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Detection of Brucella sp. infection through serological, microbiological, and molecular methods applied to buffaloes in Maranhão State, Brazil

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Abstract

The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.

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Acknowledgements

This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES - PROCAD-NF 680/2010), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa e Desenvolvimento Científico do Maranhão (FAPEMA).

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Authors and Affiliations

  1. Doutorado em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Estadual do Maranhão, São Luís, MA, Brazil

    Larissa Sarmento dos Santos, Diego Luiz dos Santos Ribeiro & Alcina Vieira de Carvalho Neta

  2. Departamento do Curso de Medicina, Universidade Ceuma, São Luís, MA, Brazil

    Joicy Cortez Sá

  3. Universidade Estadual do Maranhão, São Luís, MA, Brazil

    Nancyleni Pinto Chaves & Alcina Vieira de Carvalho Neta

  4. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Juliana Pinto da Silva Mol & Renato Lima Santos

  5. Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Tatiane Alves da Paixão

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  1. Larissa Sarmento dos Santos
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  2. Joicy Cortez Sá
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  3. Diego Luiz dos Santos Ribeiro
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  4. Nancyleni Pinto Chaves
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  5. Juliana Pinto da Silva Mol
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  7. Tatiane Alves da Paixão
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  8. Alcina Vieira de Carvalho Neta
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Correspondence to Alcina Vieira de Carvalho Neta.

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dos Santos, L.S., Sá, J.C., dos Santos Ribeiro, D.L. et al. Detection of Brucella sp. infection through serological, microbiological, and molecular methods applied to buffaloes in Maranhão State, Brazil. Trop Anim Health Prod 49, 675–679 (2017). https://doi.org/10.1007/s11250-017-1238-3

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