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Efficacy of vaccination with La Sota strain vaccine to control Newcastle disease in village chickens in Nepal

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An Erratum to this article was published on 11 March 2017

Abstract

The efficacy of vaccination with Newcastle disease (ND) La Sota and R2B (Mukteswar) modified live strain vaccines was determined by experimental challenge and with ND La Sota vaccine under field conditions in Nepal. Booster vaccination with ND La Sota vaccine after a primary vaccination with ND La Sota vaccine, induced a geometric mean titre (GMT) of 5.0 log2 haemagglutination inhibition (HI) units, compared to a GMT of 6.0 log2 HI units following booster vaccination with R2B vaccine 1 month after primary vaccination with ND La Sota vaccine. Both vaccines provided 100% protection against challenge with a local field ND strain. Furthermore, booster vaccination with ND La Sota vaccine induced protective levels of antibody after field use in villages in Jhapa, and no outbreaks of ND occurred during the study period. The ND La Sota modified live vaccine is immunogenic and efficacious and is a suitable vaccine for use in vaccination programmes in village chickens in the rural areas of Nepal.

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Abbreviations

EID50 :

Median egg infectious doses

ELISA:

Enzyme-linked immunosorbent assay

GMT:

Geometric mean titre

HI:

Haemagglutination inhibition

ICPI:

Intracerebral pathogenicity index

IBDV:

Infectious bursal disease virus

IB:

Infectious bronchitis

NDV:

Newcastle disease virus

ND:

Newcastle disease

SPF:

Specific pathogen free

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Acknowledgments

We are grateful to the excellent assistance of Sharmila Dutta, Dr S.P Gautam, Ms K. Maharjan, Dr Sita Acharya, village animal health worker Krishna, Arjun, Radhika and the staff of CBPL for their help. We would like to thank Central Veterinary Laboratory, Tripureswor for providing the field virus. This is based on research funded in part by the Bill & Melinda Gates Foundation (Grant no. OPP1009497). The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation

Author information

Authors and Affiliations

  1. Central Biological Production Laboratory, Tripureshwor, Kathmandu, Nepal

    Sulochana Shrestha

  2. GALVmed, Unit 118 & 120 B, Splendor Forum, Plot No 3, Jasola District Centre, Jasola, New Delhi, 110025, India

    Mamta Dhawan

  3. Faculty of Veterinary and Agricultural Sciences, Veterinary Clinical Centre, The University of Melbourne, 250 Princes Highway, Werribee, Victoria, 3030, Australia

    Meritxell Donadeu

  4. MCI Sante Animale, Lot 157, ZI Sud-Ouest B.P. 278, 28810, Mohammedia, Morocco

    Baptiste Dungu

Authors
  1. Sulochana Shrestha
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  2. Mamta Dhawan
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  3. Meritxell Donadeu
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  4. Baptiste Dungu
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Corresponding author

Correspondence to Sulochana Shrestha.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

All applicable international, national and/or institutional guidelines for the care and use of animals were followed. Blood sampling was carried out by veterinary surgeons and trained animal health workers. Participation in the study was on a voluntary basis, and the poultry keepers were informed about the purpose and methods of the study.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s11250-017-1260-5.

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Shrestha, S., Dhawan, M., Donadeu, M. et al. Efficacy of vaccination with La Sota strain vaccine to control Newcastle disease in village chickens in Nepal. Trop Anim Health Prod 49, 439–444 (2017). https://doi.org/10.1007/s11250-016-1205-4

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