Skip to main content
SpringerLink
Log in

Osteopontin gene polymorphism association with milk traits and its expression analysis in milk of riverine buffalo

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Osteopontin gene is regarded as a plausible candidate in mammary gland differentiation and development, expressed by variety of cells, tissues, and biological fluids including milk. The current study was performed in two phases. In the first phase, Osteopontin gene polymorphisms were identified and associated with milk composition such as ash, milk fat, SNF, lactose, and protein. In the second phase, milk samples from five healthy mastitis-free Nili Ravi buffaloes were analyzed for expression of Osteopontin gene at transition (day 15), mid (day 90), and end (day 250) stage of their second lactation. Briefly, blood samples were collected from Nili Ravi buffalo to isolate the genomic DNA, specific primers were designed for PCR amplification. The amplified PCR products were sequenced bi-directionally. Six polymorphisms were identified in the coding region and four in the intronic region of the gene. The results showed that SNP g.38329758 T > C causing substitution of valine to alanine (V127A) was associated with high milk protein. For mRNA expression analysis, somatic cells were separated from milk samples for RNA isolation. Analysis of differential gene expression data has permitted us to illustrate the expression pattern of osteopontin gene in lactating buffalo. The Osteopontin gene was found to be transcribed among all three lactation stages, but expression was observed with the highest value (fold change) in peak lactation and remained elevated till the end of lactation. Identified gene marker may be helpful for the prediction of superior animal for selection. The presented study also gave an insight into the genetic screening and lactation biology of riverine buffalo, offering direction for future research in lactating buffalo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1

Similar content being viewed by others

References

  • Anborgh, P. H., Mutrie, J. C., Tuck, A. B. and Chambers, A. F., 2011. Pre-and post-translational regulation of osteopontin in cancer, Journal of Cell Communication and Signaling, 5, 111–122

    Article  PubMed  PubMed Central  Google Scholar 

  • Bustin, S.A., Benes, V., Garson, J.A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M.W., Shipley, G.L. and Vandesompele, J., 2009. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 55, 611–622

    Article  CAS  PubMed  Google Scholar 

  • Chatterton, D. E. W., Rasmussen, J. T., Heegaard, C. W., Sørensen, E. S. and Petersen, T. E., 2004. In vitro digestion of novel milk protein ingredients for use in infant formulas: research on biological functions, Trends in Food Science and Technology, 15, 373–383

    Article  CAS  Google Scholar 

  • Christensen, B. and Sorensen, E. S., 2016. Structure, function and nutritional potential of milk osteopontin, International Dairy Journal, 57, 1–6

    Article  CAS  Google Scholar 

  • Cohen, M., Seroussi, E., Band, M. R., Lewin, H. A., Drackley, J. K., Larkin, D. M. and Weller, J. I., 2004. SPP1 is a candidate gene for the QTL affecting milk protein concentration on BTA6 in Israeli Holstein, In ISAG, Proceedings of the 29th International Conference of Animal Genetics, F015, Tokyo, Japan

  • Cohen-Zinder, M., Seroussi, E., Larkin, D. M., Loor, J. J., Everts-van der Wind, A., Lee, J. H. and Lewin, H. A., 2005. Identification of a missense mutation in the bovine ABCG2 gene with a major effect on the QTL on chromosome 6 affecting milk yield and composition in Holstein cattle, Genome Research, 15, 936–944

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jatav, P., Sodhi, M., Sharma, A., Mann, S., Kishore, A., Shandilya, U. K and Kumar, S., 2016. Identification of internal control genes in milk-derived mammary epithelial cells during lactation cycle of Indian zebu cow, Animal Science Journal, 87, 344–353

    Article  CAS  PubMed  Google Scholar 

  • Khatkar, M. S., Thomson, P. C., Tammen, I. and Raadsma, H. W., 2004. Quantitative trait loci mapping in dairy cattle: review and meta-analysis, Genetics Selection Evolution, 36, 1

    Article  Google Scholar 

  • Leonard, S., Khatib, H., Schutzkus, V., Chang, Y. M. and Maltecca, C., 2005. Effects of the osteopontin gene variants on milk production traits in dairy cattle, Journal of Dairy Science, 88, 4083–4086

    Article  CAS  PubMed  Google Scholar 

  • Khatib, H., Zaitoun, I., Wiebelhaus-Finger, J., Chang, Y. M. and Rosa, G. J. M., 2007. The association of bovine PPARGC1A and OPN genes with milk composition in two independent Holstein cattle populations, Journal of Dairy Science, 90, 2966–2970

    Article  CAS  PubMed  Google Scholar 

  • Livak, K. J. and Schmittgen, T. D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2—ΔΔCT method, Methods, 25, 402–408

    Article  CAS  PubMed  Google Scholar 

  • Mazzali, M., Kipari, T., Ophascharoensuk, V., Wesson, J. A., Johnson, R. and Hughes, J., 2002. Osteopontin: a molecule for all seasons, QJM, 95, 3–13

    Article  CAS  PubMed  Google Scholar 

  • Mello, F., Cobuci, J. A., Martins, M. F., Silva, M. V. G. B. and Neto, J. B., 2012. Association of the polymorphism g. 8514C> T in the osteopontin gene (SPP1) with milk yield in the dairy cattle breed Girolando, Animal Genetics, 43, 647–648

