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Urinary chemokines and anti-inflammatory molecules in renal transplanted patients as potential biomarkers of graft function: a prospective study

  • Nephrology – Original Paper
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Abstract

Purpose

Clinical- and histopathology-based scores are the limited predictors of allograft outcome. Thus, predictors of allograft survival still remain a challenge. This study aimed to evaluate the urinary levels of chemokines and anti-inflammatory molecules at 30, 90, and 300 days after renal transplantation and to further correlate these measurements to graft function.

Methods

Glomerular filtration rate (GFR) and urinary levels of MCP-1/CCL2, MIP-1α/CCL3, RANTES/CCL5, IL-8/CXCL8, IP-10/CXCL10, interleukin-1 receptor antagonist, soluble tumor necrosis factor receptor-1, and receptor-2 were determined at 30, 90, and 300 days after renal transplantation in 22 patients. Transplanted patients were also divided according to the type of donor (living donor, LD, n = 13 or deceased donor, DD, n = 9).

Results

Urinary levels of all molecules, except MIP-1α/CCL3, remained unchanged at 30, 90, and 300 days after transplantation in our 22 patients. MIP-1α/CCL3 levels significantly reduced from 30 to 300 days and showed a negative correlation with GFR at 30 days. The comparison between LD and DD groups showed similar levels of all markers, except for MCP-1/CCL2, which presented higher values in LD than in DD at 30 days. sTNFR1 and MCP-1/CCL2 significantly reduced from 30 to 300 days in LD group, but only sTNFR2 concentrations at 30 days were negatively correlated with GFR at 300 days. On the other hand, in DD group, IL-1Ra concentrations at 30 and at 90 days were positively correlated with GFR at 300 days.

Conclusion

Urinary chemokine and anti-inflammatory molecules measurements may be a promising tool in the follow-up of renal transplanted patients.

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Acknowledgments

This study was partially supported by CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil) and FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil) by the Grant INCT-MM (Instituto Nacional de Ciência e Tecnologia—Medicina Molecular: FAPEMIG: CBB-APQ-00075-09/CNPq 573646/2008-2). Dr. AL Teixeira, Dr. EA Oliveira, Dr. MM Teixeira, and Dr. AC Simões e Silva received a research productivity fellowship from the CNPq.

Conflict of interest

None declared.

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Authors and Affiliations

  1. Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, 30130-100, Brazil

    André Barreto Pereira, Antônio Lúcio Teixeira & Nilton Alves Rezende

  2. Fundação Hospitalar do Estado de Minas Gerais, Belo Horizonte, MG, Brazil

    Regina Maria Pereira

  3. Departamento de Pediatria, Faculdade de Medicina, Instituto Nacional de Ciência e Tecnologia (INCT) de Medicina Molecular, Faculdade de Medicina—UFMG, Avenida Alfredo Balena, 190, Belo Horizonte, MG, 30130-100, Brazil

    Débora Marques Miranda, Eduardo Araujo Oliveira & Ana Cristina Simões e Silva

  4. Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas—UFMG, Belo Horizonte, MG, 31270-901, Brazil

    Mauro M. Teixeira

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  1. André Barreto Pereira
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Correspondence to Ana Cristina Simões e Silva.

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Pereira, A.B., Teixeira, A.L., Rezende, N.A. et al. Urinary chemokines and anti-inflammatory molecules in renal transplanted patients as potential biomarkers of graft function: a prospective study. Int Urol Nephrol 44, 1539–1548 (2012). https://doi.org/10.1007/s11255-012-0176-2

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  • DOI: https://doi.org/10.1007/s11255-012-0176-2

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