Abstract
Alpha-lactalbumin has been reported as a highly polymorphic gene that potentially alters the gene expression and is associated with milk composition in dairy breeds. Current study was conducted in two phases. In the first phase, polymorphisms identification in alpha-lactalbumin (LALBA) gene and its association with milk composition was performed. To identify the genetic polymorphism, Nili Ravi buffaloes at their second lactation were selected from Government livestock farm (Buffalo Research Institute, Pattoki). Genomic DNA was extracted from blood samples. After PCR amplification, products were sequenced, and data was analyzed. Results showed that the identified polymorphisms at chromosomal position 34310940 were found associated with major whey protein. In the second phase of study, milk samples were collected from five healthy mastitis-free Nili Ravi buffaloes in their second lactation for expression analysis of alpha-lactalbumin gene at their transition (day 15), mid (day 90), and late (day 250) lactation. Gene expression was observed highest in transition phase with a gradual decrease of expression in mid and late phase of lactation. Further studies are needed to explore the regulation of milk production genes and their translational efficiency during the course of lactation.
Similar content being viewed by others
References
Bionaz, M. and J. J.Loor. 2007. Identification of reference genes for quantitative real-time PCR in the bovine mammary gland during the lactation cycle. Physiol. Genomics. 29: 312–319.
Bojarojc-Nosowicz, B., E. Kaczmarczyk, E. M. I. L. I. A. Bongarc, and J. Malolepszy. 2005. Natural BLV infection and polymorphism within the 5′ flanking region of α-lactalbumin gene in black-and-white breed cattle. Bull. Vet. 49: 439–442.
Chacon-Cortes, D., and L. R. Griffiths. 2014. Methods for extracting genomic DNA from whole blood samples: current perspectives. J. Biorepos. Sci. Appl. Med. 2014(2): 1–9.
Cross, M.L. and H. S. Gill. 2000. Immunomodulatory properties of milk. Brit J Nutr. 84: 81–89.
Dayal, S., T. K. Bhattacharya, V. Vohra, P. Kumar, and A. Sharma. 2006. Effect of Alpha-lactalbumin Gene Polymorphism on Milk Production Traits in Water Buffalo. Asian-Aust. J. Anim. Sci. 19(3): 305–308.
Friggens, N.C., C. Ridder, and P. Løvendahl. 2007. On the use of milk composition measures to predict the energy balance of dairy cows. J. Dairy Sci. 90(12): 5453–67.
Gao, Y., X. Lin, K. Shi, Z. Yan, and Z. Wang. 2013. Bovine mammary gene expression profiling during the onset of lactation. PLoS One. 8(8): e70393.
Gáspárdy, A., Z. Schwartz, L. Zöldág, T. Veresegyházy, and S. Fekete. 2004. Changes in daily energy amounts of main milk components (lactose, protein and fat) during the lactation of high-yielding dairy cows. Acta. Veterinaria Hungarica. 52: 457–467.
Gautier, M., L. Flori, A. Riebler, F. Jaffrézic, D. Laloé, I. Gut, K. Moazami-Goudarzi, and J. L. Foulley. 2009. A whole genome Bayesian scan for adaptive genetic divergence in West African cattle. BMC Genomics. 10: 550.
Kadegowda, A.K.G., M. Bionaz, B. Thering, L. S. Piperova, R. A. Erdmanand J. J. Loor. 2009. Identification of internal control genes for quantitative polymerase chain reaction in mammary tissue of lactating cows receiving lipid supplements. J. Dairy Sci. 92(5): 2007–2019.
Lemay, D.G., D. J. Lynn, W. F. Martin, M. C. Neville, T. M. Casey, G. Rincon, E. V. Kriventseva, W. C. Barris, A. S. Hinrichs, A. J. Molenaar, and K. S. Pollard. 2009. The bovine lactation genome: insights into the evolution of mammalian milk. Genome Biol. 10: 43–54.
Littlejohn, M.D., C. G. Walker, H. E. Ward, K. B. Lehnert, R. G. Snell, G. A. Verkerk, R. J. Spelman, D. A. Clark, and S. R. Davis. 2010. Effects of reduced frequency of milk removal on gene expression in the bovine mammary gland. Physiol. Genomics. 41(1): 21–32.
Madureira, A.R., C. I. Pereira, A. M. Gomes, M. E. Pintado, and F. X. Malcata. 2007. Bovine whey proteins–Overview on their main biological properties. Food Res. Int. 40(10): 1197–1211.
Markus, C.R., B. Olivier, and E. H. de Haan. 2002. Whey protein rich in alpha-lactalbumin increases the ratio of plasma tryptophan to the sum of the other large neutral amino acids and improves cognitive performance in stress-vulnerable subjects. Am. J. Clin. Nutr. 75(6): 1051–1056.
Martins, L.F., M. P. Milazzotto, W. B. Feitosa, A. R. S. Coutinho, R. Sim, M. G. Marques, M. E. O. A. Assumpcao, and J. A. Visintin. 2008. Sequence Variation Of the α-Lactalbumin Gene In Holstein and Nellore Cows. Anim. Biotechnol. 19(3): 194–198.
