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Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

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Abstract

Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protein in maize kernels. The presence of the transgene was verified by Southern and western blots. Feeding chickens with kernels containing the F protein induced the production of antibodies, which conferred protection against a viral challenge. This protection was comparable to that conferred by a commercial vaccine. Possible uses of this plant-based F protein as a potential mucosal vaccine are discussed.

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Authors and Affiliations

  1. Departamento de Ingeniería Genética de Plantas, Cinvestav Campus Guanajuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, Apdo, Postal 629, Irapuato, Guanajuato, México, 36500

    Octavio Guerrero-Andrade & Miguel Angel Gómez-Lim

  2. Centro Nacional de Investigaciones Disciplinarias en Microbiología Animal, INIFAP. Carretera Federal, México-Toluca Km. 15.5, Colonia Palo Alto, México DF, CP. 05110

    Elizabeth Loza-Rubio

  3. Facultad de Química, Conjunto E, Laboratorio 116, Paseo de la Investigación Científica, Universidad Nacional Autónoma de México, México DF, CP. 04510

    Teresa Olivera-Flores

  4. Departamento de Producción Animal: Aves. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México DF, CP. 04510

    Tamás Fehérvári-Bone

Authors
  1. Octavio Guerrero-Andrade
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  2. Elizabeth Loza-Rubio
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  3. Teresa Olivera-Flores
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  4. Tamás Fehérvári-Bone
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  5. Miguel Angel Gómez-Lim
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Correspondence to Miguel Angel Gómez-Lim.

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Guerrero-Andrade, O., Loza-Rubio, E., Olivera-Flores, T. et al. Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies. Transgenic Res 15, 455–463 (2006). https://doi.org/10.1007/s11248-006-0017-0

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  • DOI: https://doi.org/10.1007/s11248-006-0017-0

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