Abstract
Purpose
The aim of this study was to investigate the number of autophagosomes in podocyte from kidney tissue of immunoglobulin A nephropathy (IgAN) and idiopathic membranous nephropathy (IMN).
Methods
The changes in kidney tissue pathology were detected after hematoxylin and eosin, periodic acid-Schiff, Masson’s trichrome and immunofluorescence. The autophagosomes of podocyte were analyzed by transmission electron microscopy. Clinical data, including age, gender, edema, serum creatinine, estimated glomerular filtrating rate (eGFR), hematuria, urine protein excretion and serum albumin, were collected from inpatient medical record.
Results
It was found that the number of autophagosomes in podocyte of nephropathy group was lower when compared to the control group. At the same time, we did not find the difference of the parameter between these two kinds of nephropathy. Further study showed that the index was affected by two factors: eGFR and gender. The cases with worse eGFR (eGFR < 60 ml/min) and male patients presented more autophagosomes. Furthermore, each nephropathy had its own character. The phenomena of reduced autophagosomes were found in IMN cases, did not change from male to female, and further aggravated from pathological stage I to II. By contrast, IgAN cases with less eGFR exhibited more autophagosomes.
Conclusions
Therefore, the results of the present study indicate that autophagy participates in podocyte injury and the progression of IgAN and IMN.
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Acknowledgments
This study was funded by the General Project of the Medical and Health of Zhejiang Province (Grant Number 2014kYA014), the Project of Scientific research Foundation of Chinese Medicine (Grant Number 2014ZB002), the Project of Clinical and Scientific research Foundation of Medical associate of Zhejiang Province (Grant Number 2013ZYC-A01), the General Project of the Medical and Health of Zhejiang Province (Grant Number 2015KYA025), the Project of Scientific research Foundation of Chinese Medicine (Grant Number 2015ZA011) and Zhejiang Provincial Program for the Cultivation of High-level New Health Talent (Grant Number 2013).
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
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Informed consent was obtained from all individual participants included in the study.
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Liang, S., Jin, J., Gong, J. et al. How many podocyte autophagosomes are there in immunoglobulin A nephropathy and idiopathic membranous nephropathy?. Int Urol Nephrol 48, 2109–2114 (2016). https://doi.org/10.1007/s11255-016-1398-5
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DOI: https://doi.org/10.1007/s11255-016-1398-5