Matsumoto, H., Y. Shimokawa, Y. Ushida, T. Toida, and H. Hayasawa. 2001. New biological function of bovine α-lactalbumin: Protective effect against ethanol- and stress-induced gastric mucosal injury in rats. Biosci. Biotech. Biochem. 65: 1104–1111.
Mir, S.N., O. Ullah, and R. Sheikh. 2014. Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle. Afr. J. Biotechnol. 13(4).
Mura, M. C., C. Daga, S. Bodano, M. Paludo, S. Luridiana, M. Pazzola, and V. Carcangiu. 2013. Development of a RNA extraction method from milk for gene expression study in the mammary gland of sheep. Mol. Biol. Rep. 40: 2169–2173.
Nicolazzi, E.L., M. Picciolini, F. Strozzi, R. D. Schnabel, C. Lawley, A. Pirani, F. Brew, and A. Stella. 2014. SNPchiMp: a database to disentangle the SNPchip jungle in bovine livestock. BMC Genomics. 15: 123.
Pellegrini, A. 2003. Antimicrobial peptides from food proteins. Curr Pharm Des. 9(16): 1225–1238.
Petrotos, K., E. Tsakali, P. Goulas, and A. G. D’Alessandro. 2014 Casein and Whey Proteins in Human Health. In Kanekanian A editor. Milk and Dairy Products as Functional Foods, 1st ed. Publishing John Wiley & Sons, Ltd. Chichester.
Piantoni, P., P. Wang, J. K. Drackley, W. L. Hurley, and J. J. Loor. 2010. Expression of metabolic, tissue remodeling, oxidative stress, and inflammatory pathways in mammary tissue during involution in lactating dairy cows. Bioinform. Biol. Insights. 4: 85–97.
Quist, M.A., S. J. LeBlanc, K. J. Hand, D. Lazenby, F. Miglior, and D. F. Kelton. 2008. Milking-to-milking variability for milk yield, fat and protein percentage, and somatic cell count. J. Dairy Sci. 91(9): 3412–3423.
Ramesha, K.P., H. Khosravinia, S. Gowda, and M. R. S. Rao. 2008. Alpha-Lactalbumin Gene Polymorphism: A preliminary study on two breeds of the river Buffalo (Bubalus bubalis). AsPac. J. Mol. Biol. Biotechnol. 16 (2): 47–52.
Sigl, T., H. H. D. Meyer, and S. Wiedemann. 2012. Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation. Czech J. Anim. Sci. 57(10): 469–480.
Sternhagen, L.G. and J. C. Allen. 2001. Growth rates of a human colon adenocarcinoma cell line are regulated by the milk protein alpha-lactalbumin. Adv. Exp. Med. Biol. 501: 115–120.
Strucken, E.M., Y. C. Laurenson, and G. A. Brockmann. 2015. Go with the flow-biology and genetics of the lactation cycle. Front. Genet. 6: 118.
Svensson, M., A. Håkansson, A. K. Mossberg, S. Linse, and C. Svanborg. 2000. Conversion of alpha-lactalbumin to a protein inducing apoptosis. Proc. Natl. Acad. Sci. U S A. 97(8): 4221–4226.
VanRaden, P.M., K. M. Olson, D. J. Null, M. Sargolzaei, M. Winters, and J. B. Van Kaam. 2012. Reliability increases from combining 50, 000 and 777, 000-marker genotypes from four countries. Interbull Bull. 46: 75–79.
Wickramasinghe, S., G. Rincon, A. Islas-Trejo, and J. F. Medrano. 2012. Transcriptional profiling of bovine milk using RNA sequencing. BMC Genomics.13: 45–56.
Yong-qiang, Y., G. Yu, J. Ren-gang, H. Yan-ting, and L. Ruo-yu. 2013. Transcriptional profiling of bovine milk using RNA sequencing. BMC Genomics.13: 45–56.
Yudin, N. S. and M. I. Voevoda. 2015. Molecular Genetic Markers of Economically Important Traits in Dairy Cattle. Genetika. 51(5): 600–612.
Zhang, J., D. Sun, J. E. Womack, Y. Zhang, Y. Wang, and Y. Zhang. 2007. Polymorphism Identification, RH Mapping and Association of α-Lactalbumin Gene with Milk Performance Traits in Chinese Holstein. Asian-Aust. J. Anim. Sci. 20(9): 1327–1333.
Zhou, J.P. and C. H. Dong, 2013. Association between a polymorphism of the α-lactalbumin gene and milk production traits in Chinese Holstein cows. Genet. Mol. Res. 12(3): 3375–3382.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Manzoor, S., Nadeem, A. & Javed, M. Polymorphism association and expression analysis of alpha-lactalbumin (LALBA) gene during lactation in Nili Ravi buffalo. Trop Anim Health Prod 52, 265–271 (2020). https://doi.org/10.1007/s11250-019-02010-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11250-019-02010-0