    Article  PubMed  Google Scholar 

  • Mura, M. C., Daga, C., Bodano, S., Paludo, M., Luridiana, S., Pazzola, M. and Carcangiu, V., 2013. Development of a RNA extraction method from milk for gene expression study in the mammary gland of sheep, Molecular Biology Reports, 40, 2169–2173

    Article  CAS  PubMed  Google Scholar 

  • Nagatomo, T., Ohga, S., Takada, H., Nomura, A., Hikino, S., Imura, M. and Hara, T., 2004. Microarray analysis of human milk cells: persistent high expression of osteopontin during the lactation period, Clinical and Experimental Immunology, 138, 47–53

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nemir, M., Bhattacharyya, D., Li, X., Singh, K., Mukherjee, A. B. and Mukherjee, B. B., 2000. Targeted inhibition of osteopontin expression in the mammary gland causes abnormal morphogenesis and lactation deficiency, Journal of Biological Chemistry, 275, 969–976

    Article  CAS  PubMed  Google Scholar 

  • Pareek, C. S., Czarnik, U., Pierzchała, M. and Zwierzchowski, L., 2008. An association between the C> T single nucleotide polymorphism within intron IV of osteopontin encoding gene (SPP1) and body weight of growing Polish Holstein-Friesian cattle, Animal Science Papers and Reports, 26, 251–257

    CAS  Google Scholar 

  • Pasandideh, M., Mohammadabadi, M. R., Esmailizadeh, A. K. and Tarang, A., 2015. Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle, Czech Journal of Animal Science, 60, 97–104

    Article  CAS  Google Scholar 

  • Piantoni, P., Wang, P., Drackley, J. K., Hurley, W. L. and Loor, J. J., 2010. Expression of metabolic, tissue remodeling, oxidative stress, and inflammatory pathways in mammary tissue during involution in lactating dairy cows, Bioinformatics and Biology Insights, 4, 85

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Salehi, A., Aminafshar, M., Sobhani, R. and Sayaadnejad, M. B., 2015. The Association of Bovine Osteopontin (OPN) Gene with Milk Production Traits in Iranian Holstein Bulls, Iranian Journal of Biotechnology, 13, 43–48

    Article  PubMed  PubMed Central  Google Scholar 

  • Sambrook J, Russell DW. 2001. Molecular cloning: a laboratory manual 3rd edition, (Coldspring-Harbour Laboratory Press, UK)

    Google Scholar 

  • Schack, L., Lange, A., Kelsen, J., Agnholt, J., Christensen, B., Petersen, T. E. and Sørensen, E. S., 2009. Considerable variation in the concentration of osteopontin in human milk, bovine milk, and infant formulas, Journal of Dairy Science, 92, 5378–5385

    Article  CAS  PubMed  Google Scholar 

  • Schnabel, R. D., Kim, J. J., Ashwell, M. S., Sonstegard, T. S., Van Tassell, C. P., Connor, E. E. and Taylor, J. F., 2005. Fine-mapping milk production quantitative trait loci on BTA6: analysis of the bovine osteopontin gene. Proceedings of the National Academy of Sciences of the United States of America, 102, 6896–6901

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sodek, J., Ganss, B. and McKee, M. D., 2000. Osteopontin, Critical Reviews in Oral Biology and Medicine, 11, 279–303

    Article  CAS  PubMed  Google Scholar 

  • Tantia, M. S., Mishra, B., Bharani Kumar, S. T., Mishra, B. P., Kataria, R. S., Mukesh, M. and Vijh, R. K., 2008. Characterization of osteopontin gene of Bubalus bubalis, (Animal-Cambridge University Press)

  • Weber, G. F., Lett, G. S. and Haubein, N. C., 2011. Categorical meta-analysis of Osteopontin as a clinical cancer marker, Oncology Reports, 25, 433

    Article  PubMed  Google Scholar 

  • Wickramasinghe, S., Rincon, G., Islas-Trejo, A. and Medrano, J. F., 2012. Transcriptional profiling of bovine milk using RNA sequencing, BMC Genomics, 13, 1

    Article  Google Scholar 

  • Yudin, N. S. and Voevoda, M. I., 2015. Molecular Genetic Markers of Economically Important Traits in Dairy Cattle, Genetika, 51, 600–612

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We like to express our gratitude to Dr. Shafqat Fatima Rehmani and staff (Quality Operation Lab, UVAS, Lahore) for the technical assistance.

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan

    Sidra Manzoor, Asif Nadeem, Javed Maryam, Abu Saeed Hashmi & Muhammad Imran

  2. Virtual University of Pakistan, Lahore, 54000, Pakistan

    Masroor Ellahi Babar

Authors
  1. Sidra Manzoor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Asif Nadeem
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Javed Maryam
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Abu Saeed Hashmi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Muhammad Imran
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Masroor Ellahi Babar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Asif Nadeem.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Manzoor, S., Nadeem, A., Maryam, J. et al. Osteopontin gene polymorphism association with milk traits and its expression analysis in milk of riverine buffalo. Trop Anim Health Prod 50, 275–281 (2018). https://doi.org/10.1007/s11250-017-1426-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-017-1426-1

Keywords

Search

Navigation