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Histopathological classification criteria of rat model of chronic prostatitis/chronic pelvic pain syndrome

  • Urology - Original Paper
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Abstract

Objective

A variety of murine models of experimental prostatitis that mimic the phenotype of human chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) have been developed. However, there is still a lack of explicit diagnosis criteria about those animal model. Our study is to establish histopathological classification criteria, which will be conducive to evaluate the animal models.

Methods

We firstly established a rat model of experimental autoimmune prostatitis that is considered a valid model for CP/CPPS. For modelling, male Sprague–Dawley rats were immunized with autologous prostate tissue homogenate supernatant emulsified with complete Freund’s adjuvant by subcutaneous injection into abdominal flank and simultaneously immunized with pertussis–diphtheria–tetanus vaccine by intraperitoneal injection. Three immunizations were administered semimonthly. At the 45th day, animals were killed, and prostate tissues were examined for morphology.

Results

Histologically, the prostate tissues were characterized by lymphoproliferation, atrophy of acini, and chronic inflammatory cells infiltration in the stromal connective tissue around the acini or ducts. Finally, we built histopathological classification criteria incorporating inflammation locations (mesenchyme, glands, periglandular tissues), ranges (focal, multifocal, diffuse), and grades (grade I–IV). To verify the effectiveness and practicability of the histopathological classification criteria, we conducted the treatment study with one of the alpha blockers, tamsulosin.

Conclusion

The histopathological classification criteria of rat model of CP/CPPS will serve for further research of the pathogenesis and treatment strategies of the disease.

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Acknowledgments

This study was supported by the grants from the National Natural Science Foundation of China (81270195), and the Key Project of Science and Technology Commission of Shanghai (SHD09411950400). We thank Shanghai Showbio, Biotech Co., Ltd. and Dr Yan-Qin Hu (Shanghai Key Laboratory of Reproductive Medicine, Shanghai, China) for their expert technical assistance.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Urology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197# Ruijin Er Road, Shanghai, 200025, People’s Republic of China

    Xianjin Wang, Shan Zhong, Tianyuan Xu, Leilei Xia, Xiaohua Zhang, Zhaowei Zhu, Minguang Zhang & Zhoujun Shen

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  1. Xianjin Wang
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  2. Shan Zhong
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Correspondence to Zhoujun Shen.

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Xianjin Wang and Shan Zhong have contributed equally to this work.

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Wang, X., Zhong, S., Xu, T. et al. Histopathological classification criteria of rat model of chronic prostatitis/chronic pelvic pain syndrome. Int Urol Nephrol 47, 307–316 (2015). https://doi.org/10.1007/s11255-014-0868-x

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