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Characterization and expression analysis of cDNAs encoding abscisic acid 8′-hydroxylase during mulberry fruit maturation and under stress conditions

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Abstract

Abscisic acid (ABA) 8′-hydroxylase is the key enzyme in the oxidative catabolism of ABA. CYP707A genes encode (+)-ABA 8′-hydroxylases that are involved in fruit ripening and abiotic stress responses. However, there are limited reports on the functions of CYP707A genes in mulberry. This study analyzed the transcriptional expression of CYP707A genes during fruit development and their response to exogenous ABA and uniconazole (UNI) treatment. Additionally, the expression patterns of CYP707A under stress conditions were explored in mulberry. We obtained six CYP707A gene (MnCYP707A1-MnCYP707A6) sequences from Morus notabilis genome database. Multiple sequence alignment results showed high conservation within the MnCYP707A genes. The expression profiles of these genes during fruit development and under stress conditions were analyzed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). During fruit development, the expression levels of MaCYP707A1, MaCYP707A3, MaCYP707A5, and MaCYP707A6 were higher at the primary stage and then sharply declined to a lower level at the late stage. The expression of MaCYP707A1 in the fruit quickly declined at 1 d after 50 mmol/L UNI treatment. In addition, under Sodium chloride (NaCl) (0.3 % (w/v) at germination stage and 0.6 % (w/v) at seedling stage) or polyethylene glyco (PEG) 6000 (5 % (w/v) at germination stage and 20 % (w/v) at seedling stage) stress, most of the MaCYP707A genes were up-regulated, but displayed different patterns. Tissue-specific patterns of the CYP707A family appear to be universal under stress conditions. In a short, MaCYP707A genes play overlapping roles in ABA catabolism and MaCYP707A1 may be the key gene expressed during fruit maturation. Most of these genes were induced by abiotic stress, and various expression patterns were observed among the MaCYP707A in multiple mulberry tissues at different growth periods.

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Abbreviations

ABA:

Abscisic acid

CYP:

Cytochrome P450

qRT-PCR:

Quantitative reverse transcriptase polymerase chain reaction

Jialing No. 40:

Morus. atropurpurea cv. Jialing No. 40

Guiyou No. 62:

Morus. atropurpurea cv. Guiyou No. 62

UNI:

Uniconazole

NaCl:

Sodium chloride

PEG:

Polyethylene glyco

DAFB:

Days after full-bloom

ORF:

Open reading frame

cDNA:

Complementary deoxyribonucleic acid

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Acknowledgments

This research was financially supported by Fundamental Research Funds for the Central Universities (XDJK2016D024), Natural Science Foundation of China (No. 31360190), Graduate Research and Innovation Projects of Chongqing (No. CYS2015070), China Agriculture Research System(No.CARS-22).

Author contribution statement

YC (the first author) designed and performed the experiments, analyzed the results, and wrote the manuscript. PZ provided assistance with most of the experiments and data analysis. CL (the third author), AZ and JY offered scientific advises and analyzed the results. CW, ZL and PH provided assistance to prepare mulberry seedling. All of experiments were instructed by MY. All authors read and approved the manuscript.

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    Authors and Affiliations

    1. College of Biotechnology, Southwest University, Chongqing, 400715, People’s Republic of China

      Yuxiang Cai, Panpan Zhu, Changying Liu, Aichun Zhao, Jian Yu, Chuanhong Wang & Maode Yu

    2. The Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan, 661100, People’s Republic of China

      Zhengang Li & Ping Huang

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    1. Yuxiang Cai
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    Correspondence to Maode Yu.

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    Y. Cai and P. Zhu contributed equally to this work.

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    Cai, Y., Zhu, P., Liu, C. et al. Characterization and expression analysis of cDNAs encoding abscisic acid 8′-hydroxylase during mulberry fruit maturation and under stress conditions. Plant Cell Tiss Organ Cult 127, 237–249 (2016). https://doi.org/10.1007/s11240-016-1047-9

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