Central veterinary laboratory, Iran veterinary organization, Tehran, Iran

    Siavash Niazi & Reza Farahani Khaltabadi

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  1. Farzaneh Assadian
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  2. Gholamreza Nikbakht
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  3. Siavash Niazi
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  4. Reza Farahani Khaltabadi
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Correspondence to Farzaneh Assadian.

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Assadian, F., Nikbakht, G., Niazi, S. et al. Immune responses to oral and IM administration of M2e-Hsp70 construct. Vet Res Commun 38, 157–163 (2014). https://doi.org/10.1007/s11259-014-9599-9

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