Veterinary Research Communications

, Volume 37, Issue 3, pp 217–228

Efficacy of anti-staphylococcal protein P128 for the treatment of canine pyoderma: potential applications

  • Raghu Patil Junjappa
  • Srividya Narayanamurthy Desai
  • Panchali Roy
  • Nagalakshmi Narasimhaswamy
  • Juliet Roshini Mohan Raj
  • Murali Durgaiah
  • Aradhana Vipra
  • Udaya Ravi Bhat
  • Smitha Komarla Satyanarayana
  • Nandini Shankara
  • SuneelKumar Muragesh Basingi
  • Jagadeesh Janardhan Bhat
  • Sukumar Hariharan
  • Bharathi Sriram
  • Sriram Padmanabhan
Original Article

DOI: 10.1007/s11259-013-9565-y

Cite this article as:
Junjappa, R.P., Desai, S.N., Roy, P. et al. Vet Res Commun (2013) 37: 217. doi:10.1007/s11259-013-9565-y

Abstract

In this study, we demonstrate the antibacterial activity of P128 on Staphylococcus isolates responsible for canine pyoderma. Eighty seven swabs were collected from dogs suffering from pyoderma and subjected to antibiotic sensitivity test and 46 Staphylococcus strains were isolated and characterized. In-vitro antimicrobial susceptibility testing with P128 was done by Minimum Inhibitory Concentration (MIC) method as per CLSI guidelines. All the Staphylococci isolated from the dogs with pyoderma, although showed resistance to various antibiotics tested, were lysed by P128. Clinical efficacy of P128 was examined in 17 dogs with pyoderma by application of the P128 hydrogel twice daily for 8 days and the results indicated complete healing of all the lesions of all the dogs under treatment. Under the conditions of this study, P128 was found to be a potent convenient proteinaceous drug for the treatment of staphylococcal pyoderma in dogs.

Keywords

Antibiotic resistant Staphylococci Canine pyoderma Coagulase positive staphylococci Coagulase negative staphylococci P128 hydrogel Staphylococcus intermedius Staphylococcus pseudintermedius 

Abbreviations

CFU

Colony forming units

CLSI

Clinical and Laboratory Standards Institute

CoNS

Coagulase negative staphylococci

CoPS

Coagulase positive staphylococci

CPD

Canine pyoderma

GRAS

Generally regarded as safe

LB

Luria Bertani

MIC

Minimum inhibitory concentration

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Raghu Patil Junjappa
    • 1
  • Srividya Narayanamurthy Desai
    • 1
  • Panchali Roy
    • 1
  • Nagalakshmi Narasimhaswamy
    • 2
  • Juliet Roshini Mohan Raj
    • 1
  • Murali Durgaiah
    • 1
  • Aradhana Vipra
    • 1
  • Udaya Ravi Bhat
    • 3
  • Smitha Komarla Satyanarayana
    • 1
  • Nandini Shankara
    • 1
  • SuneelKumar Muragesh Basingi
    • 1
  • Jagadeesh Janardhan Bhat
    • 1
  • Sukumar Hariharan
    • 1
  • Bharathi Sriram
    • 1
  • Sriram Padmanabhan
    • 1
  1. 1.Gangagen Biotechnologies Pvt. Ltd.BangaloreIndia
  2. 2.Department of MicrobiologyMelaka Manipal Medical CollegeManipalIndia
  3. 3.Prajna Veterinary ClinicBangaloreIndia