Serum protein pattern in ewe with pregnancy toxemia
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Pregnancy toxemia is a metabolic disease of pregnant ewes which causes significant economic losses in sheep industry. The pathophysiology and metabolic changes of this disorder remain poorly understood. We conducted this study to describe the serum protein pattern associated with the pregnancy toxemia in ewes. In this study, the electrophoretic pattern of serum proteins of 15 ewes with naturally occuring pregnancy toxemia and 12 ewes with uncomplicated pregnant were investigated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Serum protein patterns were mainly characterized by four bands and located in the 76 kDa, 66 kDa, 55 kDa and 29 kDa both diseased and control groups. The percent of the 66 kDa, 55 kDa and 29 kDa proteins were decreased (P < 0.001 for 66 kDa; P < 0.01 for 55 kDa and P < 0.05 for 29 kDa) while 76 kDa (P < 0.05) protein was significantly increased (P < 0.001) in ewes with pregnancy toxemia relative to controls. Positive correlations were found between activities of liver enzymes and percentage of the distribution in 76 kDa, 55 kDa proteins. In contrast, there was a negative correlation between the 66 kDa protein and liver enzymes. In conclusion, the results of this study demonstrate that the percentages of the 76 kDa, 66 kDa, 55 kDa and 29 kDa proteins are significantly altered in ewes with pregnancy toxemia. However, further studies are needed to explore the potential role of these alterations in the pathophysiology in ewe with pregnancy toxemia.
- Altintas, A., Sahal, M., Celik, S., Duru, O. and Ocal, N., 2001, Serum ve idrar proteinlerinin elektroforetik analizi ve veteriner hekimlikteki onemi. Turk J Vet Anim Sci 25, 93–104.
- Andrews, A., 1997, Pregnancy toxemia in the ewe. In Practice 19, 306–312.
- Andrews, A.H., Holland-Hoewes, V.E. and Wilkinson, J.I.D., 1996, Naturally occurring pregnancy toxaemia in the ewe and treatment with recombinant bovine somatotropin. Small Rum Res 23, 191–197. doi:10.1016/S0921-4488(96)00912-1 CrossRef
- Camacho, A.T., Guitian, F.J., Pallas, E., Gestal, J.J., Olmeda, S., Goethert, H., Telford, S. and Spielman, A., 2005, Serum protein response and renal failure in canine Babesia annae infection. Vet Res 36, 713–722. doi:10.1051/vetres:2005026 CrossRef
- Cantley, C.E., Ford, C.M. and Heath, M.F., 1991, Serum fructosamine in ovine pregnancy toxaemia: a possible prognostic index. Vet Rec 128, 525–526.
- David, F., 2003, Protein electrophoresis in clinical diagnosis. Oxford University Press, New York.
- Giordano, A., Spagnolo, V., Colombo, A. and Paltrinieri, S., 2004, Changes in some acute phase protein and immunoglobulin concentrations in cats affected by feline infectious peritonitis or exposed to feline coronavirus infection. Vet J 167, 38–44. doi:10.1016/S1090-0233(03)00055-8 CrossRef
- Gojnic, M., Petkovic, S., Papic, M., Mostic, T., Jeremic, K., Vilendecic, Z. and Djordjevic, S., 2004, Plasma albumin level as an indicator of severity of preeclampsia. Clin Exp Obstet Gynecol 31, 209–210.
- Harmeyer, J. and Schlumbohm, C., 2006, Pregnancy impairs ketone body disposal in late gestating ewes: Implications for onset of pregnancy toxaemia. Res Vet Sci 81, 254–264. doi:10.1016/j.rvsc.2005.10.010 CrossRef
- Harrus, S., Waner, T., Avidar, Y., Bogin, E., Peh, H. and Bark, H., 1996, Serum protein alterations in canine ehrlichiosis. Vet Parasitol 66, 241–249. doi:10.1016/S0304-4017(96)01013-8 CrossRef
- Hay, L.A. and Baird, G.D., 1991, Pregnancy toxaemia. In: Martin, W. B. and I. D. Aitken (ed.), Diseases of Sheep, pp: 254–257. Blackwell Scientific Publications, Oxford.
- Henze, P., Bickhardt, K. and Fuhrmann, H., 1994, The influences of insulin, cortisol, growth hormone and total oestrogen on the pathogenesis of ketosis in sheep. Dtsch Tierärztl Wschr 101, 61–65.
- Honger, P.E., 1966, Albumin metabolism in preeclampsia. Acta Obstet Gynecol Scand 45, 104–105. CrossRef
- Jeffrey, M. and Higgins, R.J., 1992, Brain lesions of naturally occurring pregnancy toxemia of sheep. Vet Pathol 29, 301–307. CrossRef
- Kaneko, J.J., Harvey, J.W. and Bruss, M.L., 1997, Clinical Biochemistry of Domestic Animals, 5th edn. Academic Press Inc., California, U.S.A.
- Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685. doi:10.1038/227680a0 CrossRef
- Marteniuk, J.V. and Herdt, T.H., 1988, Pregnancy toxemia and ketosis of ewes and does. Vet Clin North Am Food Anim Pract 4, 307–315.
- Rao, C.R., 1973, Linear statistical inference and its applications. John&Sons, New York. CrossRef
- Ritzman, S.E. and Daniels, J.C., 1982, Serum protein electrophoresis and total serum proteins. In: Ritzman S. E., and J. C. Daniels (ed.), Serum Protein Abnormalities: Diagnostic and Clinical Aspects. Alan R. Liss Inc., New York.
- Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989, Molecular Cloning, a Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Press, New York.
- Sargison, N.D., Scott, P.R., Penny, C.D., Pirie, R.S. and Kelly, J.M., 1994, Plasma enzymes and metabolites as potential prognostic indices of ovine pregnancy toxaemia–a preliminary study. Br Vet J 150, 271–277.
- Scott, P.R., Sargison, N.D., Penny, C.D., Pirie, R.S. and Kelly, J.M., 1995, Cerebrospinal fluid and plasma glucose concentrations of ovine pregnancy toxaemia cases, inappetant ewes and normal ewes during late gestation. Br Vet J 151, 39–44. CrossRef
- Thomson, G.G., 1956, Observations on some treatements of ovine pregnancy toxemia. N Z Vet J 4, 136–144.
- Tontis, A. and Zwahlen, R., 1987, Pregnancy toxemia of small ruminants with special reference to pathomorphology. Tierarztl Prax 15, 25–29.
- Serum protein pattern in ewe with pregnancy toxemia
Veterinary Research Communications
Volume 33, Issue 5 , pp 431-438
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