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Modeling some mineral nutrient requirements for micropropagated wild apricot shoot cultures

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Abstract

A response surface methodology (RSM) experimental design was applied for improving micropropagation of a wild apricot, Prunus armeniaca Lam., from the mountains of Kazakhstan. In an initial study, woody plant medium (WPM) mineral nutrients [calcium nitrate, ammonium nitrate, mesos (calcium chloride, potassium phosphate and magnesium sulfate) potassium sulfate and minor nutrients] were tested in a response surface methodology (RSM) experiment. Shoot quality was the best when nitrogen and mesos (CaCl2, MgSO4, K2SO4, KH2PO4) compounds were altered. In this study an expanded mesos optimization experiment was run. Data taken included a subjective quality rating, shoot length, shoot number, leaf color and size, callus and physiological disorders. Data were analyzed by Classification and Regression Tree Analysis (CART), a data mining technique that provides specific cutoff values for data and easy to interpret data trees. The CART analysis indicated that the best quality would be with ≤2.4× WPM levels of KH2PO4 and ≤0.75× MgSO4. Shoot length was affected by K2SO4, but most shoots were of good size at any concentration. Shoot multiplication was affected by KH2PO4, but there were >5 shoots at any concentration. Leaf color was best with ≤2.41× KH2PO4 and ≤1.22× K2SO4. Based on the CART analysis, a recommendation for improved mesos compounds was developed. Each of the individual trees was analyzed and the cutoff points determined so that all the growth characteristics could be considered in the final concentrations chosen. Using the combined results from the CART analysis, the suggested medium would include WPM with CaCl2 2.7×, MgSO4 2.7×, K2SO4 0.8×, KH2PO4 0.75×.

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Acknowledgements

This study was funded by ISTC project K-1920 and USDA-ARS CRIS project 5358-21000-038-00D.

Author contributions

IK assisted with planning the study, supervised the work and assisted in writing the manuscript. ZM, TT and GM set up and ran the experiments and collected data. MA assisted with data analysis and writing the manuscript. EE assisted with data analysis. BMR assisted with planning the study, set up the experimental design, assisted with data analysis and writing the manuscript.

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Author notes

  1. Melekşen Akin

    Present address: Department of Horticulture, Agricultural Faculty, Igdir University, Igdir, Turkey

Authors and Affiliations

  1. Institute of Plant Biology and Biotechnology, Timiryazev Str. 45, 050040, Almaty, Republic of Kazakhstan

    Irina Y. Kovalchuk, Zinat Mukhitdinova, Timur Turdiyev & Gulnara Madiyeva

  2. Department of Horticulture, Oregon State University, ALS 4017, Corvallis, OR, 97331, USA

    Melekşen Akin & Barbara M. Reed

  3. Biometry Genetics Unit, Department of Animal Science, Agricultural Faculty, Igdir University, Igdir, Turkey

    Ecevit Eyduran

  4. United States Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, 33447 Peoria Road, Corvallis, OR, 97333, USA

    Barbara M. Reed

Authors
  1. Irina Y. Kovalchuk
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  2. Zinat Mukhitdinova
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  3. Timur Turdiyev
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  4. Gulnara Madiyeva
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  5. Melekşen Akin
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  6. Ecevit Eyduran
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  7. Barbara M. Reed
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Corresponding author

Correspondence to Barbara M. Reed.

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Kovalchuk, I.Y., Mukhitdinova, Z., Turdiyev, T. et al. Modeling some mineral nutrient requirements for micropropagated wild apricot shoot cultures. Plant Cell Tiss Organ Cult 129, 325–335 (2017). https://doi.org/10.1007/s11240-017-1180-0

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  • DOI: https://doi.org/10.1007/s11240-017-1180-0

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