Transgenic Research

, Volume 19, Issue 5, pp 799–808

Transgenic rabbit production with simian immunodeficiency virus-derived lentiviral vector

  • L. Hiripi
  • D. Negre
  • F.-L. Cosset
  • K. Kvell
  • T. Czömpöly
  • M. Baranyi
  • E. Gócza
  • O. Hoffmann
  • B. Bender
  • Zs. Bősze
Original Paper

DOI: 10.1007/s11248-009-9356-y

Cite this article as:
Hiripi, L., Negre, D., Cosset, FL. et al. Transgenic Res (2010) 19: 799. doi:10.1007/s11248-009-9356-y

Abstract

Transgenic rabbit is the preferred disease model of atherosclerosis, lipoprotein metabolism and cardiovascular diseases since upon introducing genetic mutations of human genes, rabbit models reflect human physiological and pathological states more accurately than mouse models. Beyond that, transgenic rabbits are also used as bioreactors to produce pharmaceutical proteins in their milk. Since in the laboratory rabbit the conventional transgenesis has worked with the same low efficiency in the last twenty five years and truly pluripotent embryonic stem cells are not available to perform targeted mutagenesis, our aim was to adapt lentiviral transgenesis to this species. A simian immunodeficiency virus based replication defective lentiviral vector was used to create transgenic rabbit through perivitelline space injection of fertilized oocytes. The enhanced green fluorescent protein (GFP) gene was placed under the ubiquitous CAG promoter. Transgenic founder rabbits showed mosaic pattern of GFP expression. Transgene integration and expression was revealed in tissues derived from all three primary germ layers. Transgene expression was detected in the developing sperm cells and could get through the germ line without epigenetic silencing, albeit with very low frequency. Our data show for the first time, that lentiviral transgenesis could be a feasible and viable alternative method to create genetically modified laboratory rabbit.

Keywords

Laboratory rabbitLentiviral transgenesisSimian immunodeficiency virusMosaic expressionGerm-line transmission

Supplementary material

11248_2009_9356_MOESM1_ESM.tif (1.6 mb)
Supplementary Fig. 1. Integrated transgene copy number determination with Southern analysis. (A) Southern blot of BamHI-digested genomic DNA isolated from SIV-CAG-eGFP transgenic founder rabbit ear samples. Lines: 1–2: founder rabbit; line 3: non-transgenic rabbit, negative control; line 4: GFP-transgenic mouse (Kvell et al. 2009), positive control. (B) The lentiviral vector carrying the CAG-GFP transgene, LTR, long terminal repeat; CPPT, polypurine tract; WPRE, woodchuck hepatitis responsive element; dotted lines, rabbit genome. (TIFF 1652 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • L. Hiripi
    • 1
  • D. Negre
    • 2
  • F.-L. Cosset
    • 2
  • K. Kvell
    • 3
  • T. Czömpöly
    • 3
  • M. Baranyi
    • 1
  • E. Gócza
    • 1
  • O. Hoffmann
    • 1
  • B. Bender
    • 1
  • Zs. Bősze
    • 1
  1. 1.Genetic Modification Program Group, Agricultural Biotechnology CenterGödöllőHungary
  2. 2.Inserm, U758, Human Virology Department Université de Lyon, Ecole Normale Supérieure de LyonLyonFrance
  3. 3.Department of Immunology and BiotechnologyUniversity of PécsPecsHungary